BRENDA - Enzyme Database show

Structural analysis of lipocalin-type prostaglandin D synthase complexed with biliverdin by small-angle X-ray scattering and multi-dimensional NMR

Miyamoto, Y.; Nishimura, S.; Inoue, K.; Shimamoto, S.; Yoshida, T.; Fukuhara, A.; Yamada, M.; Urade, Y.; Yagi, N.; Ohkubo, T.; Inui, T.; J. Struct. Biol. 169, 209-218 (2010)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.3.99.2
for expression in Escherichia coli BL21DE3 cells
Mus musculus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
5.3.99.2
DELTA1-24_C65A L-PGDS
mutant, preserves a disulfide bond between Cys89 and Cys186
Mus musculus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.2
20000
-
L-PGDS
Mus musculus
5.3.99.2
21000
-
L-PGDS bound to biliverdin
Mus musculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.2
Prostaglandin H2
Mus musculus
-
Prostaglandin D2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.2
Mus musculus
O09114
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.99.2
the DELTA1-24_C65A L-PGDS mutant is purified on a glutathione-Sepharose 4B column and incubated with thrombin to release the L-PGDS, the protein is further purified by gel filtration
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.2
Prostaglandin H2
-
705407
Mus musculus
Prostaglandin D2
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.2
for expression in Escherichia coli BL21DE3 cells
Mus musculus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
5.3.99.2
DELTA1-24_C65A L-PGDS
mutant, preserves a disulfide bond between Cys89 and Cys186
Mus musculus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.2
20000
-
L-PGDS
Mus musculus
5.3.99.2
21000
-
L-PGDS bound to biliverdin
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.2
Prostaglandin H2
Mus musculus
-
Prostaglandin D2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.2
the DELTA1-24_C65A L-PGDS mutant is purified on a glutathione-Sepharose 4B column and incubated with thrombin to release the L-PGDS, the protein is further purified by gel filtration
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.2
Prostaglandin H2
-
705407
Mus musculus
Prostaglandin D2
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
5.3.99.2
physiological function
lipocalin-type prostaglandin D synthase acts as both a PGD2 synthase and an extracellular transporter for small lipophilic molecules
Mus musculus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
5.3.99.2
physiological function
lipocalin-type prostaglandin D synthase acts as both a PGD2 synthase and an extracellular transporter for small lipophilic molecules
Mus musculus