EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.28 | gene treH is organized in a gene cluster with the other two genes of the trehalose metabolism pathway, treZ and treY encoding malto-oligosyltrehalose trehalohydrolase and maltooligosyltrehalose synthase, DNA and amino acid sequence determination and analysis, overview. Expression of the N-terminally His-tagged active enzyme in Escherichia coli | Nostoc punctiforme |
EC Number | General Stability | Organism |
---|---|---|
3.2.1.141 | active in the presence of Tris | Nostoc punctiforme |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.28 | CaCl2 | over 90% inhibition at 10 mM | Nostoc punctiforme | |
3.2.1.28 | MgCl2 | over 90% inhibition at 10 mM | Nostoc punctiforme | |
3.2.1.28 | MgSO4 | over 90% inhibition at 20 mM | Nostoc punctiforme | |
3.2.1.28 | MnCl2 | over 90% inhibition at 10 mM | Nostoc punctiforme | |
3.2.1.28 | additional information | the enzyme activity is reduced under water-stress | Nostoc punctiforme | |
3.2.1.28 | NaCl | strong inhibition at 10 mM, treatment of cells leads to accumulation of trehalose | Nostoc punctiforme | |
3.2.1.28 | Tris | almost complete inhibition at 5 mM | Nostoc punctiforme |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.28 | 18 | - |
trehalose | pH 7.5, 37°C, recombinant enzyme | Nostoc punctiforme |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.28 | 52000 | - |
x * 52000, recombinant His-tagged active enzyme, SDS-PAGE | Nostoc punctiforme |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.28 | additional information | Nostoc punctiforme | mechanism of trehalose accumulation in response to desiccation and salt stress, overview | ? | - |
? | |
3.2.1.28 | additional information | Nostoc punctiforme IAM M-15 | mechanism of trehalose accumulation in response to desiccation and salt stress, overview | ? | - |
? | |
3.2.1.28 | trehalose + H2O | Nostoc punctiforme | - |
2 D-glucose | - |
? | |
3.2.1.28 | trehalose + H2O | Nostoc punctiforme IAM M-15 | - |
2 D-glucose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.28 | Nostoc punctiforme | B6ZIV1 | gene treH | - |
3.2.1.28 | Nostoc punctiforme IAM M-15 | B6ZIV1 | gene treH | - |
3.2.1.141 | Nostoc punctiforme | - |
- |
- |
3.2.1.141 | Nostoc punctiforme IAM M-15 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.28 | recombinant His-tagged enzyme by His affinity chromatography to homogeneity | Nostoc punctiforme |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.28 | additional information | - |
determination of trehalose contents in differently treated cells, overview | Nostoc punctiforme |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.28 | additional information | mechanism of trehalose accumulation in response to desiccation and salt stress, overview | Nostoc punctiforme | ? | - |
? | |
3.2.1.28 | additional information | mechanism of trehalose accumulation in response to desiccation and salt stress, overview | Nostoc punctiforme IAM M-15 | ? | - |
? | |
3.2.1.28 | trehalose + H2O | - |
Nostoc punctiforme | 2 D-glucose | - |
? | |
3.2.1.28 | trehalose + H2O | - |
Nostoc punctiforme IAM M-15 | 2 D-glucose | - |
? | |
3.2.1.141 | 4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O | - |
Nostoc punctiforme | (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose | - |
? | |
3.2.1.141 | 4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O | - |
Nostoc punctiforme IAM M-15 | (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.28 | ? | x * 52000, recombinant His-tagged active enzyme, SDS-PAGE | Nostoc punctiforme |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.28 | trehalase | - |
Nostoc punctiforme |
3.2.1.141 | maltooligosyltrehalose trehalohydrolase | - |
Nostoc punctiforme |
3.2.1.141 | MTH | - |
Nostoc punctiforme |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.28 | 37 | - |
assay at | Nostoc punctiforme |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.28 | 7.5 | - |
assay at | Nostoc punctiforme |
3.2.1.141 | 7 | - |
- |
Nostoc punctiforme |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.2.1.28 | metabolism | the enzyme catalyzes the last step in the trehalose metabolism and is the key enzyme controlling the pathway in response to water conditions | Nostoc punctiforme |