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Literature summary extracted from

  • Pena, M.M.; Melo, S.P.; Xing, Y.Y.; White, K.; Barbour, K.W.; Berger, F.G.
    The intrinsically disordered N-terminal domain of thymidylate synthase targets the enzyme to the ubiquitin-independent proteasomal degradation pathway (2009), J. Biol. Chem., 284, 31597-31607.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.45 expressed in RJK88.13 cells and in HEp2/500 cells Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.45 G5L/S6A the mutant has a relatively short half-life of about 1-2 h Mus musculus
2.1.1.45 P2A the mutant enzyme is quite stable relative to wild type enzyme with half-life of more than 36-48 h Mus musculus
2.1.1.45 P2D the mutant enzyme is quite stable relative to wild type enzyme with half-life of more than 36-48 h Mus musculus
2.1.1.45 P2G the mutant is unstable relative to wild type enzyme having half-life of 6-8 h similar to wild type Mus musculus
2.1.1.45 P2R the mutant enzyme is quite stable relative to wild type enzyme with half-life of more than 36-48 h Mus musculus
2.1.1.45 P2V the mutant is unstable relative to wild type enzyme having half-life of 1-2 h with half-life of more than 36-48 h Mus musculus
2.1.1.45 P2W the mutant enzyme is quite stable relative to wild type enzyme with half-life of more than 36-48 h Mus musculus
2.1.1.45 P2Y the mutant enzyme is quite stable relative to wild type enzyme with half-life of more than 36-48 h Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Mus musculus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.1.1.45 acetylation Nalpha-acetylation inhibits thymidylate degradation, Pro-2 protects the N terminus against Nalpha-acetylation Mus musculus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45 HiTrap Blue-Sepharose column chromatography and HiTrap Q column chromatography Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mus musculus dihydrofolate + dTMP
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.45 N5,N10-methylenetetrahydrofolate
-
 Mus musculus