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Literature summary extracted from

  • Chamond, N.; Cosson, A.; Coatnoan, N.; Minoprio, P.
    Proline racemases are conserved mitogens: characterization of a Trypanosoma vivax proline racemase (2009), Mol. Biochem. Parasitol., 165, 170-179.
    View publication on PubMed

Application

EC Number Application Comment Organism
5.1.1.4 drug development enzyme has potential to be used as a drug target for this parasite-based trypanosomiasis Trypanosoma vivax

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.4 expression in Escherichia coli BL21 (DE3) with a C-terminal 6-His tag Trypanosoma vivax

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.1.4 pyrrole-2-carboxylic acid competitive inhibitor Trypanosoma vivax

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.4 106
-
 D-proline 40 mM substrate in 0.2 M sodium acetate, for 30 min at 37°C Trypanosoma vivax
5.1.1.4 145
-
 L-proline 40 mM substrate in 0.2 M sodium acetate, for 30 min at 37°C Trypanosoma vivax

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.1.4 40000
-
 recombinant enzyme, determined by SDS-PAGE Trypanosoma vivax

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.1.4 L-proline Trypanosoma vivax
-
 D-proline
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.4 Trypanosoma vivax B8LFE4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.4 by affinity chromatography using nickel-nitrilotriacetic acid-agarose columns Trypanosoma vivax

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.1.1.4 bloodstream form
-
 Trypanosoma vivax
-
5.1.1.4 epimastigote
-
 Trypanosoma vivax
-
5.1.1.4 trypomastigote
-
 Trypanosoma vivax
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.4 L-proline
-
 Trypanosoma vivax D-proline
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.1.1.4 proline racemase
-
 Trypanosoma vivax
5.1.1.4 TvHYP1
-
 Trypanosoma vivax
5.1.1.4 TvPRAC
-
 Trypanosoma vivax

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.1.4 37
-
 assay at Trypanosoma vivax

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.1.4 6
-
-
 Trypanosoma vivax

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.1.1.4 0.02
-
 pyrrole-2-carboxylic acid 0.005 mM pyrrole-2-carboxylic acid, serial concentrations of 20-160 mM L-proline Trypanosoma vivax

General Information

EC Number General Information Comment Organism
5.1.1.4 physiological function enzyme is a T-cell-independent B-cell mitogen, enzyme displays mitogenic activity towards splenic cells from euthymic Swiss mice, since addition of 0.1 mg/ml of recombinant protein promotes a 13fold increase of thymidine incorporation when compared with untreated cells, mitogenic activity of recombinant enzyme seems to be dependent on the active enzyme, since inhibition with 10 mM pyrrole-2-carboxylic acid prior to its incubation with splenocytes specifically decreases proliferation by 44%, enzyme triggers high levels of B-cell activation, terminal differentiation and antibody secretion Trypanosoma vivax