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Literature summary extracted from

  • Ye, W.; Huo, G.; Chen, J.; Liu, F.; Yin, J.; Yang, L.; Ma, X.
    Heterologous expression of the Bacillus subtilis (natto) alanine dehydrogenase in Escherichia coli and Lactococcus lactis (2009), Microbiol. Res., 165, 268-275.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.4.1.1 nutrition Lactococcus lactis strain NZ9000 expressing the enzyme from Bacillus subtilis can be used in imporvement of dairy fermentation for developing healthy yogurts with sweet taste or other fermented dairy foods Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.1 gene ald, functional heterologous expression in Escherichia coli strain JM109, as GST-tagged protein, and in Lactococcus lactis strain NZ9000 Bacillus subtilis
1.4.99.6 recombinantly expressed in Escherichia coli Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.99.6 benzoic acid 0.1 mM, 98% inhibition Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
1.4.99.6 iodoacetamide 0.1 mM, 65.8% inhibition Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
1.4.99.6 iodoacetate 0.1 mM, 61.8% inhibition Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
1.4.99.6 p-hydroxymercuribenzoate 0.1 mM, 78.6% inhibition Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.99.6 0.0082
-
D-proline Vmax: 12.3 micromol/min/mg Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
1.4.99.6 40.2
-
D-proline Vmax: 25 micromol/min/mg Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.1 70000
-
x * 70000, recombinant GST-tagged enzyme, SDS-PAGE Bacillus subtilis
1.4.99.6 44000
-
SDS-PAGE Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.1 pyruvate + NH3 + NADH + H+ Bacillus subtilis
-
L-alanine + H2O + NAD+
-
?
1.4.1.1 pyruvate + NH3 + NADH + H+ Bacillus subtilis natto
-
L-alanine + H2O + NAD+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.1 Bacillus subtilis
-
gene ald
-
1.4.1.1 Bacillus subtilis natto
-
gene ald
-
1.4.1.1 no activity in Lactococcus lactis
-
strain NZ9000
-
1.4.99.6 Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504 A3KEZ1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.1 recombinant GST-tagged enzyme from Escherichia coli strain JM109 by glutathione affinity chrmatography Bacillus subtilis
1.4.99.6 using a DEAE-Toyopearl column Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.99.6 0.42
-
using D-serine as a substrate Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
1.4.99.6 1.25
-
using D-phenylalanine as a substrate Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
1.4.99.6 2.29
-
using D-alanine as a substrate Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
1.4.99.6 6.22
-
using D-proline as a substrate Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.1 pyruvate + NH3 + NADH + H+
-
Bacillus subtilis L-alanine + H2O + NAD+
-
?
1.4.1.1 pyruvate + NH3 + NADH + H+
-
Bacillus subtilis natto L-alanine + H2O + NAD+
-
?
1.4.99.6 D-alanine + H2O + 2,6-dichlorophenolindophenol
-
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504 pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
1.4.99.6 D-phenylalanine + H2O + 2,6-dichlorophenolindophenol
-
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504 phenylpyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
1.4.99.6 D-proline + H2O + 2,6-dichlorophenolindophenol
-
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504 DELTA1-pyrroline-2-carboxylate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?
1.4.99.6 D-proline + H2O + coenzyme Q1
-
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504 DELTA1-pyrroline-2-carboxylate + NH3 + reduced coenzyme Q1
-
?
1.4.99.6 D-serine + H2O + 2,6-dichlorophenolindophenol
-
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504 3-hydroxy-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
?

Subunits

EC Number Subunits Comment Organism
1.4.1.1 ? x * 70000, recombinant GST-tagged enzyme, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
1.4.1.1 ALADH
-
Bacillus subtilis
1.4.99.6 D-amino acid dehydrogenase
-
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504
1.4.99.6 DadA
-
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.1 30
-
-
Bacillus subtilis
1.4.99.6 37
-
optimal temperature and assay at Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.4.1.1 30 42 assay at Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.99.6 8
-
optimal pH and assay at Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.1 NADH
-
Bacillus subtilis
1.4.99.6 FAD
-
Helicobacter pylori NCTC 11637 = CCUG 17874 = ATCC 43504