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Literature summary extracted from

  • Demirci, H.; Belardinelli, R.; Seri, E.; Gregory, S.T.; Gualerzi, C.; Dahlberg, A.E.; Jogl, G.
    Structural rearrangements in the active site of the Thermus thermophilus 16S rRNA methyltransferase KsgA in a binary complex with 5'-methylthioadenosine (2009), J. Mol. Biol., 388, 271-282.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.182 full-length ksgA gene is cloned into the expression vector pET26b and overexpressed in Escherichia coli strain BL21Star Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.182 microbatch technique under oil at 4°C, crystal structures of KsgA in the apo form and with 5’-methylthioadenosine or adenosine bound in the cofactor-binding site Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.182 Mg2+ methylation of 30S ribosomal subunits by Thermus thermophilus KsgA is more efficient at low concentrations of magnesium ions suggesting that partially unfolded RNA is the preferred substrate Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.182 Thermus thermophilus Q5SM60
-
-
2.1.1.182 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 Q5SM60
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.182
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.182 4 S-adenosyl-L-methionine + adenine1518/adenine1519 in 16S rRNA KsgA introduces the most highly conserved ribosomal RNA modification, the dimethylation of A1518 and A1519 of 16S rRNA. Methylation of 30S ribosomal subunits by Thermus thermophilus KsgA is more efficient at low concentrations of magnesium ions suggesting that partially unfolded RNA is the preferred substrate Thermus thermophilus 4 S-adenosyl-L-homocysteine + N6-dimethyladenine1518/N6-dimethyladenine1519 in 16S rRNA
-
 ?
2.1.1.182 4 S-adenosyl-L-methionine + adenine1518/adenine1519 in 16S rRNA KsgA introduces the most highly conserved ribosomal RNA modification, the dimethylation of A1518 and A1519 of 16S rRNA. Methylation of 30S ribosomal subunits by Thermus thermophilus KsgA is more efficient at low concentrations of magnesium ions suggesting that partially unfolded RNA is the preferred substrate Thermus thermophilus HB8 / ATCC 27634 / DSM 579 4 S-adenosyl-L-homocysteine + N6-dimethyladenine1518/N6-dimethyladenine1519 in 16S rRNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.182 KsgA
-
 Thermus thermophilus

General Information

EC Number General Information Comment Organism
2.1.1.182 malfunction loss of this dimethylation confers resistance to the antibiotic kasugamycin Thermus thermophilus