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Literature summary extracted from
- Genetic and biochemical identification of the chorismate mutase from Corynebacterium glutamicum (2009), Microbiology, 155, 3382-3391.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.5 | additional information | the CM0819 activity is not affected by L-Phe, L-Tyr or L-Trp (0.01-10 mM), EDTA at concentrations of 0.1-2 mM does not affect CM0819 activity | Corynebacterium glutamicum |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.5 | expressed in Escherichia coli BL21(DE3) cells | Corynebacterium glutamicum |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.5 | Co2+ | 2 mM Co2+ decreases CM0819 activity by 52.4% | Corynebacterium glutamicum |  |
| 5.4.99.5 | Mn2+ | 2 mM Mn2+ decreases CM0819 activity by 38.9% | Corynebacterium glutamicum | |
| 5.4.99.5 | Ni2+ | 2 mM Ni2+ decreases CM0819 activity by 41.8% | Corynebacterium glutamicum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.5 | 2.83 | - |
chorismate | at 37°C | Corynebacterium glutamicum | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 5.4.99.5 | cytoplasm | - |
Corynebacterium glutamicum | 5737 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.5 | additional information | Mg2+ at concentrations of 0.1-2 mM does not affect CM0819 activity | Corynebacterium glutamicum |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.5 | 11300 | - |
subunit, calculated from amino acid sequence | Corynebacterium glutamicum |
| 5.4.99.5 | 12000 | - |
2 * 12000, SDS-Page | Corynebacterium glutamicum |
| 5.4.99.5 | 23500 | - |
gel filtration | Corynebacterium glutamicum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.5 | Corynebacterium glutamicum | - |
strain ATCC 13032 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.5 | His-Bind protein purification column chromatography | Corynebacterium glutamicum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.5 | Chorismate | - |
Corynebacterium glutamicum | Prephenate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.99.5 | homodimer | 2 * 12000, SDS-Page | Corynebacterium glutamicum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.5 | CM0819 | - |
Corynebacterium glutamicum |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.5 | 1.05 | - |
chorismate | at 37°C | Corynebacterium glutamicum | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 5.4.99.5 | metabolism | chorismate mutase is the first and the key enzyme that diverges the shikimate pathway to either tryptophan or phenylalanine and tyrosine | Corynebacterium glutamicum |
| 5.4.99.5 | physiological function | CM0819 significantly stimulates 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (DS2098) activity, CM0819 interacts with 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase DS2098 from Corynebacterium glutamicum and this interaction results in allosteric regulation of DS2098 synthase activity | Corynebacterium glutamicum |
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