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Literature summary extracted from

  • Mann, F.M.; Prisic, S.; Hu, H.; Xu, M.; Coates, R.M.; Peters, R.J.
    Characterization and inhibition of a class II diterpene cyclase from Mycobacterium tuberculosis: Implications for tuberculosis (2009), J. Biol. Chem., 284, 23574-23579.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.5.1.16 expressed in Escherichia coli strain C41 Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.5.1.16 (E,E,E)-geranylgeranyl diphosphate substrate inhibition Mycobacterium tuberculosis
5.5.1.16 15-aza-14,15-dihydrogeranylgeranyl diphosphate
-
Mycobacterium tuberculosis
5.5.1.16 15-aza-14,15-dihydrogeranylgeranyl thiolodiphosphate
-
Mycobacterium tuberculosis
5.5.1.16 Mg2+ the enzyme displays a rapid loss of activity as the Mg2+ concentration is raised above 0.1 mM Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.5.1.16 0.0016
-
(E,E,E)-geranylgeranyl diphosphate
-
Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.5.1.16 Mg2+ the enzyme requires Mg2+ and reacts most efficiently in the presence of low levels (0.1 mM) of Mg2+ Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.16 Mycobacterium tuberculosis
-
-
-
5.5.1.16 Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.5.1.16 amylose resin column chromatography Mycobacterium tuberculosis

Storage Stability

EC Number Storage Stability Organism
5.5.1.16 4°C, less than 24 h, the enzyme retains essentially full activity Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.16 (E,E,E)-geranylgeranyl diphosphate
-
Mycobacterium tuberculosis halimadienyl diphosphate
-
?
5.5.1.16 (E,E,E)-geranylgeranyl diphosphate
-
Mycobacterium tuberculosis H37Rv halimadienyl diphosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
5.5.1.16 HPS
-
Mycobacterium tuberculosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.5.1.16 -4
-
freezing leads to loss of about 10% activity over the course of 1 week Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.5.1.16 0.12
-
(E,E,E)-geranylgeranyl diphosphate
-
Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.5.1.16 0.018
-
(E,E,E)-geranylgeranyl diphosphate
-
Mycobacterium tuberculosis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
5.5.1.16 0.0000001
-
-
Mycobacterium tuberculosis 15-aza-14,15-dihydrogeranylgeranyl thiolodiphosphate
5.5.1.16 0.000002
-
-
Mycobacterium tuberculosis 15-aza-14,15-dihydrogeranylgeranyl diphosphate

General Information

EC Number General Information Comment Organism
5.5.1.16 metabolism class II diterpene cyclase that catalyzes the committed step in edaxadiene biosynthesis Mycobacterium tuberculosis