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Literature summary extracted from

  • Damerow, S.; Lamerz, A.C.; Haselhorst, T.; Fuehring, J.; Zarnovican, P.; von Itzstein, M.; Routier, F.H.
    Leishmania UDP-sugar pyrophosphorylase: the missing link in galactose salvage? (2010), J. Biol. Chem., 285, 878-887.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.64 expressed in Escherichia coli as a His-tagged fusion protein Leishmania major
2.7.7.64 into the vector pET22b for expression in Escherichia coli BL21DE3 cells Leishmania major

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.64 0.098
-
 UTP forward reaction, D-galactose 1-phosphate, pH 7.8, 25°C Leishmania major
2.7.7.64 0.098
-
 UTP forward reaction, co-substrate: D-galactose 1-phosphate, pH 7.8, 25°C Leishmania major
2.7.7.64 0.116
-
 UTP forward reaction, D-glucose 1-phosphate, pH 7.8, 25°C Leishmania major
2.7.7.64 0.116
-
 UTP forward reaction, co-substrate: D-glucose 1-phosphate, pH 7.8, 25°C Leishmania major
2.7.7.64 0.148
-
 UDP-galactose reverse reaction, pH 7.8, 25°C Leishmania major
2.7.7.64 0.148
-
 UDP-D-galactose reverse reaction, pH 7.8, 25°C Leishmania major
2.7.7.64 0.174
-
 UDP-glucose reverse reaction, pH 7.8, 25°C Leishmania major
2.7.7.64 0.174
-
 UDP-D-glucose reverse reaction, pH 7.8, 25°C Leishmania major
2.7.7.64 0.307
-
 diphosphate reverse reaction, UDP-galactose, pH 7.8, 25°C Leishmania major
2.7.7.64 0.307
-
 diphosphate reverse reaction, co-substrate: UDP-D-galactose, pH 7.8, 25°C Leishmania major
2.7.7.64 0.373
-
 UDP-beta-L-arabinopyranose reverse reaction, pH 7.8, 25°C Leishmania major
2.7.7.64 0.373
-
 UDP-L-arabinopyranose reverse reaction, pH 7.8, 25°C Leishmania major
2.7.7.64 0.383
-
 diphosphate reverse reaction, UDP-glucose, pH 7.8, 25°C Leishmania major
2.7.7.64 0.383
-
 diphosphate reverse reaction, co-substrate: UDP-D-glucose, pH 7.8, 25°C Leishmania major
2.7.7.64 0.728
-
 diphosphate reverse reaction, UDP-alpha-D-galacturonate, pH 7.8, 25°C Leishmania major
2.7.7.64 0.728
-
 diphosphate reverse reaction, co-substrate: UDP-D-galacturonate, pH 7.8, 25°C Leishmania major
2.7.7.64 0.79
-
 UDP-D-galacturonate reverse reaction, pH 7.8, 25°C Leishmania major
2.7.7.64 0.79
-
 UDP-alpha-D-galacturonate reverse reaction, pH 7.8, 25°C Leishmania major
2.7.7.64 0.86
-
 D-galactose 1-phosphate forward reaction, pH 7.8, 25°C Leishmania major
2.7.7.64 1.01
-
 diphosphate reverse reaction, co-substrate: UDP-L-arabinopyranose, pH 7.8, 25°C Leishmania major
2.7.7.64 1.018
-
 diphosphate reverse reaction, UDP-beta-L-arabinopyranose, pH 7.8, 25°C Leishmania major
2.7.7.64 1.7
-
 D-glucose 1-phosphate forward reaction, pH 7.8, 25°C Leishmania major
2.7.7.64 1.706
-
 D-glucose 1-phosphate forward reaction, pH 7.8, 25°C Leishmania major

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.64 66000
-
 USP-His6, determined by SDS-PAGE and Western blot analysis Leishmania major
2.7.7.64 69000
-
 predicted from cDNA Leishmania major
2.7.7.64 69700
-
 USP-His6, apparent molecular mass determind by gel filtration and standard kurve Leishmania major
2.7.7.64 70400
-
 theoretical Leishmania major

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.64 UTP + D-galactose 1-phosphate Leishmania major
-
 diphosphate + UDP-galactose
-
 r
2.7.7.64 UTP + D-glucose 1-phosphate Leishmania major
-
 diphosphate + UDP-glucose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.64 Leishmania major D3G6S4
-
-
2.7.7.64 Leishmania major Q4QE75
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.64 on a 1-ml HisTrap HP Ni2+-chelating, a HiPrep 26/10 desalting, and a 1-ml Q-Sepharose FF anion exchange column Leishmania major
2.7.7.64 using Ni-NTA chromatography, ion-exchange and gel filtration Leishmania major

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.64 additional information
-
 relative activity, alpha-D-galacturonate 1-phosphate, 1.6%, forward reaction, + UTP, pH 7.8, 25°C Leishmania major
2.7.7.64 additional information
-
 relative activity, D-galactose 1-phosphate, 100%, forward reaction, + UTP, pH 7.8, 25°C Leishmania major
2.7.7.64 additional information
-
 relative activity, D-glucose 1-phosphate, 88%, forward reaction, + UTP, pH 7.8, 25°C Leishmania major
2.7.7.64 additional information
-
 relative activity, D-xylopyranose 1-phosphate, 12%, forward reaction, + UTP, pH 7.8, 25°C Leishmania major
2.7.7.64 additional information
-
 relative activity, UDP-alpha-D-galacturonate, 7.1%, reverse reaction, + diphosphate, pH 7.8, 25°C Leishmania major
2.7.7.64 additional information
-
 relative activity, UDP-beta-L-arabinopyranose, 33%, reverse reaction, + diphosphate, pH 7.8, 25°C Leishmania major
2.7.7.64 additional information
-
 relative activity, UDP-galactose, 83%, reverse reaction, + diphosphate, pH 7.8, 25°C Leishmania major
2.7.7.64 additional information
-
 relative activity, UDP-glucose, 100%, reverse reaction, + diphosphate, pH 7.8, 25°C Leishmania major

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.64 D-galactose 1-phosphate + UTP
-
 Leishmania major UDP-D-galactose + diphosphate
-
 ?
2.7.7.64 D-galacturonate 1-phosphate + UTP
-
 Leishmania major UDP-D-galacturonate + diphosphate
-
 ?
2.7.7.64 D-glucose 1-phosphate + UTP
-
 Leishmania major UDP-D-glucose + diphosphate
-
 ?
2.7.7.64 L-arabinopyranose 1-phosphate + UTP
-
 Leishmania major UDP-L-arabinopyranose + diphosphate
-
 ?
2.7.7.64 UTP + alpha-D-galacturonate
-
 Leishmania major diphosphate + UDP-alpha-D-galacturonate
-
 r
2.7.7.64 UTP + beta-L-arabinopyranose
-
 Leishmania major diphosphate + UDP-beta-L-arabinopyranose
-
 r
2.7.7.64 UTP + D-galactose 1-phosphate
-
 Leishmania major diphosphate + UDP-galactose
-
 r
2.7.7.64 UTP + D-glucose 1-phosphate
-
 Leishmania major diphosphate + UDP-glucose
-
 r
2.7.7.64 UTP + D-xylopyranose 1-phosphate
-
 Leishmania major diphosphate + UDP-D-xylopyranose
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.64 monomer
-
 Leishmania major

Synonyms

EC Number Synonyms Comment Organism
2.7.7.64 UDP-sugar pyrophosphorylase
-
 Leishmania major
2.7.7.64 USP
-
 Leishmania major

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.64 25
-
 assay at Leishmania major
2.7.7.64 25
-
 activity assay Leishmania major

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.64 13
-
 UDP-D-galacturonate reverse reaction, pH 7.8, 25°C Leishmania major
2.7.7.64 13
-
 UDP-alpha-D-galacturonate reverse reaction, pH 7.8, 25°C Leishmania major
2.7.7.64 15
-
 diphosphate reverse reaction, UDP-alpha-D-galacturonate, pH 7.8, 25°C Leishmania major
2.7.7.64 15
-
 diphosphate reverse reaction, co-substrate: UDP-D-galacturonate, pH 7.8, 25°C Leishmania major
2.7.7.64 72
-
 UDP-beta-L-arabinopyranose reverse reaction, pH 7.8, 25°C Leishmania major
2.7.7.64 72
-
 UDP-L-arabinopyranose reverse reaction, pH 7.8, 25°C Leishmania major
2.7.7.64 81
-
 diphosphate reverse reaction, UDP-beta-L-arabinopyranose, pH 7.8, 25°C Leishmania major
2.7.7.64 81
-
 diphosphate reverse reaction, co-substrate: UDP-L-arabinopyranose, pH 7.8, 25°C Leishmania major
2.7.7.64 154
-
 UDP-galactose reverse reaction, pH 7.8, 25°C Leishmania major
2.7.7.64 154
-
 UDP-D-galactose reverse reaction, pH 7.8, 25°C Leishmania major
2.7.7.64 174
-
 diphosphate reverse reaction, UDP-galactose, pH 7.8, 25°C Leishmania major
2.7.7.64 174
-
 diphosphate reverse reaction, co-substrate: UDP-D-galactose, pH 7.8, 25°C Leishmania major
2.7.7.64 180
-
 UDP-glucose reverse reaction, pH 7.8, 25°C Leishmania major
2.7.7.64 180
-
 UDP-D-glucose reverse reaction, pH 7.8, 25°C Leishmania major
2.7.7.64 185
-
 diphosphate reverse reaction, UDP-glucose, pH 7.8, 25°C Leishmania major
2.7.7.64 185
-
 diphosphate reverse reaction, co-substrate: UDP-D-glucose, pH 7.8, 25°C Leishmania major
2.7.7.64 191
-
 D-glucose 1-phosphate forward reaction, pH 7.8, 25°C Leishmania major
2.7.7.64 201
-
 UTP forward reaction, D-galactose 1-phosphate, pH 7.8, 25°C Leishmania major
2.7.7.64 201
-
 UTP forward reaction, co-substrate: D-galactose 1-phosphate, pH 7.8, 25°C Leishmania major
2.7.7.64 209
-
 UTP forward reaction, D-glucose 1-phosphate, pH 7.8, 25°C Leishmania major
2.7.7.64 209
-
 UTP forward reaction, co-substrate: D-glucose 1-phosphate, pH 7.8, 25°C Leishmania major
2.7.7.64 219
-
 D-galactose 1-phosphate forward reaction, pH 7.8, 25°C Leishmania major

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.64 7.8
-
 assay at Leishmania major
2.7.7.64 7.8
-
 activity assay Leishmania major

General Information

EC Number General Information Comment Organism
2.7.7.64 physiological function USP is able to complement the growth defect of Escherichia coli galU mutant strain DEV6. This specific bacterial strain is unable to grow on agar-containing galactose as the only carbohydrate source due to a mutation in UDP-glucose pyrophosphorylase Leishmania major

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.7.64 16.7
-
 UDP-D-galacturonate reverse reaction, pH 7.8, 25°C Leishmania major
2.7.7.64 20.8
-
 diphosphate reverse reaction, co-substrate: UDP-D-galacturonate, pH 7.8, 25°C Leishmania major
2.7.7.64 81.2
-
 diphosphate reverse reaction, co-substrate: UDP-L-arabinopyranose, pH 7.8, 25°C Leishmania major
2.7.7.64 114
-
 D-glucose 1-phosphate forward reaction, pH 7.8, 25°C Leishmania major
2.7.7.64 198
-
 UDP-L-arabinopyranose reverse reaction, pH 7.8, 25°C Leishmania major
2.7.7.64 259
-
 D-galactose 1-phosphate forward reaction, pH 7.8, 25°C Leishmania major
2.7.7.64 492
-
 diphosphate reverse reaction, co-substrate: UDP-D-glucose, pH 7.8, 25°C Leishmania major
2.7.7.64 580
-
 diphosphate reverse reaction, co-substrate: UDP-D-galactose, pH 7.8, 25°C Leishmania major
2.7.7.64 1060
-
 UDP-D-glucose reverse reaction, pH 7.8, 25°C Leishmania major
2.7.7.64 1070
-
 UDP-D-galactose reverse reaction, pH 7.8, 25°C Leishmania major
2.7.7.64 1830
-
 UTP forward reaction, co-substrate: D-glucose 1-phosphate, pH 7.8, 25°C Leishmania major
2.7.7.64 2090
-
 UTP forward reaction, co-substrate: D-galactose 1-phosphate, pH 7.8, 25°C Leishmania major