EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.2.2.3 | (NH4)2SO4 | (NH4)2SO4 at 0.2 M increases the original activity of isoform A9mT 23.5times | Vibrio sp. | |
4.2.2.3 | CH3COO- NH4+ | CH3COONH4 at 0.2 M increases the original activity of isoform A9mT 20.9times | Vibrio sp. | |
4.2.2.3 | CH3COONa | CH3COONa at 0.2 M increases the original activity of isoform A9mT 21times | Vibrio sp. | |
4.2.2.3 | CsCl | CsCl at 0.2 M increases the original activity of isoform A9mT 25times | Vibrio sp. | |
4.2.2.3 | KCl | KCl at 0.2 M increases the original activity of isoform A9mT 24.9times | Vibrio sp. | |
4.2.2.3 | LiCl | LiCl at 0.2 M increases the original activity of isoform A9mT 18.8times | Vibrio sp. | |
4.2.2.3 | additional information | 5 mM EDTA and EGTA do not influence enzyme activity of isoform A9mT | Vibrio sp. | |
4.2.2.3 | NaBr | NaBr at 0.2 M increases the original activity of isoform A9mT 21.8times | Vibrio sp. | |
4.2.2.3 | NaCl | recombinant isoform A9mT is remarkably activated in the presence of NaCl, when 0.4 M NaCl is added to the reaction mixture, the activity is increased maximally to 24times the original activity, the activity gradually decreases at higher NaCl concentrations, but is still activated 20times at 0.8 M and 8 times at 1.6 M NaCl | Vibrio sp. | |
4.2.2.3 | NaF | NaF at 0.2 M increases the original activity of isoform A9mT 21.7times | Vibrio sp. | |
4.2.2.3 | NH4Cl | NH4Cl at 0.2 M increases the original activity of isoform A9mT 22.7times | Vibrio sp. | |
4.2.2.3 | Nonidet P40 | the enzyme is moderately activated by Nonidet P40 by 134% | Vibrio sp. | |
4.2.2.3 | RbCl | RbCl at 0.2 M increases the original activity of isoform A9mT 25times | Vibrio sp. | |
4.2.2.3 | Tween 20 | the enzyme is moderately activated by Tween 20 by 139% | Vibrio sp. |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.2.3 | expressed in Escherichia coli DH5alpha cells | Vibrio sp. |
4.2.2.3 | expressed in Escherichia coli DH5alpha cells | Agarivorans sp. |
4.2.2.3 | expression in Escherichia coli | Vibrio sp. |
4.2.2.11 | expression in Escherichia coli | Vibrio sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.2.3 | Co2+ | isoform A9mT is inhibited by 14% at 1 mM | Vibrio sp. | |
4.2.2.3 | Cu2+ | isoform A9mT is inhibited by 16% at 1 mM | Vibrio sp. | |
4.2.2.3 | NaCl | in the presence of 0.2 M NaCl, the residual activities of isoform A9mL from pH 6 to 10 are decreased 40-75% of those observed in the absence of NaCl | Vibrio sp. | |
4.2.2.3 | Ni2+ | isoform A9mT is inhibited by 35% at 1 mM | Vibrio sp. | |
4.2.2.3 | SDS | strongly inhibits the enzyme activity by 90% | Vibrio sp. | |
4.2.2.3 | Sr2+ | isoform A9mT is inhibited by 14% at 1 mM | Vibrio sp. | |
4.2.2.3 | Zn2+ | isoform A9mT is inhibited by 19% at 1 mM | Vibrio sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.2.2.11 | intracellular | - |
Vibrio sp. | 5622 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.2.3 | Cs+ | 0.2 M, 2500% of initial activity | Vibrio sp. | |
4.2.2.3 | K+ | 0.2 M, 2500% of initial activity | Vibrio sp. | |
4.2.2.3 | Li+ | 0.2 M, 1880% of initial activity | Vibrio sp. | |
4.2.2.3 | additional information | Sn2+, Ca2+, Mn2+, and Mg2+ do not influence enzyme activity of isoform A9mT | Vibrio sp. | |
4.2.2.3 | Na+ | 0.2 M, 2200% of initial activity | Vibrio sp. | |
4.2.2.3 | NH4+ | 0.2 M, 2270% of initial activity | Vibrio sp. | |
4.2.2.3 | Rb+ | 0.2 M, 2500% of initial activity | Vibrio sp. | |
4.2.2.11 | Cs+ | 0.2 M, 2500% of initial activity | Vibrio sp. | |
4.2.2.11 | K+ | 0.2 M, 2500% of initial activity | Vibrio sp. | |
4.2.2.11 | Li+ | 0.2 M, 1880% of initial activity | Vibrio sp. | |
4.2.2.11 | Na+ | 0.2 M, 2200% of initial activity | Vibrio sp. | |
4.2.2.11 | NH4+ | 0.2 M, 2270% of initial activity | Vibrio sp. | |
4.2.2.11 | Rb+ | 0.2 M, 2500% of initial activity | Vibrio sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.2.3 | 25010 | - |
isoform A9mC, calculated from amino acid sequence | Vibrio sp. |
4.2.2.3 | 28000 | - |
isoform A9mT, SDS-PAGE | Vibrio sp. |
4.2.2.3 | 29640 | - |
isoform A9mT, calculated from amino acid sequence | Vibrio sp. |
4.2.2.3 | 29643 | - |
1 * 29643, calculated, 1 * 28000, SDS-PAGE | Vibrio sp. |
4.2.2.3 | 30218 | - |
x * 30218, calculated | Agarivorans sp. |
4.2.2.3 | 30220 | - |
isoform A1mU, calculated from amino acid sequence | Agarivorans sp. |
4.2.2.3 | 31000 | - |
gel filtration | Vibrio sp. |
4.2.2.3 | 31000 | - |
isoform A9mT, Bio-Gel-A0.5 m gel filtration | Vibrio sp. |
4.2.2.3 | 54430 | - |
isoform A9mL, calculated from amino acid sequence | Vibrio sp. |
4.2.2.11 | 25009 | - |
x * 25009, calculated | Vibrio sp. |
4.2.2.11 | 28000 | - |
1 * 29643, calculated, 1 * 28000, SDS-PAGE | Vibrio sp. |
4.2.2.11 | 29643 | - |
1 * 29643, calculated, 1 * 28000, SDS-PAGE | Vibrio sp. |
4.2.2.11 | 31000 | - |
gel filtration | Vibrio sp. |
4.2.2.11 | 54426 | - |
x * 54426, calculated | Vibrio sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.2.3 | Agarivorans sp. | C4TIN8 | - |
- |
4.2.2.3 | Agarivorans sp. JAM-A1m | C4TIN8 | - |
- |
4.2.2.3 | Vibrio sp. | - |
sequence contains a putative signal sequence of 23 amino acids | - |
4.2.2.3 | Vibrio sp. | C4TJD2 | - |
- |
4.2.2.3 | Vibrio sp. | C4TJD2 | isoform A9mT; strain JAM-A9m | - |
4.2.2.3 | Vibrio sp. | C4TJD3 | isoform A9mC; strain JAM-A9m | - |
4.2.2.3 | Vibrio sp. | C4TJD4 | isoform A9mL; strain JAM-A9m | - |
4.2.2.3 | Vibrio sp. A9m | - |
sequence contains a putative signal sequence of 23 amino acids | - |
4.2.2.3 | Vibrio sp. A9m | C4TJD2 | - |
- |
4.2.2.3 | Vibrio sp. A9m | C4TJD2 | isoform A9mT; strain JAM-A9m | - |
4.2.2.3 | Vibrio sp. A9m | C4TJD3 | isoform A9mC; strain JAM-A9m | - |
4.2.2.3 | Vibrio sp. A9m | C4TJD4 | isoform A9mL; strain JAM-A9m | - |
4.2.2.11 | Vibrio sp. | C4TJD2 | sequence contains a putative signal sequence of 23 amino acids | - |
4.2.2.11 | Vibrio sp. | C4TJD3 | - |
- |
4.2.2.11 | Vibrio sp. | C4TJD4 | sequence contains a putative 26-amino-acid signal peptide | - |
4.2.2.11 | Vibrio sp. A9m | C4TJD2 | sequence contains a putative signal sequence of 23 amino acids | - |
4.2.2.11 | Vibrio sp. A9m | C4TJD3 | - |
- |
4.2.2.11 | Vibrio sp. A9m | C4TJD4 | sequence contains a putative 26-amino-acid signal peptide | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
4.2.2.3 | proteolytic modification | sequence contains a putative signal sequence of 23 amino acids | Vibrio sp. |
4.2.2.11 | proteolytic modification | sequence contains a putative 26-amino-acid signal peptide | Vibrio sp. |
4.2.2.11 | proteolytic modification | sequence contains a putative signal sequence of 23 amino acids | Vibrio sp. |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.2.3 | DEAE-Toyopearl column chromatography | Vibrio sp. |
4.2.2.3 | DEAE-Toyopearl column chromatography | Agarivorans sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.2.3 | 1401 | - |
pH 7.5, 30°C | Vibrio sp. |
4.2.2.11 | 1401 | - |
pH 7.5, 30°C | Vibrio sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.2.3 | alginate | preferably degrades M blocks | Agarivorans sp. | ? | - |
? | |
4.2.2.3 | alginate | preferably degrades M blocks | Agarivorans sp. JAM-A1m | ? | - |
? | |
4.2.2.3 | alginate | - |
Vibrio sp. | unsaturated algino-oligosaccharides | - |
? | |
4.2.2.3 | alginate | - |
Agarivorans sp. | unsaturated algino-oligosaccharides | - |
? | |
4.2.2.3 | alginate | isoform A9mT favorably degrades mannuronate polymer in alginate, the relative activities for alginate, beta-D-mannuronic acid, 1,4 linked alpha-L-guluronic acid, and 1,4 linked alpha-L-guluronic acid/beta-D-mannuronic acid blocks are 100%, 75%, 21%, and 15%, respectively, under the standard assay conditions at pH 7.5 | Vibrio sp. | unsaturated algino-oligosaccharides | - |
? | |
4.2.2.3 | alginate | isoform A9mT favorably degrades mannuronate polymer in alginate, the relative activities for alginate, beta-D-mannuronic acid, 1,4 linked alpha-L-guluronic acid, and 1,4 linked alpha-L-guluronic acid/beta-D-mannuronic acid blocks are 100%, 75%, 21%, and 15%, respectively, under the standard assay conditions at pH 7.5 | Vibrio sp. A9m | unsaturated algino-oligosaccharides | - |
? | |
4.2.2.3 | alginate | - |
Vibrio sp. A9m | unsaturated algino-oligosaccharides | - |
? | |
4.2.2.3 | alginate | - |
Agarivorans sp. JAM-A1m | unsaturated algino-oligosaccharides | - |
? | |
4.2.2.3 | beta-D-mannuronyl linkage in alginate | preferably degrades the M block over the G block in alginate | Vibrio sp. | ? | - |
? | |
4.2.2.3 | beta-D-mannuronyl linkage in alginate | preferably degrades the M block over the G block in alginate | Vibrio sp. A9m | ? | - |
? | |
4.2.2.3 | additional information | the relative activities for alginate, M, G, and GM blocks are 100%, 75%, 21%, and 15%, respectively | Vibrio sp. | ? | - |
? | |
4.2.2.3 | additional information | the relative activities for alginate, M, G, and GM blocks are 100%, 75%, 21%, and 15%, respectively | Vibrio sp. A9m | ? | - |
? | |
4.2.2.3 | poly(beta-D-mannuronate) | - |
Agarivorans sp. | ? | - |
? | |
4.2.2.3 | poly(beta-D-mannuronate) | - |
Agarivorans sp. JAM-A1m | ? | - |
? | |
4.2.2.11 | alpha-L-guluronosyl linkage in alginate | preferably degrades G blocks | Vibrio sp. | ? | - |
? | |
4.2.2.11 | alpha-L-guluronosyl linkage in alginate | preferably degrades the M block to the G block in alginate | Vibrio sp. | ? | - |
? | |
4.2.2.11 | alpha-L-guluronosyl linkage in alginate | preferably degrades the M block to the G block in alginate | Vibrio sp. A9m | ? | - |
? | |
4.2.2.11 | alpha-L-guluronosyl linkage in alginate | preferably degrades G blocks | Vibrio sp. A9m | ? | - |
? | |
4.2.2.11 | additional information | the relative activities for alginate, M, G, and GM blocks are 100%, 75%, 21%, and 15%, respectively | Vibrio sp. | ? | - |
? | |
4.2.2.11 | additional information | the relative activities for alginate, M, G, and GM blocks are 100%, 75%, 21%, and 15%, respectively | Vibrio sp. A9m | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.2.3 | ? | x * 30218, calculated | Agarivorans sp. |
4.2.2.3 | monomer | 1 * 31000, isoform A9mT, Bio-Gel-A0.5 m gel filtration | Vibrio sp. |
4.2.2.3 | monomer | 1 * 29643, calculated, 1 * 28000, SDS-PAGE | Vibrio sp. |
4.2.2.11 | ? | x * 25009, calculated | Vibrio sp. |
4.2.2.11 | ? | x * 54426, calculated | Vibrio sp. |
4.2.2.11 | monomer | 1 * 29643, calculated, 1 * 28000, SDS-PAGE | Vibrio sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.2.3 | A1mU | isoform | Agarivorans sp. |
4.2.2.3 | A9mC | isoform | Vibrio sp. |
4.2.2.3 | A9mL | isoform | Vibrio sp. |
4.2.2.3 | A9mT | - |
Vibrio sp. |
4.2.2.3 | A9mT | isoform | Vibrio sp. |
4.2.2.3 | alg | - |
Agarivorans sp. |
4.2.2.3 | alginate lyase | - |
Vibrio sp. |
4.2.2.3 | alginate lyase | - |
Agarivorans sp. |
4.2.2.11 | A9mC | - |
Vibrio sp. |
4.2.2.11 | A9mL | - |
Vibrio sp. |
4.2.2.11 | A9mT | - |
Vibrio sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.2.3 | 30 | - |
recombinant isoform A9mT, around 30°C in the presence of 0.2 M NaCl at pH 7.5 | Vibrio sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.2.3 | 5 | 50 | the relative activities of isoform A9mT at 10°C and 2°C are around 45% and 30% of the maximal activity at 30°C, respectively, isoform A9mT shows the highest thermal stability in the presence of NaCl, recombinant isoform A9mT is rapidly inactivated above 30°C when the enzyme is incubated at 5°C to 50°C for 15 min in 100 mM Tris-HCl buffer (pH 7.5) with or without 0.2 M NaCl and the enzyme is rapidly inactivated when incubated at above 40°C in the absence of NaCl | Vibrio sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.2.3 | 7.6 | 9 | - |
Vibrio sp. |
4.2.2.3 | 7.6 | 9 | recombinant isoform A9mT, in 100 mM Tris-HCl buffer, MOPS buffer, and glycine-NaOH buffer in the presence of 0.2 M NaCl | Vibrio sp. |
4.2.2.3 | 8 | 9 | crude recombinant A9mL isozyme | Vibrio sp. |
4.2.2.11 | 7.6 | 9 | - |
Vibrio sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.2.2.11 | 8 | 9 | - |
Vibrio sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
4.2.2.3 | 7 | 10 | isoform A9mT is stable at pH 7.0-10.0 in various 20 mM buffers without NaCl after incubation at 30°C for 15 min | Vibrio sp. |
4.2.2.3 | 9.5 | 10 | the relative activities at pH 9.5 and 10 in glycine-NaOH buffer are approximately 50% and 20%, respectively, of the maximal activity of isoform A9mL | Vibrio sp. |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
4.2.2.3 | Vibrio sp. | isoform A9mC, calculated from amino acid sequence | - |
4.7 |
4.2.2.3 | Vibrio sp. | isoform A9mL, calculated from amino acid sequence | - |
4.9 |
4.2.2.3 | Agarivorans sp. | calculated | - |
6.4 |
4.2.2.3 | Agarivorans sp. | isoform A1mU, calculated from amino acid sequence | - |
6.4 |
4.2.2.3 | Vibrio sp. | calculated | - |
7.2 |
4.2.2.3 | Vibrio sp. | isoform A9mT, calculated from amino acid sequence | - |
7.2 |
4.2.2.11 | Vibrio sp. | calculated | - |
4.7 |
4.2.2.11 | Vibrio sp. | calculated | - |
4.9 |
4.2.2.11 | Vibrio sp. | calculated | - |
7.2 |