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Literature summary extracted from

  • Zhu, B.; Yao, P.; Tan, M.; Eriani, G.; Wang, E.
    tRNA-independent pretransfer editing by class I leucyl-tRNA synthetase (2009), J. Biol. Chem., 284, 3418-3424.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.4 expression in Escherichia coli Aquifex aeolicus

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.4 D373A mutant defective in post-transfer editing function Aquifex aeolicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.4 0.0028
-
 L-leucine 37°C Aquifex aeolicus
6.1.1.4 0.096
-
 L-norvaline 37°C Aquifex aeolicus
6.1.1.4 0.698
-
 L-isoleucine 37°C Aquifex aeolicus
6.1.1.4 0.983
-
 L-methionine 37°C Aquifex aeolicus

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.4 Aquifex aeolicus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.4 ATP + L-isoleucine + tRNALeu
-
 Aquifex aeolicus AMP + diphosphate + L-isoleucyl-tRNALeu
-
 ?
6.1.1.4 ATP + L-leucine + tRNALeu
-
 Aquifex aeolicus AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
6.1.1.4 ATP + L-methionine + tRNALeu
-
 Aquifex aeolicus AMP + diphosphate + L-methionyl-tRNALeu
-
 ?
6.1.1.4 ATP + L-norvaline + tRNALeu
-
 Aquifex aeolicus AMP + diphosphate + L-norvalyl-tRNALeu
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.4 1.9
-
 L-isoleucine 37°C Aquifex aeolicus
6.1.1.4 3.2
-
 L-methionine 37°C Aquifex aeolicus
6.1.1.4 12.6
-
 L-norvaline 37°C Aquifex aeolicus
6.1.1.4 26.3
-
 L-leucine 37°C Aquifex aeolicus

General Information

EC Number General Information Comment Organism
6.1.1.4 physiological function existence of a tRNA-independent pretransfer editing pathway in leucyltRNA synthetases from Aquifex aeolicus. This editing pathway is distinct from the post-transfer editing site and may occur at the synthetic catalytic site Aquifex aeolicus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.1.1.4 2.7
-
 L-isoleucine 37°C Aquifex aeolicus
6.1.1.4 3.3
-
 L-methionine 37°C Aquifex aeolicus
6.1.1.4 13
-
 L-norvaline 37°C Aquifex aeolicus
6.1.1.4 9400
-
 L-leucine 37°C Aquifex aeolicus