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Literature summary extracted from

  • Priyadharshini, R.; Hemalatha, D.; Gunasekaran, P.
    Role of Val289 residue in the alpha-amylase of Bacillus amyloliquefaciens MTCC 610: An analysis by site directed mutagenesis (2010), J. Microbiol. Biotechnol., 20, 563-568.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.1 expressed in Escherichia coli XL1 Blue cells Bacillus amyloliquefaciens

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.1 V289D the mutation results in complete loss of the alpha-amylase activity Bacillus amyloliquefaciens
3.2.1.1 V289E the mutation results in complete loss of the alpha-amylase activity Bacillus amyloliquefaciens
3.2.1.1 V289F the mutant shows 48.9% activity compared to the wild type enzyme Bacillus amyloliquefaciens
3.2.1.1 V289G the mutant shows 14.5% activity compared to the wild type enzyme Bacillus amyloliquefaciens
3.2.1.1 V289I the mutant shows 20% more hydrolytic activity than the wild type enzyme Bacillus amyloliquefaciens
3.2.1.1 V289L the mutant shows 36.4% activity compared to the wild type enzyme Bacillus amyloliquefaciens
3.2.1.1 V289P the mutant shows 2.2% activity compared to the wild type enzyme Bacillus amyloliquefaciens
3.2.1.1 V289R the mutation results in complete loss of the alpha-amylase activity Bacillus amyloliquefaciens
3.2.1.1 V289S the mutant shows 5% activity compared to the wild type enzyme Bacillus amyloliquefaciens
3.2.1.1 V289Y the mutant shows 9.2% activity compared to the wild type enzyme, the mutant has acquired a transglycosylation activity, which results in the change of product profile of the reaction, giving a longer oligosaccharide Bacillus amyloliquefaciens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Bacillus amyloliquefaciens
-
-
-
3.2.1.1 Bacillus amyloliquefaciens MTCC 610
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 maltotetraose + H2O
-
 Bacillus amyloliquefaciens ?
-
 ?
3.2.1.1 maltotetraose + H2O
-
 Bacillus amyloliquefaciens MTCC 610 ?
-
 ?
3.2.1.1 starch + H2O
-
 Bacillus amyloliquefaciens malto-oligosaccharides
-
 ?
3.2.1.1 starch + H2O
-
 Bacillus amyloliquefaciens MTCC 610 malto-oligosaccharides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.1 AmyA
-
 Bacillus amyloliquefaciens
3.2.1.1 endo-1,4-alpha-D-glucan glucohydrolase
-
 Bacillus amyloliquefaciens