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Literature summary extracted from

  • Yamada, Y.; Fukuda, W.; Hirooka, K.; Hiromoto, T.; Nakayama, J.; Imanaka, T.; Fukusaki, E.; Fujiwara, S.
    Efficient in vitro synthesis of cis-polyisoprenes using a thermostable cis-prenyltransferase from a hyperthermophilic archaeon Thermococcus kodakaraensis (2009), J. Biotechnol., 143, 151-156.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.87
-
 Thermococcus kodakarensis

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.87 E68A product distributions is shifted to longer region to give the C65–C70 as the main products Thermococcus kodakarensis
2.5.1.87 K109A product distributions is shifted to longer region to give the C65–C70 as the main products Thermococcus kodakarensis
2.5.1.87 L113A product distributions is shifted to longer region to give the C65–C70 as the main products Thermococcus kodakarensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.87 0.00096
-
 farnesyl diphosphate 65°C Thermococcus kodakarensis
2.5.1.87 0.00175
-
 geranyl diphosphate 65°C Thermococcus kodakarensis
2.5.1.87 0.00588
-
 isopentenyl diphosphate 65°C, cosubstrate: geranyl diphosphate Thermococcus kodakarensis
2.5.1.87 0.0094
-
 dimethylallyl diphosphate 65°C Thermococcus kodakarensis
2.5.1.87 0.0132
-
 isopentenyl diphosphate 65°C, cosubstrate: farnesyl diphosphate Thermococcus kodakarensis
2.5.1.87 0.111
-
 isopentenyl diphosphate 65°C, cosubstrate: dimethylallyl diphosphate Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.87 (2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate Thermococcus kodakarensis the enzyme preferably utilizes both geranyl diphosphate and farnesyl diphosphate as the starting substrate n diphosphate + di-trans,poly-cis-polyprenyl diphosphate
-
 ?
2.5.1.87 geranyl diphosphate + n isopentenyl diphosphate Thermococcus kodakarensis the enzyme preferably utilizes both geranyl diphosphate and farnesyl diphosphate as the starting substrate n diphosphate + di-trans,poly-cis-polyprenyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.87 Thermococcus kodakarensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.87
-
 Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.87 (2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate the enzyme preferably utilizes both geranyl diphosphate and farnesyl diphosphate as the starting substrate Thermococcus kodakarensis n diphosphate + di-trans,poly-cis-polyprenyl diphosphate
-
 ?
2.5.1.87 dimethylallyl diphosphate + n isopentenyl diphosphate weak activity Thermococcus kodakarensis n diphosphate + di-trans,poly-cis-polyprenyl diphosphate
-
 ?
2.5.1.87 geranyl diphosphate + n isopentenyl diphosphate the enzyme preferably utilizes both geranyl diphosphate and farnesyl diphosphate as the starting substrate Thermococcus kodakarensis n diphosphate + di-trans,poly-cis-polyprenyl diphosphate
-
 ?
2.5.1.87 geranyl diphosphate + n isopentenyl diphosphate the enzyme preferably utilizes both geranyl diphosphate and farnesyl diphosphate as the starting substrate Thermococcus kodakarensis n diphosphate + di-trans,poly-cis-polyprenyl diphosphate Tk-IdsB produces cis-polyprenyl diphosphates ranging from C15 to C90. Above C75: 11.5%. C65–C70: 60.7%. C55–C60: 17.4%. C45–C50: 3.3%. C30–C40: 3.9%. Below C25: 3.2%, mainly yields the C60–C65 products ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.87 Tk-IdsB
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.87 60 70 pH 8.5 Thermococcus kodakarensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.87 80
-
 half-life: 46 min, half-life is prolonged by immobilization on silica beads to 156 min Thermococcus kodakarensis
2.5.1.87 96
-
 not completely denatured Thermococcus kodakarensis