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Literature summary extracted from

  • Montero-Moran, G.; Caviglia, J.M.; McMahon, D.; Rothenberg, A.; Subramanian, V.; Xu, Z.; Lara-Gonzalez, S.; Storch, J.; Carman, G.M.; Brasaemle, D.L.
    CGI-58/ABHD5 is a coenzyme A-dependent lysophosphatidic acid acyltransferase (2009), J. Lipid Res., 51, 709-719.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.1.51 medicine expression of isoform CGI-58 in fibroblasts from humans with Chanarin-Dorfman increases the incorporation of fatty acids released from the lipolysis of stored triacylglycerols into phospholipids Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.51 expression in Escherichia coli Mus musculus
2.3.1.51 expression of active His6-tagged CGI-58 in Escherichia coli Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.51 additional information recombinant expression of murine CGI-58 in fibroblasts from humans with Chanarin-Dorfman syndrome increases the incorporation of radiolabeled fatty acids released from the lipolysis of stored triacylglycerols into phospholipids Mus musculus
2.3.1.51 additional information recombinant expression of murine CGI-58 in fibroblasts from humans with Chanarin-Dorfman syndrome increases the incorporation of radiolabeled fatty acids released from the lipolysis of stored triacylglycerols into phospholipids Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.51 CaCl2 1 microM, 60% inhibition. Addition of EDTA restores activity Mus musculus
2.3.1.51 MgCl2 1 microM, 35% inhibition. Addition of EDTA restores activity Mus musculus
2.3.1.51 Triton X-100 0.4 mM, inhibition Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.51 0.0048
-
 oleoyl-CoA pH 7.5, 30°C Mus musculus
2.3.1.51 0.018
-
 1-oleoyl-sn-glycerol 3-phosphate pH 7.5, 30°C Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate Mus musculus i.e. lysophosphatidic acid CoA + 1,2-diacyl-sn-glycerol 3-phosphate i.e. phosphatidic acid ?
2.3.1.51 additional information Mus musculus CGI-58 is a CoA-dependent lysophosphatidic acid acyltransferase that channels fatty acids released from the hydrolysis of stored triacylglycerols into phospholipids, overview ?
-
 ?
2.3.1.51 additional information Homo sapiens CGI-58 is a CoA-dependent lysophosphatidic acid acyltransferase that channels fatty acids released from the hydrolysis of stored triacylglycerols into phospholipids, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.51 Homo sapiens
-
-
-
2.3.1.51 Mus musculus
-
-
-
2.3.1.51 Mus musculus Q9DBL9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate i.e. lysophosphatidic acid Mus musculus CoA + 1,2-diacyl-sn-glycerol 3-phosphate i.e. phosphatidic acid ?
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate i.e. lysophosphatidic acid, the recombinant CGI-58 shows preference for arachidonoyl-CoA and oleoyl-CoA, and slight preference for 1-oleoyl lysophosphatidic acid over 1-palmitoyl, 1-stearoyl, or 1-arachidonoyl lysophosphatidic acid Mus musculus CoA + 1,2-diacyl-sn-glycerol 3-phosphate i.e. phosphatidic acid ?
2.3.1.51 arachidonoyl-CoA + 1-arachidonoyl-2-lyso-sn-phosphatidylcholine
-
 Mus musculus CoA + 1,2-diarachidonoyl-sn-phosphatidylcholine
-
 ?
2.3.1.51 arachidonoyl-CoA + 1-arachidonoyl-sn-glycerol 3-phosphate slight preference for 1-oleoyl lysophosphatidic acid over 1-palmitoyl, 1-stearoyl, or 1-arachidonoyl lysophosphatidic acid Mus musculus CoA + 1,2-diarachidonoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 arachidonoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate and oleoyl-CoA, preferred substrates Mus musculus CoA + 1-oleoyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 arachidonoyl-CoA + 1-oleoyl-sn-lysophosphatidylcholine
-
 Mus musculus CoA + 1-oleoyl-2-arachidonoylphosphatidylcholine
-
 ?
2.3.1.51 arachidonoyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate slight preference for 1-oleoyl lysophosphatidic acid over 1-palmitoyl, 1-stearoyl, or 1-arachidonoyl lysophosphatidic acid Mus musculus CoA + 1-palmitoyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 arachidonoyl-CoA + 1-palmitoyl-sn-lysophosphatidylcholine
-
 Mus musculus CoA + 1-palmitoyl-2-arachidonoylphosphatidylcholine
-
 ?
2.3.1.51 arachidonoyl-CoA + 1-stearoyl-sn-glycerol 3-phosphate slight preference for 1-oleoyl lysophosphatidic acid over 1-palmitoyl, 1-stearoyl, or 1-arachidonoyl lysophosphatidic acid Mus musculus CoA + 1-oleoyl-2-stearoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 arachidonoyl-CoA + 1-stearoyl-sn-lysophosphatidylcholine
-
 Mus musculus CoA + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphocholine
-
 ?
2.3.1.51 additional information CGI-58 is a CoA-dependent lysophosphatidic acid acyltransferase that channels fatty acids released from the hydrolysis of stored triacylglycerols into phospholipids, overview Mus musculus ?
-
 ?
2.3.1.51 additional information CGI-58 is a CoA-dependent lysophosphatidic acid acyltransferase that channels fatty acids released from the hydrolysis of stored triacylglycerols into phospholipids, overview Homo sapiens ?
-
 ?
2.3.1.51 additional information the CGI-58 carboxyl terminus includes a highly conserved consensus sequence, HXXXXD, required for acyltransferase activity Homo sapiens ?
-
 ?
2.3.1.51 additional information the recombinant CoA-dependent His-tagged CGI-58 is active with lysophosphatidic acid, but not with other lysophospholipid or neutral glycerolipid acceptors Mus musculus ?
-
 ?
2.3.1.51 oleoyl-CoA + 1-arachidonoyl-sn-lysophosphatidylcholine
-
 Mus musculus CoA + 1-arachidonoyl-2-oleoyl-sn-glycero-3-phosphocholine
-
 ?
2.3.1.51 oleoyl-CoA + 1-oleoyl-2-lysophosphatidylcholine
-
 Mus musculus CoA + 1,2-dioleoyl-sn-glycero-3-phosphocholine
-
 ?
2.3.1.51 oleoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate and arachidonoyl-CoA, preferred substrates. Slight preference for 1-oleoyl lysophosphatidic acid over 1-palmitoyl, 1-stearoyl, or 1-arachidonoyl lysophosphatidic acid Mus musculus CoA + 1,2-dioleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.51 oleoyl-CoA + 1-palmitoyl-sn-lysophosphatidylcholine
-
 Mus musculus CoA + 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine
-
 ?
2.3.1.51 oleoyl-CoA + 1-stearoyl-sn-lysophosphatidylcholine
-
 Mus musculus CoA + 1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.51 More the CGI-58 carboxyl terminus includes a highly conserved consensus sequence, HXXXXD, required for acyltransferase activity Mus musculus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.51 ABHD5
-
 Mus musculus
2.3.1.51 CGFI-58
-
 Mus musculus
2.3.1.51 CGI-58/ABHD5
-
 Mus musculus
2.3.1.51 CGI-58/ABHD5
-
 Homo sapiens
2.3.1.51 coenzyme A-dependent lysophosphatidic acid
-
 Mus musculus
2.3.1.51 coenzyme A-dependent lysophosphatidic acid
-
 Homo sapiens
2.3.1.51 More the enzyme belongs to the alpha/beta-hydrolase family Mus musculus
2.3.1.51 More the enzyme belongs to the alpha/beta-hydrolase family Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.51 47
-
 10 min, 60% loss of activity Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.51 7 8.5
-
 Mus musculus

General Information

EC Number General Information Comment Organism
2.3.1.51 malfunction mutations in human CGI-58/ABHD5 cause Chanarin-Dorfman syndrome, characterized by excessive storage of triacylglycerol in tissues Homo sapiens
2.3.1.51 physiological function expression of isoform CGI-58 in fibroblasts from humans with Chanarin-Dorfman increases the incorporation of fatty acids released from the lipolysis of stored triacylglycerols into phospholipids Mus musculus