EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.7.48 | in vitro transcription of NS5B using SP6 RNA polymerase, and recombinant expression of His6-tagged NS5B and of untagged RDRP in Hep-G2 cells. In vitro cleavage of HCV core and NS5B mRNAs by DNAzyme in Hep-G2 cells is assessed by RT-PCR using RNA from cells co-transfected with cloned core or NS5B gene and its respective DNAzyme leading to reduction of NS5B protein activity in NDNAzyme co-transfected cells | Hepacivirus C |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.48 | additional information | reduction of NS5B activity by co-expression of an NS5B mRNA-cleaving DNAzyme in Hep-G2 cells | Hepacivirus C |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.48 | Mn2+ | - |
Hepacivirus C |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.7.48 | 42000 | - |
x * 42000, recombinant His6-tagged NS5B, SDS-PAGE, x * 68000, recombinant RDRP, SDS-PAGE | Hepacivirus C |
2.7.7.48 | 68000 | - |
x * 42000, recombinant His6-tagged NS5B, SDS-PAGE, x * 68000, recombinant RDRP, SDS-PAGE | Hepacivirus C |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.48 | nucleoside triphosphate + RNAn | Hepacivirus C | - |
diphosphate + RNAn+1 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.48 | Hepacivirus C | Q0G9P7 | i.e. HCV | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.48 | recombinant His6-tagged NS5B from Hep-G2 cells by nickel affinity chromatography | Hepacivirus C |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.48 | nucleoside triphosphate + RNAn | - |
Hepacivirus C | diphosphate + RNAn+1 | - |
? | |
2.7.7.48 | nucleoside triphosphate + RNAn | nculeotides are GTP, CTP, ATP, and UTP, the RNA templates for the enzyme assay are transcribed from linearized murine inducible nitric oxide synthase, iNOS, clone having 400 nt insert in an in vitro transcription reaction using SP6 RNA polymerase | Hepacivirus C | diphosphate + RNAn+1 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.7.48 | ? | x * 42000, recombinant His6-tagged NS5B, SDS-PAGE, x * 68000, recombinant RDRP, SDS-PAGE | Hepacivirus C |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.7.48 | NS5B | - |
Hepacivirus C |
2.7.7.48 | RDRP | - |
Hepacivirus C |
2.7.7.48 | RNA dependent RNA polymerase | - |
Hepacivirus C |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.48 | 30 | - |
assay at | Hepacivirus C |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.48 | 8 | - |
assay at | Hepacivirus C |