EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.8.39 | Triton X-100 | 0.5%, stimulates | Methanothermobacter thermautotrophicus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.8.39 | expression in Escherichia coli | Methanothermobacter thermautotrophicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.B14 | phosphate | 820 mM phosphate buffer inhibits | Methanothermobacter thermautotrophicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.8.39 | membrane | - |
Pyrococcus furiosus | 16020 | - |
2.7.8.39 | membrane | - |
Methanothermobacter thermautotrophicus | 16020 | - |
3.1.3.B14 | membrane | - |
Methanothermobacter thermautotrophicus | 16020 | - |
5.5.1.4 | membrane | - |
Methanothermobacter thermautotrophicus | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.8.39 | Mg2+ | Mg2+ or Mn2+ required | Methanothermobacter thermautotrophicus | |
2.7.8.39 | Mn2+ | Mg2+ or Mn2+ required | Methanothermobacter thermautotrophicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.8.39 | 20764 | - |
x * 20764, calculated from sequence | Methanothermobacter thermautotrophicus |
2.7.8.39 | 20900 | - |
x * 20900, SDS-PAGE | Methanothermobacter thermautotrophicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.8.39 | CDP-2,3-bis-O-(phytanyl)-sn-glycerol + 1L-myo-inositol 1-phosphate | Methanothermobacter thermautotrophicus | the enzyme is involved in biosynthesis of archaetidyl-myo-inositol | CMP + 1-archaetidyl-1D-myo-inositol 3-phosphate | - |
? | |
3.1.3.B14 | 1-archaetidyl-1D-myo-inositol 3-phosphate + H2O | Methanothermobacter thermautotrophicus | the enzyme is involved in biosynthesis of archaetidyl-myo-inositol, a compound essential for glycolipid biosynthesis in archaea | 1-archaetidyl-1D-myo-inositol + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.8.39 | Methanothermobacter thermautotrophicus | O27726 | - |
- |
2.7.8.39 | Pyrococcus furiosus | - |
- |
- |
3.1.3.B14 | Methanothermobacter thermautotrophicus | - |
- |
- |
5.5.1.4 | Methanothermobacter thermautotrophicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.5.1.4 | partially purified by ammonium sulfate fractionation (50-70%) | Methanothermobacter thermautotrophicus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.8.39 | 0.02 | - |
pH 8.0, 60°C, crude enzyme | Methanothermobacter thermautotrophicus |
5.5.1.4 | 0.0003 | - |
crude enzyme, in 50mM Bicine buffer (pH 8.5), at 60°C | Methanothermobacter thermautotrophicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.8.39 | CDP-2,3-bis-O-(phytanyl)-sn-glycerol + 1L-myo-inositol 1-phosphate | - |
Methanothermobacter thermautotrophicus | CMP + 1-archaetidyl-1D-myo-inositol 3-phosphate | - |
? | |
2.7.8.39 | CDP-2,3-bis-O-(phytanyl)-sn-glycerol + 1L-myo-inositol 1-phosphate | the enzyme is involved in biosynthesis of archaetidyl-myo-inositol | Methanothermobacter thermautotrophicus | CMP + 1-archaetidyl-1D-myo-inositol 3-phosphate | - |
? | |
3.1.3.B14 | 1-archaetidyl-1D-myo-inositol 3-phosphate + H2O | - |
Methanothermobacter thermautotrophicus | 1-archaetidyl-1D-myo-inositol + phosphate | - |
? | |
3.1.3.B14 | 1-archaetidyl-1D-myo-inositol 3-phosphate + H2O | the enzyme is involved in biosynthesis of archaetidyl-myo-inositol, a compound essential for glycolipid biosynthesis in archaea | Methanothermobacter thermautotrophicus | 1-archaetidyl-1D-myo-inositol + phosphate | - |
? | |
5.5.1.4 | D-glucose 6-phosphate | - |
Methanothermobacter thermautotrophicus | 1L-myo-inositol 1-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.8.39 | ? | x * 20900, SDS-PAGE | Methanothermobacter thermautotrophicus |
2.7.8.39 | ? | x * 20764, calculated from sequence | Methanothermobacter thermautotrophicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.8.39 | AIP synthase | - |
Methanothermobacter thermautotrophicus |
2.7.8.39 | MTH1691 | - |
Methanothermobacter thermautotrophicus |
3.1.3.B14 | AIP phosphatase | - |
Methanothermobacter thermautotrophicus |
5.5.1.4 | Inositol 1-phosphate synthase | - |
Methanothermobacter thermautotrophicus |
5.5.1.4 | IP synthase | - |
Methanothermobacter thermautotrophicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.8.39 | 60 | - |
assay at | Methanothermobacter thermautotrophicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.8.39 | 8 | - |
assay at | Methanothermobacter thermautotrophicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
5.5.1.4 | NAD+ | - |
Methanothermobacter thermautotrophicus |