EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 0.44 | - |
4-nitrophenyl-beta-D-fucopyranoside | in 50 mM citrate buffer, at pH 5.5 and 70°C | Caldicellulosiruptor saccharolyticus | |
3.2.1.21 | 0.67 | - |
4-nitrophenyl-beta-D-glucopyranoside | in 50 mM citrate buffer, at pH 5.5 and 70°C | Caldicellulosiruptor saccharolyticus | |
3.2.1.21 | 1.93 | - |
4-nitrophenyl-beta-D-galactopyranoside | in 50 mM citrate buffer, at pH 5.5 and 70°C | Caldicellulosiruptor saccharolyticus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 54000 | - |
2 * 54000, SDS-PAGE | Caldicellulosiruptor saccharolyticus |
3.2.1.21 | 108000 | - |
gel filtration | Caldicellulosiruptor saccharolyticus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.21 | Caldicellulosiruptor saccharolyticus | A4XIG7 | strain DSM 8903 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.21 | heat treatment and His-Trap affinity chromatography | Caldicellulosiruptor saccharolyticus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 0.08 | - |
crude extract, at 70°C | Caldicellulosiruptor saccharolyticus |
3.2.1.21 | 0.28 | - |
after 163fold purification, using 2-nitrophenyl-beta-D-galactopyranoside as substrate, at 70°C | Caldicellulosiruptor saccharolyticus |
3.2.1.21 | 0.3 | - |
after 163fold purification, using 2-nitrophenyl-beta-D-glucopyranoside as substrate, at 70°C | Caldicellulosiruptor saccharolyticus |
3.2.1.21 | 0.32 | - |
after 163fold purification, using 4-nitrophenyl-beta-D-xylopyranoside as substrate, at 70°C | Caldicellulosiruptor saccharolyticus |
3.2.1.21 | 0.75 | - |
after 163fold purification, using 4-nitrophenyl-beta-D-lactopyranoside as substrate, at 70°C | Caldicellulosiruptor saccharolyticus |
3.2.1.21 | 1.3 | - |
after 163fold purification, using 4-nitrophenyl-beta-D-cellobioside as substrate, at 70°C | Caldicellulosiruptor saccharolyticus |
3.2.1.21 | 6.1 | - |
after 163fold purification, using 4-nitrophenyl-beta-D-galactopyranoside as substrate, at 70°C | Caldicellulosiruptor saccharolyticus |
3.2.1.21 | 6.8 | - |
after 163fold purification, using cellopentaose as substrate, at 70°C | Caldicellulosiruptor saccharolyticus |
3.2.1.21 | 9.5 | - |
after 163fold purification, using cellotriose as substrate, at 70°C | Caldicellulosiruptor saccharolyticus |
3.2.1.21 | 10.8 | - |
after 163fold purification, using cellotetraose as substrate, at 70°C | Caldicellulosiruptor saccharolyticus |
3.2.1.21 | 13 | - |
after 163fold purification, using 4-nitrophenyl-beta-D-glucopyranoside as substrate, at 70°C | Caldicellulosiruptor saccharolyticus |
3.2.1.21 | 14.7 | - |
after 163fold purification, using 4-nitrophenyl-beta-D-fucopyranoside as substrate, at 70°C | Caldicellulosiruptor saccharolyticus |
3.2.1.21 | 16.2 | - |
after 163fold purification, using cellobiose as substrate, at 70°C | Caldicellulosiruptor saccharolyticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.21 | 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Caldicellulosiruptor saccharolyticus | 2-nitrophenol + beta-D-galactose | - |
? | |
3.2.1.21 | 2-nitrophenyl beta-D-glucopyranoside + H2O | - |
Caldicellulosiruptor saccharolyticus | 2-nitrophenol + D-glucopyranose | - |
? | |
3.2.1.21 | 4-nitrophenyl beta-D-cellobioside + H2O | - |
Caldicellulosiruptor saccharolyticus | 4-nitrophenol + cellobiose | - |
? | |
3.2.1.21 | 4-nitrophenyl beta-D-fucopyranoside + H2O | - |
Caldicellulosiruptor saccharolyticus | 4-nitrophenol + beta-D-fucose | - |
? | |
3.2.1.21 | 4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Caldicellulosiruptor saccharolyticus | 4-nitrophenol + beta-D-galactose | - |
? | |
3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Caldicellulosiruptor saccharolyticus | 4-nitrophenol + D-glucopyranose | - |
? | |
3.2.1.21 | 4-nitrophenyl beta-D-lactopyranoside + H2O | - |
Caldicellulosiruptor saccharolyticus | 4-nitrophenol + beta-D-lactose | - |
? | |
3.2.1.21 | 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Caldicellulosiruptor saccharolyticus | 4-nitrophenol + beta-D-xylose | - |
? | |
3.2.1.21 | cellobiose + H2O | - |
Caldicellulosiruptor saccharolyticus | 2 beta-D-glucose | - |
? | |
3.2.1.21 | cellobiose + H2O | highest specific activity | Caldicellulosiruptor saccharolyticus | ? | - |
? | |
3.2.1.21 | cellopentaose + H2O | - |
Caldicellulosiruptor saccharolyticus | ? | - |
? | |
3.2.1.21 | cellotetraose + H2O | - |
Caldicellulosiruptor saccharolyticus | ? | - |
? | |
3.2.1.21 | cellotriose + H2O | - |
Caldicellulosiruptor saccharolyticus | ? | - |
? | |
3.2.1.21 | additional information | cello-oligosaccharides from n equal 2 to 5 as substrates using 4 mM each sugar and 3 units/mg of enzyme are completely hydrolyzed to beta-D-glucose at 70°C within 16 h | Caldicellulosiruptor saccharolyticus | ? | - |
? | |
3.2.1.21 | additional information | the enzyme does not hydrolyze 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-alpha-L-arabinopyranoside, 4-nitrophenyl-beta-D-maltopyranoside, 4-nitrophenyl-beta-D-mannopyranoside, and 4-nitrophenyl-beta-L-arabinopyranoside | Caldicellulosiruptor saccharolyticus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.21 | homodimer | 2 * 54000, SDS-PAGE | Caldicellulosiruptor saccharolyticus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 70 | - |
- |
Caldicellulosiruptor saccharolyticus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 60 | 80 | the half-lives of the enzyme at 60, 70, and 80°C are 250, 24.3, and 0.4 h | Caldicellulosiruptor saccharolyticus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 82 | - |
4-nitrophenyl-beta-D-galactopyranoside | in 50 mM citrate buffer, at pH 5.5 and 70°C | Caldicellulosiruptor saccharolyticus | |
3.2.1.21 | 387 | - |
4-nitrophenyl-beta-D-glucopyranoside | in 50 mM citrate buffer, at pH 5.5 and 70°C | Caldicellulosiruptor saccharolyticus | |
3.2.1.21 | 516 | - |
4-nitrophenyl-beta-D-fucopyranoside | in 50 mM citrate buffer, at pH 5.5 and 70°C | Caldicellulosiruptor saccharolyticus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 5.5 | - |
- |
Caldicellulosiruptor saccharolyticus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 42 | - |
4-nitrophenyl-beta-D-galactopyranoside | in 50 mM citrate buffer, at pH 5.5 and 70°C | Caldicellulosiruptor saccharolyticus | |
3.2.1.21 | 579 | - |
4-nitrophenyl-beta-D-glucopyranoside | in 50 mM citrate buffer, at pH 5.5 and 70°C | Caldicellulosiruptor saccharolyticus | |
3.2.1.21 | 1185 | - |
4-nitrophenyl-beta-D-fucopyranoside | in 50 mM citrate buffer, at pH 5.5 and 70°C | Caldicellulosiruptor saccharolyticus |