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Literature summary extracted from

  • Yokooji, Y.; Tomita, H.; Atomi, H.; Imanaka, T.
    Pantoate kinase and phosphopantothenate synthetase, two novel enzymes necessary for CoA biosynthesis in the archaea (2009), J. Biol. Chem., 284, 28137-28145.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.36 expression in Escherichia coli Thermococcus kodakarensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.169 CoA no inhibition in presence of 0.1 mM CoA, activity decrases by 20% in presence of 1 mM CoA Thermococcus kodakarensis
2.7.1.169 additional information no detectable decrase in activity at 0.1 mM: acetyl-CoA, dephospho-CoA, or 4'-phosphopantothenate Thermococcus kodakarensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.169 0.47
-
 ATP 42°C Thermococcus kodakarensis
2.7.1.169 1.2
-
 Pantoate 42°C Thermococcus kodakarensis
2.7.1.169 1.3
-
 pantothenate 42°C Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.169 32748
-
 2 * 32748, calculated from sequence Thermococcus kodakarensis
2.7.1.169 34000
-
 2 * 34000, SDS-PAGE Thermococcus kodakarensis
6.3.2.36 29843
-
 2 * 29843, calculated from sequence Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.169 ATP + pantoate Thermococcus kodakarensis
-
 ADP + 4-phosphopantoate
-
 ?
6.3.2.36 ATP + 4-phosphopantoate + beta-alanine Thermococcus kodakarensis the pantoate kinase/phosphopantothenate synthetase system represents the pathway for 4'-phosphopantothenate biosynthesis in Thermococcus kodakaraensis. The enzymes are necessary for CoA biosynthesis in this organism AMP + diphosphate + 4'-phosphopantothenate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.169 Thermococcus kodakarensis Q5JHF1
-
-
6.3.2.36 Thermococcus kodakarensis Q5JIZ8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.36 recombinant enzyme Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.169 ATP + pantoate
-
 Thermococcus kodakarensis ADP + 4-phosphopantoate
-
 ?
2.7.1.169 ATP + pantoate kcat/Km value with pantoate as a substrate is over 7fold higher than that observed with pantothenate Thermococcus kodakarensis ADP + 4-phosphopantoate
-
 ?
2.7.1.169 ATP + pantothenate kcat/Km value with pantoate as a substrate is over 7fold higher than that observed with pantothenate Thermococcus kodakarensis ADP + 4'-phosphopantothenate
-
 ?
6.3.2.36 ATP + 4-phosphopantoate + beta-alanine
-
 Thermococcus kodakarensis AMP + diphosphate + 4'-phosphopantothenate
-
 ?
6.3.2.36 ATP + 4-phosphopantoate + beta-alanine the pantoate kinase/phosphopantothenate synthetase system represents the pathway for 4'-phosphopantothenate biosynthesis in Thermococcus kodakaraensis. The enzymes are necessary for CoA biosynthesis in this organism Thermococcus kodakarensis AMP + diphosphate + 4'-phosphopantothenate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.169 dimer 2 * 34000, SDS-PAGE Thermococcus kodakarensis
2.7.1.169 dimer 2 * 32748, calculated from sequence Thermococcus kodakarensis
6.3.2.36 dimer 2 * 29843, calculated from sequence Thermococcus kodakarensis

Synonyms

EC Number Synonyms Comment Organism
2.7.1.169 PoK
-
 Thermococcus kodakarensis
2.7.1.169 TK2141 protein
-
 Thermococcus kodakarensis
6.3.2.36 phosphopantothenate synthetase
-
 Thermococcus kodakarensis
6.3.2.36 Pps
-
 Thermococcus kodakarensis
6.3.2.36 TK1686 protein
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.169 42
-
 assay Thermococcus kodakarensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.169 0.21
-
 pantothenate 42°C Thermococcus kodakarensis
2.7.1.169 1.48
-
 ATP 42°C Thermococcus kodakarensis
2.7.1.169 1.56
-
 Pantoate 42°C Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.36 8
-
 assay at Thermococcus kodakarensis

General Information

EC Number General Information Comment Organism
2.7.1.169 malfunction the deletion mutant strain grows well in media supplemented with 1 mM CoA. The deletion mutant strain could not grow at all in the absence of CoA Thermococcus kodakarensis
6.3.2.36 malfunction the deletion mutant strain grows well in media supplemented with 1 mM CoA. The deletion mutant strain can not grow at all in the absence of CoA Thermococcus kodakarensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.169 0.17
-
 pantothenate 42°C Thermococcus kodakarensis
2.7.1.169 1.3
-
 Pantoate 42°C Thermococcus kodakarensis
2.7.1.169 3.1
-
 ATP 42°C Thermococcus kodakarensis