BRENDA - Enzyme Database show

RPE65, visual cycle retinol isomerase, is not inherently 11-cis-specific: support for a carbocation mechanism of retinol isomerization

Redmond, T.M.; Poliakov, E.; Kuo, S.; Chander, P.; Gentleman, S.; J. Biol. Chem. 285, 1919-1927 (2010)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.1.1.64
F61L
the mutant shows 1.02% activity compared to the wild type enzyme
Homo sapiens
3.1.1.64
F61W
the mutant shows 1.28% activity compared to the wild type enzyme
Homo sapiens
3.1.1.64
F61Y
the mutant shows 7.57% activity compared to the wild type enzyme
Homo sapiens
3.1.1.64
T147A
the mutant shows 12.63% activity compared to the wild type enzyme
Homo sapiens
3.1.1.64
T147C
the mutant shows 20.18% activity compared to the wild type enzyme
Homo sapiens
3.1.1.64
T147G
the mutant shows 30.02% activity compared to the wild type enzyme
Homo sapiens
3.1.1.64
T147S
the mutant shows 84% activity compared to the wild type enzyme
Homo sapiens
3.1.1.64
T147V
inactive
Homo sapiens
3.1.1.64
T147W
inactive
Homo sapiens
3.1.1.64
T147Y
inactive
Homo sapiens
3.1.1.64
W331F
the mutant shows 8.34% activity compared to the wild type enzyme
Homo sapiens
3.1.1.64
W331L
inactive
Homo sapiens
3.1.1.64
W331Q
inactive
Homo sapiens
3.1.1.64
W331Y
the mutant shows 26.72% activity compared to the wild type enzyme
Homo sapiens
3.1.1.64
Y239C
inactive
Homo sapiens
3.1.1.64
Y239D
inactive
Homo sapiens
3.1.1.64
Y239F
inactive
Homo sapiens
3.1.1.64
Y239L
inactive
Homo sapiens
3.1.1.64
Y239S
inactive
Homo sapiens
3.1.1.64
Y239T
inactive
Homo sapiens
3.1.1.64
Y239W
inactive
Homo sapiens
3.1.1.64
Y275F
the mutant shows 14.44% activity compared to the wild type enzyme
Homo sapiens
3.1.1.64
Y275I
inactive
Homo sapiens
3.1.1.64
Y275W
inactive
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.64
Homo sapiens
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.64
all-trans-retinyl ester + H2O
-
704672
Homo sapiens
11-cis-retinol + a fatty acid
-
-
-
?
3.1.1.64
additional information
RPE65 is not inherently 11-cis-specific and can produce both 11- and 13-cis isomers
704672
Homo sapiens
?
-
-
-
-
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.1.1.64
F61L
the mutant shows 1.02% activity compared to the wild type enzyme
Homo sapiens
3.1.1.64
F61W
the mutant shows 1.28% activity compared to the wild type enzyme
Homo sapiens
3.1.1.64
F61Y
the mutant shows 7.57% activity compared to the wild type enzyme
Homo sapiens
3.1.1.64
T147A
the mutant shows 12.63% activity compared to the wild type enzyme
Homo sapiens
3.1.1.64
T147C
the mutant shows 20.18% activity compared to the wild type enzyme
Homo sapiens
3.1.1.64
T147G
the mutant shows 30.02% activity compared to the wild type enzyme
Homo sapiens
3.1.1.64
T147S
the mutant shows 84% activity compared to the wild type enzyme
Homo sapiens
3.1.1.64
T147V
inactive
Homo sapiens
3.1.1.64
T147W
inactive
Homo sapiens
3.1.1.64
T147Y
inactive
Homo sapiens
3.1.1.64
W331F
the mutant shows 8.34% activity compared to the wild type enzyme
Homo sapiens
3.1.1.64
W331L
inactive
Homo sapiens
3.1.1.64
W331Q
inactive
Homo sapiens
3.1.1.64
W331Y
the mutant shows 26.72% activity compared to the wild type enzyme
Homo sapiens
3.1.1.64
Y239C
inactive
Homo sapiens
3.1.1.64
Y239D
inactive
Homo sapiens
3.1.1.64
Y239F
inactive
Homo sapiens
3.1.1.64
Y239L
inactive
Homo sapiens
3.1.1.64
Y239S
inactive
Homo sapiens
3.1.1.64
Y239T
inactive
Homo sapiens
3.1.1.64
Y239W
inactive
Homo sapiens
3.1.1.64
Y275F
the mutant shows 14.44% activity compared to the wild type enzyme
Homo sapiens
3.1.1.64
Y275I
inactive
Homo sapiens
3.1.1.64
Y275W
inactive
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.64
all-trans-retinyl ester + H2O
-
704672
Homo sapiens
11-cis-retinol + a fatty acid
-
-
-
?
3.1.1.64
additional information
RPE65 is not inherently 11-cis-specific and can produce both 11- and 13-cis isomers
704672
Homo sapiens
?
-
-
-
-