EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.6 | His-tagged, expressed in Escherichia coli BL21(DE3) | Rattus norvegicus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.6 | D186A | residue D186 interacts with galactose, possible catalytic residue, no activity of mutant enzyme | Rattus norvegicus |
2.7.1.6 | D186E | residue D186 interacts with galactose, possible catalytic residue, no activity of mutant enzyme | Rattus norvegicus |
2.7.1.6 | D186N | residue D186 interacts with galactose, possible catalytic residue, no activity of mutant enzyme | Rattus norvegicus |
2.7.1.6 | R37A | residue R37 interacts with galactose | Rattus norvegicus |
2.7.1.6 | Y109F | residue Y109interacts with ATP | Rattus norvegicus |
2.7.1.6 | Y109H | residue Y109interacts with ATP | Rattus norvegicus |
2.7.1.6 | Y236A | residue Y236 interacts with galactose | Rattus norvegicus |
2.7.1.6 | Y236F | residue Y236 interacts with galactose | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.6 | amikacin | - |
Rattus norvegicus | |
2.7.1.6 | AMP | - |
Rattus norvegicus | |
2.7.1.6 | bromo alpha-D-galactopyranoside | - |
Rattus norvegicus | |
2.7.1.6 | fluoro alpha-D-galactopyranoside | - |
Rattus norvegicus | |
2.7.1.6 | galactose-1-phosphate | - |
Rattus norvegicus | |
2.7.1.6 | genistein | - |
Rattus norvegicus | |
2.7.1.6 | gentamycin | - |
Rattus norvegicus | |
2.7.1.6 | kanamycin A | - |
Rattus norvegicus | |
2.7.1.6 | neomycin | - |
Rattus norvegicus | |
2.7.1.6 | paromycin | - |
Rattus norvegicus | |
2.7.1.6 | quercetin | - |
Rattus norvegicus | |
2.7.1.6 | tobramycin | - |
Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.6 | 0.0948 | - |
ATP | wild-type protein | Rattus norvegicus | |
2.7.1.6 | 0.136 | - |
ATP | Y236F mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.152 | - |
ATP | R37A mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.698 | - |
alpha-D-galactose | wild-type protein | Rattus norvegicus | |
2.7.1.6 | 0.755 | - |
ATP | Y109F mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.784 | - |
alpha-D-galactose | R37A mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.823 | - |
alpha-D-galactose | Y109F mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.986 | - |
alpha-D-galactose | Y236F mutant protein | Rattus norvegicus | |
2.7.1.6 | 1.118 | - |
alpha-D-galactose | Y109H mutant protein | Rattus norvegicus | |
2.7.1.6 | 3.83 | - |
ATP | Y109H mutant protein | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.6 | 43000 | - |
x * 43000 Da, SDS-PAGE | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.6 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.6 | - |
Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.1.6 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.6 | 0.07 | - |
R37A mutant protein, substrate: galactose | Rattus norvegicus |
2.7.1.6 | 0.14 | - |
Y109F mutant protein, substrate: galactose | Rattus norvegicus |
2.7.1.6 | 0.17 | - |
Y236F mutant protein, substrate: galactose | Rattus norvegicus |
2.7.1.6 | 0.91 | - |
wild-type protein, substrate: galactose | Rattus norvegicus |
2.7.1.6 | 1.14 | - |
Y109H mutant protein, substrate: galactose | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.6 | 2-amino-2-deoxy-D-galactose + ATP | - |
Rattus norvegicus | 2-amino-2-deoxy-D-galactose 1-phosphate + ADP | - |
? | |
2.7.1.6 | 2-deoxy-D-galactose + ATP | - |
Rattus norvegicus | 2-deoxy-D-galactose 1-phosphate + ADP | - |
? | |
2.7.1.6 | 3-deoxy-D-galactose + ATP | - |
Rattus norvegicus | ? | - |
? | |
2.7.1.6 | alpha-D-galactose + ATP | - |
Rattus norvegicus | alpha-D-galactose 1-phosphate + ADP | - |
? | |
2.7.1.6 | D-fucose + ATP | - |
Rattus norvegicus | ? | - |
? | |
2.7.1.6 | D-galactosamine + ATP | - |
Rattus norvegicus | ? | - |
? | |
2.7.1.6 | D-talose + ATP | - |
Rattus norvegicus | alpha-D-talose 1-phosphate + ADP | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.6 | ? | x * 43000 Da, SDS-PAGE | Rattus norvegicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.6 | galactokinase | - |
Rattus norvegicus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.6 | 0.05 | - |
alpha-D-galactose | R37A mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.1 | - |
alpha-D-galactose | Y109F mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.1 | - |
alpha-D-galactose | Y109H mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.12 | - |
alpha-D-galactose | Y236F mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.65 | - |
alpha-D-galactose | wild-type protein | Rattus norvegicus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.6 | 0.0225 | - |
quercetin | - |
Rattus norvegicus | |
2.7.1.6 | 0.0739 | - |
genistein | - |
Rattus norvegicus | |
2.7.1.6 | 10.2 | - |
AMP | - |
Rattus norvegicus |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
2.7.1.6 | 0.86 | - |
- |
Rattus norvegicus | fluoro alpha-D-galactopyranoside | |
2.7.1.6 | 4.4 | - |
- |
Rattus norvegicus | bromo alpha-D-galactopyranoside | |
2.7.1.6 | 7.2 | - |
- |
Rattus norvegicus | tobramycin | |
2.7.1.6 | 16.8 | - |
- |
Rattus norvegicus | Galactose 1-phosphate | |
2.7.1.6 | 20 | - |
- |
Rattus norvegicus | kanamycin A | |
2.7.1.6 | 30 | - |
- |
Rattus norvegicus | neomycin | |
2.7.1.6 | 36.6 | - |
- |
Rattus norvegicus | gentamycin | |
2.7.1.6 | 49.9 | - |
- |
Rattus norvegicus | amikacin | |
2.7.1.6 | 67.3 | - |
- |
Rattus norvegicus | paromycin |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.6 | 0.00057 | - |
D-fucose | Y236F mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.00062 | - |
D-fucose | R37A mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.0022 | - |
2-amino-2-deoxy-D-galactose | R37A mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.0027 | - |
2-amino-2-deoxy-D-galactose | Y236F mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.0047 | - |
D-fucose | wild-type protein | Rattus norvegicus | |
2.7.1.6 | 0.0052 | - |
D-talose | R37A mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.012 | - |
D-fucose | Y109F mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.013 | - |
D-galactosamine | Y236F mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.019 | - |
2-deoxy-D-galactose | R37A mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.019 | - |
D-talose | Y109H mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.024 | - |
3-Deoxy-D-galactose | Y109F mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.026 | - |
2-amino-2-deoxy-D-galactose | Y109F mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.026 | - |
D-fucose | Y109H mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.026 | - |
2-deoxy-D-galactose | Y109H mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.028 | - |
3-Deoxy-D-galactose | Y109H mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.031 | - |
D-talose | Y109F mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.032 | - |
2-amino-2-deoxy-D-galactose | wild-type protein | Rattus norvegicus | |
2.7.1.6 | 0.037 | - |
3-Deoxy-D-galactose | R37A mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.037 | - |
2-deoxy-D-galactose | Y109F mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.041 | - |
3-Deoxy-D-galactose | Y236F mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.047 | - |
D-galactosamine | Y109H mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.047 | - |
2-deoxy-D-galactose | Y236F mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.054 | - |
3-Deoxy-D-galactose | wild-type protein | Rattus norvegicus | |
2.7.1.6 | 0.057 | - |
D-talose | Y236F mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.059 | - |
2-amino-2-deoxy-D-galactose | Y109H mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.06 | - |
D-galactosamine | Y109F mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.064 | - |
alpha-D-galactose | R37A mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.066 | - |
D-galactosamine | R37A mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.089 | - |
alpha-D-galactose | Y109H mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.12 | - |
alpha-D-galactose | Y109F mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.122 | - |
alpha-D-galactose | Y236F mutant protein | Rattus norvegicus | |
2.7.1.6 | 0.14 | - |
D-talose | wild-type protein | Rattus norvegicus | |
2.7.1.6 | 0.237 | - |
2-deoxy-D-galactose | wild-type protein | Rattus norvegicus | |
2.7.1.6 | 0.615 | - |
D-galactosamine | wild-type protein | Rattus norvegicus | |
2.7.1.6 | 0.93 | - |
alpha-D-galactose | wild-type protein | Rattus norvegicus |