BRENDA - Enzyme Database

Functional studies of rat galactokinase

Chu, X.; Li, N.; Liu, X.; Li, D.; J. Biotechnol. 141, 142-146 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.6
His-tagged, expressed in Escherichia coli BL21(DE3)
Rattus norvegicus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.7.1.6
D186A
residue D186 interacts with galactose, possible catalytic residue, no activity of mutant enzyme
Rattus norvegicus
2.7.1.6
D186E
residue D186 interacts with galactose, possible catalytic residue, no activity of mutant enzyme
Rattus norvegicus
2.7.1.6
D186N
residue D186 interacts with galactose, possible catalytic residue, no activity of mutant enzyme
Rattus norvegicus
2.7.1.6
R37A
residue R37 interacts with galactose
Rattus norvegicus
2.7.1.6
Y109F
residue Y109interacts with ATP
Rattus norvegicus
2.7.1.6
Y109H
residue Y109interacts with ATP
Rattus norvegicus
2.7.1.6
Y236A
residue Y236 interacts with galactose
Rattus norvegicus
2.7.1.6
Y236F
residue Y236 interacts with galactose
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.6
amikacin
-
Rattus norvegicus
2.7.1.6
AMP
-
Rattus norvegicus
2.7.1.6
bromo alpha-D-galactopyranoside
-
Rattus norvegicus
2.7.1.6
fluoro alpha-D-galactopyranoside
-
Rattus norvegicus
2.7.1.6
galactose-1-phosphate
-
Rattus norvegicus
2.7.1.6
genistein
-
Rattus norvegicus
2.7.1.6
gentamycin
-
Rattus norvegicus
2.7.1.6
kanamycin A
-
Rattus norvegicus
2.7.1.6
neomycin
-
Rattus norvegicus
2.7.1.6
paromycin
-
Rattus norvegicus
2.7.1.6
quercetin
-
Rattus norvegicus
2.7.1.6
tobramycin
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.6
0.0948
-
ATP
wild-type protein
Rattus norvegicus
2.7.1.6
0.136
-
ATP
Y236F mutant protein
Rattus norvegicus
2.7.1.6
0.152
-
ATP
R37A mutant protein
Rattus norvegicus
2.7.1.6
0.698
-
alpha-D-galactose
wild-type protein
Rattus norvegicus
2.7.1.6
0.755
-
ATP
Y109F mutant protein
Rattus norvegicus
2.7.1.6
0.784
-
alpha-D-galactose
R37A mutant protein
Rattus norvegicus
2.7.1.6
0.823
-
alpha-D-galactose
Y109F mutant protein
Rattus norvegicus
2.7.1.6
0.986
-
alpha-D-galactose
Y236F mutant protein
Rattus norvegicus
2.7.1.6
1.118
-
alpha-D-galactose
Y109H mutant protein
Rattus norvegicus
2.7.1.6
3.83
-
ATP
Y109H mutant protein
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.6
43000
-
x * 43000 Da, SDS-PAGE
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.6
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.6
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.6
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.6
0.07
-
R37A mutant protein, substrate: galactose
Rattus norvegicus
2.7.1.6
0.14
-
Y109F mutant protein, substrate: galactose
Rattus norvegicus
2.7.1.6
0.17
-
Y236F mutant protein, substrate: galactose
Rattus norvegicus
2.7.1.6
0.91
-
wild-type protein, substrate: galactose
Rattus norvegicus
2.7.1.6
1.14
-
Y109H mutant protein, substrate: galactose
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.6
2-amino-2-deoxy-D-galactose + ATP
-
704761
Rattus norvegicus
2-amino-2-deoxy-D-galactose 1-phosphate + ADP
-
-
-
?
2.7.1.6
2-deoxy-D-galactose + ATP
-
704761
Rattus norvegicus
2-deoxy-D-galactose 1-phosphate + ADP
-
-
-
?
2.7.1.6
3-deoxy-D-galactose + ATP
-
704761
Rattus norvegicus
?
-
-
-
?
2.7.1.6
alpha-D-galactose + ATP
-
704761
Rattus norvegicus
alpha-D-galactose 1-phosphate + ADP
-
-
-
?
2.7.1.6
D-fucose + ATP
-
704761
Rattus norvegicus
?
-
-
-
?
2.7.1.6
D-galactosamine + ATP
-
704761
Rattus norvegicus
?
-
-
-
?
2.7.1.6
D-talose + ATP
-
704761
Rattus norvegicus
alpha-D-talose 1-phosphate + ADP
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.6
?
x * 43000 Da, SDS-PAGE
Rattus norvegicus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.6
0.05
-
alpha-D-galactose
R37A mutant protein
Rattus norvegicus
2.7.1.6
0.1
-
alpha-D-galactose
Y109F mutant protein; Y109H mutant protein
Rattus norvegicus
2.7.1.6
0.12
-
alpha-D-galactose
Y236F mutant protein
Rattus norvegicus
2.7.1.6
0.65
-
alpha-D-galactose
wild-type protein
Rattus norvegicus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.6
0.0225
-
quercetin
-
Rattus norvegicus
2.7.1.6
0.0739
-
genistein
-
Rattus norvegicus
2.7.1.6
10.2
-
AMP
-
Rattus norvegicus
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
2.7.1.6
0.86
-
-
Rattus norvegicus
fluoro alpha-D-galactopyranoside
2.7.1.6
4.4
-
-
Rattus norvegicus
bromo alpha-D-galactopyranoside
2.7.1.6
7.2
-
-
Rattus norvegicus
tobramycin
2.7.1.6
16.8
-
-
Rattus norvegicus
Galactose 1-phosphate
2.7.1.6
20
-
-
Rattus norvegicus
kanamycin A
2.7.1.6
30
-
-
Rattus norvegicus
neomycin
2.7.1.6
36.6
-
-
Rattus norvegicus
gentamycin
2.7.1.6
49.9
-
-
Rattus norvegicus
amikacin
2.7.1.6
67.3
-
-
Rattus norvegicus
paromycin
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.6
His-tagged, expressed in Escherichia coli BL21(DE3)
Rattus norvegicus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.7.1.6
D186A
residue D186 interacts with galactose, possible catalytic residue, no activity of mutant enzyme
Rattus norvegicus
2.7.1.6
D186E
residue D186 interacts with galactose, possible catalytic residue, no activity of mutant enzyme
Rattus norvegicus
2.7.1.6
D186N
residue D186 interacts with galactose, possible catalytic residue, no activity of mutant enzyme
Rattus norvegicus
2.7.1.6
R37A
residue R37 interacts with galactose
Rattus norvegicus
2.7.1.6
Y109F
residue Y109interacts with ATP
Rattus norvegicus
2.7.1.6
Y109H
residue Y109interacts with ATP
Rattus norvegicus
2.7.1.6
Y236A
residue Y236 interacts with galactose
Rattus norvegicus
2.7.1.6
Y236F
residue Y236 interacts with galactose
Rattus norvegicus
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
2.7.1.6
0.86
-
-
Rattus norvegicus
fluoro alpha-D-galactopyranoside
2.7.1.6
4.4
-
-
Rattus norvegicus
bromo alpha-D-galactopyranoside
2.7.1.6
7.2
-
-
Rattus norvegicus
tobramycin
2.7.1.6
16.8
-
-
Rattus norvegicus
Galactose 1-phosphate
2.7.1.6
20
-
-
Rattus norvegicus
kanamycin A
2.7.1.6
30
-
-
Rattus norvegicus
neomycin
2.7.1.6
36.6
-
-
Rattus norvegicus
gentamycin
2.7.1.6
49.9
-
-
Rattus norvegicus
amikacin
2.7.1.6
67.3
-
-
Rattus norvegicus
paromycin
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.6
amikacin
-
Rattus norvegicus
2.7.1.6
AMP
-
Rattus norvegicus
2.7.1.6
bromo alpha-D-galactopyranoside
-
Rattus norvegicus
2.7.1.6
fluoro alpha-D-galactopyranoside
-
Rattus norvegicus
2.7.1.6
galactose-1-phosphate
-
Rattus norvegicus
2.7.1.6
genistein
-
Rattus norvegicus
2.7.1.6
gentamycin
-
Rattus norvegicus
2.7.1.6
kanamycin A
-
Rattus norvegicus
2.7.1.6
neomycin
-
Rattus norvegicus
2.7.1.6
paromycin
-
Rattus norvegicus
2.7.1.6
quercetin
-
Rattus norvegicus
2.7.1.6
tobramycin
-
Rattus norvegicus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.1.6
0.0225
-
quercetin
-
Rattus norvegicus
2.7.1.6
0.0739
-
genistein
-
Rattus norvegicus
2.7.1.6
10.2
-
AMP
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.6
0.0948
-
ATP
wild-type protein
Rattus norvegicus
2.7.1.6
0.136
-
ATP
Y236F mutant protein
Rattus norvegicus
2.7.1.6
0.152
-
ATP
R37A mutant protein
Rattus norvegicus
2.7.1.6
0.698
-
alpha-D-galactose
wild-type protein
Rattus norvegicus
2.7.1.6
0.755
-
ATP
Y109F mutant protein
Rattus norvegicus
2.7.1.6
0.784
-
alpha-D-galactose
R37A mutant protein
Rattus norvegicus
2.7.1.6
0.823
-
alpha-D-galactose
Y109F mutant protein
Rattus norvegicus
2.7.1.6
0.986
-
alpha-D-galactose
Y236F mutant protein
Rattus norvegicus
2.7.1.6
1.118
-
alpha-D-galactose
Y109H mutant protein
Rattus norvegicus
2.7.1.6
3.83
-
ATP
Y109H mutant protein
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.6
43000
-
x * 43000 Da, SDS-PAGE
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.6
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.6
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.6
0.07
-
R37A mutant protein, substrate: galactose
Rattus norvegicus
2.7.1.6
0.14
-
Y109F mutant protein, substrate: galactose
Rattus norvegicus
2.7.1.6
0.17
-
Y236F mutant protein, substrate: galactose
Rattus norvegicus
2.7.1.6
0.91
-
wild-type protein, substrate: galactose
Rattus norvegicus
2.7.1.6
1.14
-
Y109H mutant protein, substrate: galactose
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.6
2-amino-2-deoxy-D-galactose + ATP
-
704761
Rattus norvegicus
2-amino-2-deoxy-D-galactose 1-phosphate + ADP
-
-
-
?
2.7.1.6
2-deoxy-D-galactose + ATP
-
704761
Rattus norvegicus
2-deoxy-D-galactose 1-phosphate + ADP
-
-
-
?
2.7.1.6
3-deoxy-D-galactose + ATP
-
704761
Rattus norvegicus
?
-
-
-
?
2.7.1.6
alpha-D-galactose + ATP
-
704761
Rattus norvegicus
alpha-D-galactose 1-phosphate + ADP
-
-
-
?
2.7.1.6
D-fucose + ATP
-
704761
Rattus norvegicus
?
-
-
-
?
2.7.1.6
D-galactosamine + ATP
-
704761
Rattus norvegicus
?
-
-
-
?
2.7.1.6
D-talose + ATP
-
704761
Rattus norvegicus
alpha-D-talose 1-phosphate + ADP
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.6
?
x * 43000 Da, SDS-PAGE
Rattus norvegicus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.6
0.05
-
alpha-D-galactose
R37A mutant protein
Rattus norvegicus
2.7.1.6
0.1
-
alpha-D-galactose
Y109F mutant protein; Y109H mutant protein
Rattus norvegicus
2.7.1.6
0.12
-
alpha-D-galactose
Y236F mutant protein
Rattus norvegicus
2.7.1.6
0.65
-
alpha-D-galactose
wild-type protein
Rattus norvegicus
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.7.1.6
0.00057
-
D-fucose
Y236F mutant protein
Rattus norvegicus
2.7.1.6
0.00062
-
D-fucose
R37A mutant protein
Rattus norvegicus
2.7.1.6
0.0022
-
2-amino-2-deoxy-D-galactose
R37A mutant protein
Rattus norvegicus
2.7.1.6
0.0027
-
2-amino-2-deoxy-D-galactose
Y236F mutant protein
Rattus norvegicus
2.7.1.6
0.0047
-
D-fucose
wild-type protein
Rattus norvegicus
2.7.1.6
0.0052
-
D-talose
R37A mutant protein
Rattus norvegicus
2.7.1.6
0.012
-
D-fucose
Y109F mutant protein
Rattus norvegicus
2.7.1.6
0.013
-
D-galactosamine
Y236F mutant protein
Rattus norvegicus
2.7.1.6
0.019
-
2-deoxy-D-galactose
R37A mutant protein
Rattus norvegicus
2.7.1.6
0.019
-
D-talose
Y109H mutant protein
Rattus norvegicus
2.7.1.6
0.024
-
3-Deoxy-D-galactose
Y109F mutant protein
Rattus norvegicus
2.7.1.6
0.026
-
2-amino-2-deoxy-D-galactose
Y109F mutant protein
Rattus norvegicus
2.7.1.6
0.026
-
2-deoxy-D-galactose
Y109H mutant protein
Rattus norvegicus
2.7.1.6
0.026
-
D-fucose
Y109H mutant protein
Rattus norvegicus
2.7.1.6
0.028
-
3-Deoxy-D-galactose
Y109H mutant protein
Rattus norvegicus
2.7.1.6
0.031
-
D-talose
Y109F mutant protein
Rattus norvegicus
2.7.1.6
0.032
-
2-amino-2-deoxy-D-galactose
wild-type protein
Rattus norvegicus
2.7.1.6
0.037
-
2-deoxy-D-galactose
Y109F mutant protein
Rattus norvegicus
2.7.1.6
0.037
-
3-Deoxy-D-galactose
R37A mutant protein
Rattus norvegicus
2.7.1.6
0.041
-
3-Deoxy-D-galactose
Y236F mutant protein
Rattus norvegicus
2.7.1.6
0.047
-
2-deoxy-D-galactose
Y236F mutant protein
Rattus norvegicus
2.7.1.6
0.047
-
D-galactosamine
Y109H mutant protein
Rattus norvegicus
2.7.1.6
0.054
-
3-Deoxy-D-galactose
wild-type protein
Rattus norvegicus
2.7.1.6
0.057
-
D-talose
Y236F mutant protein
Rattus norvegicus
2.7.1.6
0.059
-
2-amino-2-deoxy-D-galactose
Y109H mutant protein
Rattus norvegicus
2.7.1.6
0.06
-
D-galactosamine
Y109F mutant protein
Rattus norvegicus
2.7.1.6
0.064
-
alpha-D-galactose
R37A mutant protein
Rattus norvegicus
2.7.1.6
0.066
-
D-galactosamine
R37A mutant protein
Rattus norvegicus
2.7.1.6
0.089
-
alpha-D-galactose
Y109H mutant protein
Rattus norvegicus
2.7.1.6
0.12
-
alpha-D-galactose
Y109F mutant protein
Rattus norvegicus
2.7.1.6
0.122
-
alpha-D-galactose
Y236F mutant protein
Rattus norvegicus
2.7.1.6
0.14
-
D-talose
wild-type protein
Rattus norvegicus
2.7.1.6
0.237
-
2-deoxy-D-galactose
wild-type protein
Rattus norvegicus
2.7.1.6
0.615
-
D-galactosamine
wild-type protein
Rattus norvegicus
2.7.1.6
0.93
-
alpha-D-galactose
wild-type protein
Rattus norvegicus
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.7.1.6
0.00057
-
D-fucose
Y236F mutant protein
Rattus norvegicus
2.7.1.6
0.00062
-
D-fucose
R37A mutant protein
Rattus norvegicus
2.7.1.6
0.0022
-
2-amino-2-deoxy-D-galactose
R37A mutant protein
Rattus norvegicus
2.7.1.6
0.0027
-
2-amino-2-deoxy-D-galactose
Y236F mutant protein
Rattus norvegicus
2.7.1.6
0.0047
-
D-fucose
wild-type protein
Rattus norvegicus
2.7.1.6
0.0052
-
D-talose
R37A mutant protein
Rattus norvegicus
2.7.1.6
0.012
-
D-fucose
Y109F mutant protein
Rattus norvegicus
2.7.1.6
0.013
-
D-galactosamine
Y236F mutant protein
Rattus norvegicus
2.7.1.6
0.019
-
2-deoxy-D-galactose
R37A mutant protein
Rattus norvegicus
2.7.1.6
0.019
-
D-talose
Y109H mutant protein
Rattus norvegicus
2.7.1.6
0.024
-
3-Deoxy-D-galactose
Y109F mutant protein
Rattus norvegicus
2.7.1.6
0.026
-
2-amino-2-deoxy-D-galactose
Y109F mutant protein
Rattus norvegicus
2.7.1.6
0.026
-
2-deoxy-D-galactose
Y109H mutant protein
Rattus norvegicus
2.7.1.6
0.026
-
D-fucose
Y109H mutant protein
Rattus norvegicus
2.7.1.6
0.028
-
3-Deoxy-D-galactose
Y109H mutant protein
Rattus norvegicus
2.7.1.6
0.031
-
D-talose
Y109F mutant protein
Rattus norvegicus
2.7.1.6
0.032
-
2-amino-2-deoxy-D-galactose
wild-type protein
Rattus norvegicus
2.7.1.6
0.037
-
2-deoxy-D-galactose
Y109F mutant protein
Rattus norvegicus
2.7.1.6
0.037
-
3-Deoxy-D-galactose
R37A mutant protein
Rattus norvegicus
2.7.1.6
0.041
-
3-Deoxy-D-galactose
Y236F mutant protein
Rattus norvegicus
2.7.1.6
0.047
-
2-deoxy-D-galactose
Y236F mutant protein
Rattus norvegicus
2.7.1.6
0.047
-
D-galactosamine
Y109H mutant protein
Rattus norvegicus
2.7.1.6
0.054
-
3-Deoxy-D-galactose
wild-type protein
Rattus norvegicus
2.7.1.6
0.057
-
D-talose
Y236F mutant protein
Rattus norvegicus
2.7.1.6
0.059
-
2-amino-2-deoxy-D-galactose
Y109H mutant protein
Rattus norvegicus
2.7.1.6
0.06
-
D-galactosamine
Y109F mutant protein
Rattus norvegicus
2.7.1.6
0.064
-
alpha-D-galactose
R37A mutant protein
Rattus norvegicus
2.7.1.6
0.066
-
D-galactosamine
R37A mutant protein
Rattus norvegicus
2.7.1.6
0.089
-
alpha-D-galactose
Y109H mutant protein
Rattus norvegicus
2.7.1.6
0.12
-
alpha-D-galactose
Y109F mutant protein
Rattus norvegicus
2.7.1.6
0.122
-
alpha-D-galactose
Y236F mutant protein
Rattus norvegicus
2.7.1.6
0.14
-
D-talose
wild-type protein
Rattus norvegicus
2.7.1.6
0.237
-
2-deoxy-D-galactose
wild-type protein
Rattus norvegicus
2.7.1.6
0.615
-
D-galactosamine
wild-type protein
Rattus norvegicus
2.7.1.6
0.93
-
alpha-D-galactose
wild-type protein
Rattus norvegicus