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Literature summary extracted from

  • Liavonchanka, A.; Rudolph, M.G.; Tittmann, K.; Hamberg, M.; Feussner, I.
    On the mechanism of a polyunsaturated fatty acid double bond isomerase from Propionibacterium acnes (2009), J. Biol. Chem., 284, 8005-8012.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.3.13 into the vector pGEX-6P-1 for expression in Escherichia coli cells Cutibacterium acnes

Protein Variants

EC Number Protein Variants Comment Organism
5.3.3.13 F168G/G169F inactive mutant Cutibacterium acnes
5.3.3.13 F168L inactive mutant Cutibacterium acnes
5.3.3.13 F193A mutant Cutibacterium acnes
5.3.3.13 R88A inactive mutant Cutibacterium acnes
5.3.3.13 R88S mutant Cutibacterium acnes
5.3.3.13 R88S/F193A mutant Cutibacterium acnes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.3.13 additional information
-
 additional information (11S-2H) linoleic acid, 0.00408, wild-type PAI Cutibacterium acnes
5.3.3.13 additional information
-
 additional information [11,11-2H2] linoleic acid, 0.00228, wild-type PAI Cutibacterium acnes
5.3.3.13 0.00482
-
 linoleic acid wild-type PAI Cutibacterium acnes
5.3.3.13 0.02577
-
 linoleic acid mutant R88S/F193A Cutibacterium acnes
5.3.3.13 0.03794
-
 linoleic acid mutant F193A Cutibacterium acnes
5.3.3.13 0.0657
-
 linoleic acid mutant R88S Cutibacterium acnes

Organism

EC Number Organism UniProt Comment Textmining
5.3.3.13 Cutibacterium acnes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.3.13
-
 Cutibacterium acnes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.3.13 linoleic acid
-
 Cutibacterium acnes ?
-
 ?
5.3.3.13 linoleic acid methyl ester
-
 Cutibacterium acnes (10E,12Z)-octadeca-10,12-dienoic acid methyl ester
-
 ?
5.3.3.13 linoleoyl-CoA
-
 Cutibacterium acnes (10Z,12Z)-octadeca-10,12-dienoyl-CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.3.13 PAI
-
 Cutibacterium acnes
5.3.3.13 polyunsaturated fatty acid double bond isomerase
-
 Cutibacterium acnes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.3.13 25
-
 kinetic assay Cutibacterium acnes

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.3.13 additional information
-
 additional information (11S-2H) linoleic acid, 3.51, wild-type PAI Cutibacterium acnes
5.3.3.13 additional information
-
 additional information [11,11-2H2] linoleic acid, 0.83, wild-type PAI Cutibacterium acnes
5.3.3.13 0.16
-
 linoleic acid mutant R88S/F193A Cutibacterium acnes
5.3.3.13 0.33
-
 linoleic acid mutant F193A Cutibacterium acnes
5.3.3.13 2.93
-
 linoleic acid mutant R88S Cutibacterium acnes
5.3.3.13 4.37
-
 linoleic acid wild-type PAI Cutibacterium acnes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.3.13 7.5
-
 activity and kinetic assay Cutibacterium acnes

Cofactor

EC Number Cofactor Comment Organism Structure
5.3.3.13 FAD
-
 Cutibacterium acnes