BRENDA - Enzyme Database show

Importance of membrane structural integrity for RPE65 retinoid isomerization activity

Golczak, M.; Kiser, P.D.; Lodowski, D.T.; Maeda, A.; Palczewski, K.; J. Biol. Chem. 285, 9667-9682 (2010)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.64
additional information
the lipid membrane structure is critical for RPE65 enzymatic activity
Homo sapiens
Crystallization (Commentary)
EC Number
Crystallization
Organism
3.1.1.64
hanging drop vapor diffusion method, using 100 mM MES, pH 6.0, 30% (w/v) polyethylene glycol 200, and 2 mM dithiothreitol, at 22°C
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.64
membrane
microsome
Homo sapiens
16020
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.64
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.64
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.64
retinal pigment epithelium
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.64
all-trans-retinyl ester + H2O
-
704711
Homo sapiens
11-cis-retinol + a fatty acid
-
-
-
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.64
additional information
the lipid membrane structure is critical for RPE65 enzymatic activity
Homo sapiens
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
3.1.1.64
hanging drop vapor diffusion method, using 100 mM MES, pH 6.0, 30% (w/v) polyethylene glycol 200, and 2 mM dithiothreitol, at 22°C
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.64
membrane
microsome
Homo sapiens
16020
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.64
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.64
retinal pigment epithelium
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.64
all-trans-retinyl ester + H2O
-
704711
Homo sapiens
11-cis-retinol + a fatty acid
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
3.1.1.64
physiological function
RPE65 operates in a multiprotein complex with retinol dehydrogenase 5 and retinal G protein-coupled receptor in RPE microsomes
Homo sapiens
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.1.1.64
physiological function
RPE65 operates in a multiprotein complex with retinol dehydrogenase 5 and retinal G protein-coupled receptor in RPE microsomes
Homo sapiens