Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yoshida, T.; Sakamoto, T.
    Water-stress induced trehalose accumulation and control of trehalase in the cyanobacterium Nostoc punctiforme IAM M-15 (2009), J. Gen. Appl. Microbiol., 55, 135-145.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.28 gene treH is organized in a gene cluster with the other two genes of the trehalose metabolism pathway, treZ and treY encoding malto-oligosyltrehalose trehalohydrolase and maltooligosyltrehalose synthase, DNA and amino acid sequence determination and analysis, overview. Expression of the N-terminally His-tagged active enzyme in Escherichia coli Nostoc punctiforme

General Stability

EC Number General Stability Organism
3.2.1.141 active in the presence of Tris Nostoc punctiforme

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.28 CaCl2 over 90% inhibition at 10 mM Nostoc punctiforme
3.2.1.28 MgCl2 over 90% inhibition at 10 mM Nostoc punctiforme
3.2.1.28 MgSO4 over 90% inhibition at 20 mM Nostoc punctiforme
3.2.1.28 MnCl2 over 90% inhibition at 10 mM Nostoc punctiforme
3.2.1.28 additional information the enzyme activity is reduced under water-stress Nostoc punctiforme
3.2.1.28 NaCl strong inhibition at 10 mM, treatment of cells leads to accumulation of trehalose Nostoc punctiforme
3.2.1.28 Tris almost complete inhibition at 5 mM Nostoc punctiforme

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.28 18
-
trehalose pH 7.5, 37°C, recombinant enzyme Nostoc punctiforme

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.28 52000
-
x * 52000, recombinant His-tagged active enzyme, SDS-PAGE Nostoc punctiforme

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.28 additional information Nostoc punctiforme mechanism of trehalose accumulation in response to desiccation and salt stress, overview ?
-
?
3.2.1.28 additional information Nostoc punctiforme IAM M-15 mechanism of trehalose accumulation in response to desiccation and salt stress, overview ?
-
?
3.2.1.28 trehalose + H2O Nostoc punctiforme
-
2 D-glucose
-
?
3.2.1.28 trehalose + H2O Nostoc punctiforme IAM M-15
-
2 D-glucose
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.28 Nostoc punctiforme B6ZIV1 gene treH
-
3.2.1.28 Nostoc punctiforme IAM M-15 B6ZIV1 gene treH
-
3.2.1.141 Nostoc punctiforme
-
-
-
3.2.1.141 Nostoc punctiforme IAM M-15
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.28 recombinant His-tagged enzyme by His affinity chromatography to homogeneity Nostoc punctiforme

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.28 additional information
-
determination of trehalose contents in differently treated cells, overview Nostoc punctiforme

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.28 additional information mechanism of trehalose accumulation in response to desiccation and salt stress, overview Nostoc punctiforme ?
-
?
3.2.1.28 additional information mechanism of trehalose accumulation in response to desiccation and salt stress, overview Nostoc punctiforme IAM M-15 ?
-
?
3.2.1.28 trehalose + H2O
-
Nostoc punctiforme 2 D-glucose
-
?
3.2.1.28 trehalose + H2O
-
Nostoc punctiforme IAM M-15 2 D-glucose
-
?
3.2.1.141 4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O
-
Nostoc punctiforme (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose
-
?
3.2.1.141 4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O
-
Nostoc punctiforme IAM M-15 (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.28 ? x * 52000, recombinant His-tagged active enzyme, SDS-PAGE Nostoc punctiforme

Synonyms

EC Number Synonyms Comment Organism
3.2.1.28 trehalase
-
Nostoc punctiforme
3.2.1.141 maltooligosyltrehalose trehalohydrolase
-
Nostoc punctiforme
3.2.1.141 MTH
-
Nostoc punctiforme

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.28 37
-
assay at Nostoc punctiforme

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.28 7.5
-
assay at Nostoc punctiforme
3.2.1.141 7
-
-
Nostoc punctiforme

General Information

EC Number General Information Comment Organism
3.2.1.28 metabolism the enzyme catalyzes the last step in the trehalose metabolism and is the key enzyme controlling the pathway in response to water conditions Nostoc punctiforme