BRENDA - Enzyme Database show

Structural basis of the catalytic mechanism operating in open-closed conformers of lipocalin type prostaglandin D synthase

Kumasaka, T.; Aritake, K.; Ago, H.; Irikura, D.; Tsurumura, T.; Yamamoto, M.; Miyano, M.; Urade, Y.; Hayaishi, O.; J. Biol. Chem. 284, 22344-22352 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.3.99.2
for expression in Escherichia coli cells
Mus musculus
Crystallization (Commentary)
EC Number
Crystallization
Organism
5.3.99.2
mutant DELTA1-24_C65A L-PGDS is crystallized in two different crystal forms representing the conformational change between the open and closed states of the cavity of the beta-barrel, the structures are determined to resolutions of 2.1 and 2.0 A
Mus musculus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
5.3.99.2
DELTA1-24_C65A
mutant
Mus musculus
5.3.99.2
DELTA1-24_C65A+H111A
mutant
Mus musculus
5.3.99.2
DELTA1-24_C65A+P110A
mutant
Mus musculus
5.3.99.2
DELTA1-24_C65A+W54A
mutant
Mus musculus
5.3.99.2
DELTA1-24_L-PGDS
mutant
Mus musculus
5.3.99.2
DELTA1-24_L-PGDS+H111A
mutant
Mus musculus
5.3.99.2
DELTA1-24_L-PGDS+H116A
mutant
Mus musculus
5.3.99.2
DELTA1-24_L-PGDS+P110A
mutant
Mus musculus
5.3.99.2
DELTA1-24_L-PGDS+S45A
mutant
Mus musculus
5.3.99.2
DELTA1-24_L-PGDS+S45A/S81A
mutant
Mus musculus
5.3.99.2
DELTA1-24_L-PGDS+S45A/T67A
mutant
Mus musculus
5.3.99.2
DELTA1-24_L-PGDS+S45A/T67A/S81A
mutant
Mus musculus
5.3.99.2
DELTA1-24_L-PGDS+S81A
mutant
Mus musculus
5.3.99.2
DELTA1-24_L-PGDS+T67A
mutant
Mus musculus
5.3.99.2
DELTA1-24_L-PGDS+T67A/S81A
mutant
Mus musculus
5.3.99.2
DELTA1-24_L-PGDS+W45A
mutant
Mus musculus
5.3.99.2
DELTA1-24_L-PGDS+W54A/H111A
mutant
Mus musculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.99.2
U-46619
-
Mus musculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.2
0.0005
-
prostaglandin H2
mutant DELTA1-24_L-PGDS+S45A
Mus musculus
5.3.99.2
0.0008
-
prostaglandin H2
mutant DELTA1-24_L-PGDS; mutant DELTA1-24_L-PGDS+W54A/H111A
Mus musculus
5.3.99.2
0.0012
-
prostaglandin H2
mutant DELTA1-24_L-PGDS+S45A/S81A; mutant DELTA1-24_L-PGDS+S45A/T67A/S81A
Mus musculus
5.3.99.2
0.0013
-
prostaglandin H2
mutant DELTA1-24_L-PGDS+W45A
Mus musculus
5.3.99.2
0.0014
-
prostaglandin H2
mutant DELTA1-24_L-PGDS+H111A; mutant DELTA1-24_L-PGDS+H116A
Mus musculus
5.3.99.2
0.0015
-
prostaglandin H2
mutant DELTA1-24_L-PGDS+S81A
Mus musculus
5.3.99.2
0.0016
-
prostaglandin H2
mutant DELTA1-24_L-PGDS+P110A
Mus musculus
5.3.99.2
0.0021
-
prostaglandin H2
mutant DELTA1-24_L-PGDS+S45A/T67A; mutant DELTA1-24_L-PGDS+T67A/S81A
Mus musculus
5.3.99.2
0.0028
-
prostaglandin H2
mutant DELTA1-24_L-PGDS+T67A
Mus musculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.2
Prostaglandin H2
Mus musculus
-
Prostaglandin D2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.2
Mus musculus
O09114
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.99.2
-
Mus musculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.2
Prostaglandin H2
-
704523
Mus musculus
Prostaglandin D2
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
5.3.99.2
25
-
activity assay
Mus musculus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.3.99.2
8
-
activity assay
Mus musculus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.2
for expression in Escherichia coli cells
Mus musculus
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
5.3.99.2
mutant DELTA1-24_C65A L-PGDS is crystallized in two different crystal forms representing the conformational change between the open and closed states of the cavity of the beta-barrel, the structures are determined to resolutions of 2.1 and 2.0 A
Mus musculus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
5.3.99.2
DELTA1-24_C65A
mutant
Mus musculus
5.3.99.2
DELTA1-24_C65A+H111A
mutant
Mus musculus
5.3.99.2
DELTA1-24_C65A+P110A
mutant
Mus musculus
5.3.99.2
DELTA1-24_C65A+W54A
mutant
Mus musculus
5.3.99.2
DELTA1-24_L-PGDS
mutant
Mus musculus
5.3.99.2
DELTA1-24_L-PGDS+H111A
mutant
Mus musculus
5.3.99.2
DELTA1-24_L-PGDS+H116A
mutant
Mus musculus
5.3.99.2
DELTA1-24_L-PGDS+P110A
mutant
Mus musculus
5.3.99.2
DELTA1-24_L-PGDS+S45A
mutant
Mus musculus
5.3.99.2
DELTA1-24_L-PGDS+S45A/S81A
mutant
Mus musculus
5.3.99.2
DELTA1-24_L-PGDS+S45A/T67A
mutant
Mus musculus
5.3.99.2
DELTA1-24_L-PGDS+S45A/T67A/S81A
mutant
Mus musculus
5.3.99.2
DELTA1-24_L-PGDS+S81A
mutant
Mus musculus
5.3.99.2
DELTA1-24_L-PGDS+T67A
mutant
Mus musculus
5.3.99.2
DELTA1-24_L-PGDS+T67A/S81A
mutant
Mus musculus
5.3.99.2
DELTA1-24_L-PGDS+W45A
mutant
Mus musculus
5.3.99.2
DELTA1-24_L-PGDS+W54A/H111A
mutant
Mus musculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.99.2
U-46619
-
Mus musculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.2
0.0005
-
prostaglandin H2
mutant DELTA1-24_L-PGDS+S45A
Mus musculus
5.3.99.2
0.0008
-
prostaglandin H2
mutant DELTA1-24_L-PGDS; mutant DELTA1-24_L-PGDS+W54A/H111A
Mus musculus
5.3.99.2
0.0012
-
prostaglandin H2
mutant DELTA1-24_L-PGDS+S45A/S81A; mutant DELTA1-24_L-PGDS+S45A/T67A/S81A
Mus musculus
5.3.99.2
0.0013
-
prostaglandin H2
mutant DELTA1-24_L-PGDS+W45A
Mus musculus
5.3.99.2
0.0014
-
prostaglandin H2
mutant DELTA1-24_L-PGDS+H111A; mutant DELTA1-24_L-PGDS+H116A
Mus musculus
5.3.99.2
0.0015
-
prostaglandin H2
mutant DELTA1-24_L-PGDS+S81A
Mus musculus
5.3.99.2
0.0016
-
prostaglandin H2
mutant DELTA1-24_L-PGDS+P110A
Mus musculus
5.3.99.2
0.0021
-
prostaglandin H2
mutant DELTA1-24_L-PGDS+S45A/T67A; mutant DELTA1-24_L-PGDS+T67A/S81A
Mus musculus
5.3.99.2
0.0028
-
prostaglandin H2
mutant DELTA1-24_L-PGDS+T67A
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.2
Prostaglandin H2
Mus musculus
-
Prostaglandin D2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.2
-
Mus musculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.2
Prostaglandin H2
-
704523
Mus musculus
Prostaglandin D2
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
5.3.99.2
25
-
activity assay
Mus musculus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.3.99.2
8
-
activity assay
Mus musculus
General Information
EC Number
General Information
Commentary
Organism
5.3.99.2
physiological function
lipocalin type prostaglandin D synthase is a multi-functional protein acting as a somnogen (PGD2)-producing enzyme, an extracellular transporter of various lipophilic ligands, and an amyloid-beta chaperone in human cerebrospinal fluid
Mus musculus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
5.3.99.2
physiological function
lipocalin type prostaglandin D synthase is a multi-functional protein acting as a somnogen (PGD2)-producing enzyme, an extracellular transporter of various lipophilic ligands, and an amyloid-beta chaperone in human cerebrospinal fluid
Mus musculus