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Literature summary extracted from
- Regulation of the dauBAR Operon and Characterization of D-Amino Acid Dehydrogenase DauA in Arginine and Lysine Catabolism of Pseudomonas aeruginosa PAO1 (2009), Microbiology, 156, 60-71.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.4.99.6 | expressed as a His-tagged fusion protein | Pseudomonas aeruginosa |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.99.6 | D-arginine | substrate inhibition | Pseudomonas aeruginosa |  |
| 1.4.99.6 | D-Lysine | substrate inhibition | Pseudomonas aeruginosa | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.99.6 | 0.08 | - |
D-arginine | - |
Pseudomonas aeruginosa | |
| 1.4.99.6 | 0.19 | - |
D-Lysine | - |
Pseudomonas aeruginosa | |
| 1.4.99.6 | 0.4 | - |
D-tyrosine | - |
Pseudomonas aeruginosa | |
| 1.4.99.6 | 1.13 | - |
D-phenylalanine | - |
Pseudomonas aeruginosa | |
| 1.4.99.6 | 1.43 | - |
D-methionine | - |
Pseudomonas aeruginosa | |
| 1.4.99.6 | 1.48 | - |
D-ornithine | - |
Pseudomonas aeruginosa | |
| 1.4.99.6 | 3.52 | - |
D-histidine | - |
Pseudomonas aeruginosa | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.4.99.6 | Pseudomonas aeruginosa | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.4.99.6 | D-arginine + H2O + iodonitrotetrazolium chloride | D-arginine and D-lysine are the most effective substrates | Pseudomonas aeruginosa | 5-guanidino-2-oxopentanoate + NH3 + reduced iodonitrotetrazolium chloride | - |
? | |
| 1.4.99.6 | D-histidine + H2O + iodonitrotetrazolium chloride | - |
Pseudomonas aeruginosa | 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced iodonitrotetrazolium chloride | - |
? | |
| 1.4.99.6 | D-lysine + H2O + iodonitrotetrazolium chloride | D-arginine and D-lysine are the most effective substrates | Pseudomonas aeruginosa | 7-amino-2-oxoheptanoic acid + NH3 + reduced iodonitrotetrazolium chloride | - |
? | |
| 1.4.99.6 | D-methionine + H2O + iodonitrotetrazolium chloride | - |
Pseudomonas aeruginosa | 4-methylsulfanyl-2-oxobutanoate + NH3 + reduced iodonitrotetrazolium chloride | - |
? | |
| 1.4.99.6 | D-ornithine + H2O + iodonitrotetrazolium chloride | - |
Pseudomonas aeruginosa | ? + NH3 + reduced iodonitrotetrazolium chloride | - |
? | |
| 1.4.99.6 | D-phenylalanine + H2O + iodonitrotetrazolium chloride | - |
Pseudomonas aeruginosa | phenylpyruvate + NH3 + reduced iodonitrotetrazolium chloride | - |
? | |
| 1.4.99.6 | D-tyrosine + H2O + iodonitrotetrazolium chloride | - |
Pseudomonas aeruginosa | 3-(4-hydroxyphenyl)-2-oxopropanoate + NH3 + reduced iodonitrotetrazolium chloride | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.4.99.6 | D-amino acid dehydrogenase | - |
Pseudomonas aeruginosa |
| 1.4.99.6 | DauA | encoded by the dauBAR operon | Pseudomonas aeruginosa |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.4.99.6 | 37 | - |
assay at | Pseudomonas aeruginosa |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.99.6 | 3.6 | - |
D-tyrosine | - |
Pseudomonas aeruginosa | |
| 1.4.99.6 | 3.6 | - |
D-histidine | - |
Pseudomonas aeruginosa | |
| 1.4.99.6 | 5.3 | - |
D-phenylalanine | - |
Pseudomonas aeruginosa | |
| 1.4.99.6 | 6.2 | - |
D-ornithine | - |
Pseudomonas aeruginosa | |
| 1.4.99.6 | 6.8 | - |
D-methionine | - |
Pseudomonas aeruginosa | |
| 1.4.99.6 | 9.2 | - |
D-Lysine | - |
Pseudomonas aeruginosa | |
| 1.4.99.6 | 11.1 | - |
D-arginine | - |
Pseudomonas aeruginosa | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.4.99.6 | 8.7 | - |
assay at | Pseudomonas aeruginosa |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.99.6 | FAD | - |
Pseudomonas aeruginosa | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.99.6 | 24.1 | - |
D-arginine | - |
Pseudomonas aeruginosa | |
| 1.4.99.6 | 150 | - |
D-Lysine | - |
Pseudomonas aeruginosa | |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 1.4.99.6 | Pseudomonas aeruginosa | expression of dauBAR is specifically induced by exogenous D-arginine and D-lysine | up |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.99.6 | 1 | - |
D-histidine | - |
Pseudomonas aeruginosa | |
| 1.4.99.6 | 4.2 | - |
D-ornithine | - |
Pseudomonas aeruginosa | |
| 1.4.99.6 | 4.7 | - |
D-phenylalanine | - |
Pseudomonas aeruginosa | |
| 1.4.99.6 | 4.8 | - |
D-methionine | - |
Pseudomonas aeruginosa | |
| 1.4.99.6 | 9.1 | - |
D-tyrosine | - |
Pseudomonas aeruginosa | |
| 1.4.99.6 | 48 | - |
D-Lysine | - |
Pseudomonas aeruginosa | |
| 1.4.99.6 | 150 | - |
D-arginine | - |
Pseudomonas aeruginosa | |
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