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Literature summary extracted from

  • Kumar, D.; Chaudhury, I.; Kar, P.; Das, R.H.
    Site-specific cleavage of HCV genomic RNA and its cloned core and NS5B genes by DNAzyme (2009), J. Gastroenterol. Hepatol., 24, 872-878.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.48 in vitro transcription of NS5B using SP6 RNA polymerase, and recombinant expression of His6-tagged NS5B and of untagged RDRP in Hep-G2 cells. In vitro cleavage of HCV core and NS5B mRNAs by DNAzyme in Hep-G2 cells is assessed by RT-PCR using RNA from cells co-transfected with cloned core or NS5B gene and its respective DNAzyme leading to reduction of NS5B protein activity in NDNAzyme co-transfected cells Hepacivirus C

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.48 additional information reduction of NS5B activity by co-expression of an NS5B mRNA-cleaving DNAzyme in Hep-G2 cells Hepacivirus C

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.48 Mn2+
-
Hepacivirus C

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.48 42000
-
x * 42000, recombinant His6-tagged NS5B, SDS-PAGE, x * 68000, recombinant RDRP, SDS-PAGE Hepacivirus C
2.7.7.48 68000
-
x * 42000, recombinant His6-tagged NS5B, SDS-PAGE, x * 68000, recombinant RDRP, SDS-PAGE Hepacivirus C

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn Hepacivirus C
-
diphosphate + RNAn+1
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Hepacivirus C Q0G9P7 i.e. HCV
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.48 recombinant His6-tagged NS5B from Hep-G2 cells by nickel affinity chromatography Hepacivirus C

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn
-
Hepacivirus C diphosphate + RNAn+1
-
?
2.7.7.48 nucleoside triphosphate + RNAn nculeotides are GTP, CTP, ATP, and UTP, the RNA templates for the enzyme assay are transcribed from linearized murine inducible nitric oxide synthase, iNOS, clone having 400 nt insert in an in vitro transcription reaction using SP6 RNA polymerase Hepacivirus C diphosphate + RNAn+1
-
?

Subunits

EC Number Subunits Comment Organism
2.7.7.48 ? x * 42000, recombinant His6-tagged NS5B, SDS-PAGE, x * 68000, recombinant RDRP, SDS-PAGE Hepacivirus C

Synonyms

EC Number Synonyms Comment Organism
2.7.7.48 NS5B
-
Hepacivirus C
2.7.7.48 RDRP
-
Hepacivirus C
2.7.7.48 RNA dependent RNA polymerase
-
Hepacivirus C

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.48 30
-
assay at Hepacivirus C

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.48 8
-
assay at Hepacivirus C