EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.24 | recombinant TgPBGS is expressed in Escherichia coli as a His-tagged fusion protein | Toxoplasma gondii |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.1.24 | DELTA646-658 | a mutant enzyme lacking the C-terminal 13 amino acids distinguishing parasite PBGS from plant and animal enzymes is purified as a dimer, suggesting that the C-terminus is required for octamer stabilisation | Toxoplasma gondii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.24 | succinylacetone | - |
Toxoplasma gondii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.24 | 0.15 | - |
5-aminolevulinic acid | - |
Toxoplasma gondii |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.2.1.24 | apicoplast | - |
Toxoplasma gondii | 20011 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.24 | Mg2+ | enzyme responds to Mg2+ but not to Zn2+, enzyme shows two Mg2+ affinities | Toxoplasma gondii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.1.24 | 40000 | - |
recombinant TgPBGS also shows low levels of dimers, 2 * 40000 Da, SDS-PAGE | Toxoplasma gondii |
4.2.1.24 | 40000 | - |
recombinant TgPBGS is purified as a stable octamer, 8 * 40000 Da, SDS-PAGE | Toxoplasma gondii |
4.2.1.24 | 320000 | - |
gel filtration, recombinant TgPBGS is purified as a stable octamer | Toxoplasma gondii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.24 | Toxoplasma gondii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.24 | using Ni-NTA chromatography | Toxoplasma gondii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.1.24 | 0.4 | - |
- |
Toxoplasma gondii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.24 | 5-aminolevulinic acid + 5-aminolevulinic acid | - |
Toxoplasma gondii | porphobilinogen + 2 H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.1.24 | dimer | recombinant TgPBGS also shows low levels of dimers, 2 * 40000 Da, SDS-PAGE | Toxoplasma gondii |
4.2.1.24 | octamer | recombinant TgPBGS is purified as a stable octamer, 8 * 40000 Da, SDS-PAGE | Toxoplasma gondii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.24 | Porphobilinogen synthase | - |
Toxoplasma gondii |
4.2.1.24 | TgPBGS | - |
Toxoplasma gondii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.24 | 37 | - |
assay at | Toxoplasma gondii |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.24 | 7 | 8.5 | - |
Toxoplasma gondii |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
4.2.1.24 | 0.001 | - |
- |
Toxoplasma gondii | succinylacetone |