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Literature summary extracted from
- Hydrogen peroxide as an effecter on the inactivation of particulate methane monooxygenase under aerobic conditions (2009), J. Mol. Catal. B, 57, 211-215.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.18.3 | catalase | increases pMMO activity, catalyzes decomposition of H2O2, on pMMO activity | Methylosinus trichosporium OB3b |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.18.3 | H2O2 | reversible inhibition of pMMO with H2O2 upon treatment of pMMO with H2O2 followed by the addition of catalase. H2O2 re-oxidizes the type 2 copper in pMMO reduced with duroquinol | Methylosinus trichosporium OB3b |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.18.3 | membrane | - |
Methylosinus trichosporium OB3b | 16020 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.18.3 | Cu2+ | purified pMMO contains 2-3 coppers | Methylosinus trichosporium OB3b | |
| 1.14.18.3 | additional information | purified pMMO contains no iron per pMMO protomer | Methylosinus trichosporium OB3b |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.18.3 | Methylosinus trichosporium OB3b | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.18.3 | by centrifugation and gel filtration | Methylosinus trichosporium OB3b |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.18.3 | methane + reduced acceptor + H* + O2 | in the presence of pMMO substrate methane, the H2O2 formation is diminished, which is likely to be caused by the consumption of electrons by methane oxidation | Methylosinus trichosporium OB3b | methanol + acceptor + H2O | - |
? | |
| 1.14.18.3 | additional information | duroquinol, an electron donor for pMMO may induce the formation of H2O2 by pMMO under aerobic conditions | Methylosinus trichosporium OB3b | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.18.3 | heterotrimer | SDS-PAGE | Methylosinus trichosporium OB3b |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.18.3 | particulate methane monooxygenase | - |
Methylosinus trichosporium OB3b |
| 1.14.18.3 | pMMO | - |
Methylosinus trichosporium OB3b |
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Release 2023.1 (January 2023)
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