Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wang, L.; Xu, D.X.; Lv, Y.G.; Zhang, H.
    Purification and biochemical characterisation of a novel glutamate decarboxylase from rice bran (2010), J. Sci. Food Agric., 90, 1027-1033.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.15 Ag+ 58.3% residual activity at 2 mM Oryza sativa
4.1.1.15 Al3+ 91.2% residual activity at 2 mM Oryza sativa
4.1.1.15 CH3COOH 57.4% residual activity at 2 mM Oryza sativa
4.1.1.15 EDTA 91.2% residual activity at 2 mM Oryza sativa
4.1.1.15 Hg2+ 37.7% residual activity at 2 mM Oryza sativa
4.1.1.15 Mg2+ 90.1% residual activity at 2 mM Oryza sativa
4.1.1.15 Mn2+ 92.6% residual activity at 2 mM Oryza sativa
4.1.1.15 SDS 66.9% residual activity at 40 mM Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.15 0.0274
-
L-glutamate
-
Oryza sativa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.15 Ca2+ GAD activity is significantly enhanced in the presence of Ca2+ Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.15 48000
-
SDS-PAGE Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.15 Oryza sativa Q9AR41
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.15 ammonium sulfate fractionation, DEAE Sepharose column chromatography, and Superdex 200 gel filtration Oryza sativa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.15 7.68
-
-
Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.15 L-glutamate
-
Oryza sativa 4-aminobutanoate + CO2
-
?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.15 GAD
-
Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.15 40
-
-
Oryza sativa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.1.15 30 50 retains 78 and 69% of its maximum activity at 30 and 50°C, respectively Oryza sativa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.15 60 70 the enzyme is significantly inactivated when the temperature is increased to 60°C, with 36 and 49% of the original activity being lost after 30 and 60 min of incubation respectively, and is completely inactivated at 70°C Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.15 5.5
-
-
Oryza sativa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.15 5 9 retains 80% of its original activity in the pH range 5-9 Oryza sativa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.1.15 10
-
beyond pH range 5.0-9.0 a sharp decline in enzyme stability is observed, and negligible activity is detected at pH above 10 Oryza sativa

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.15 pyridoxal 5'-phosphate
-
Oryza sativa