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Literature summary extracted from
- Structural basis of the catalytic mechanism operating in open-closed conformers of lipocalin type prostaglandin D synthase (2009), J. Biol. Chem., 284, 22344-22352.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.99.2 | for expression in Escherichia coli cells | Mus musculus |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 5.3.99.2 | mutant DELTA1-24_C65A L-PGDS is crystallized in two different crystal forms representing the conformational change between the open and closed states of the cavity of the beta-barrel, the structures are determined to resolutions of 2.1 and 2.0 A | Mus musculus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.3.99.2 | DELTA1-24_C65A | mutant | Mus musculus |
| 5.3.99.2 | DELTA1-24_C65A+H111A | mutant | Mus musculus |
| 5.3.99.2 | DELTA1-24_C65A+P110A | mutant | Mus musculus |
| 5.3.99.2 | DELTA1-24_C65A+W54A | mutant | Mus musculus |
| 5.3.99.2 | DELTA1-24_L-PGDS | mutant | Mus musculus |
| 5.3.99.2 | DELTA1-24_L-PGDS+H111A | mutant | Mus musculus |
| 5.3.99.2 | DELTA1-24_L-PGDS+H116A | mutant | Mus musculus |
| 5.3.99.2 | DELTA1-24_L-PGDS+P110A | mutant | Mus musculus |
| 5.3.99.2 | DELTA1-24_L-PGDS+S45A | mutant | Mus musculus |
| 5.3.99.2 | DELTA1-24_L-PGDS+S45A/S81A | mutant | Mus musculus |
| 5.3.99.2 | DELTA1-24_L-PGDS+S45A/T67A | mutant | Mus musculus |
| 5.3.99.2 | DELTA1-24_L-PGDS+S45A/T67A/S81A | mutant | Mus musculus |
| 5.3.99.2 | DELTA1-24_L-PGDS+S81A | mutant | Mus musculus |
| 5.3.99.2 | DELTA1-24_L-PGDS+T67A | mutant | Mus musculus |
| 5.3.99.2 | DELTA1-24_L-PGDS+T67A/S81A | mutant | Mus musculus |
| 5.3.99.2 | DELTA1-24_L-PGDS+W45A | mutant | Mus musculus |
| 5.3.99.2 | DELTA1-24_L-PGDS+W54A/H111A | mutant | Mus musculus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.99.2 | U-46619 | - |
Mus musculus |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.99.2 | 0.0005 | - |
prostaglandin H2 | mutant DELTA1-24_L-PGDS+S45A | Mus musculus | |
| 5.3.99.2 | 0.0008 | - |
prostaglandin H2 | mutant DELTA1-24_L-PGDS | Mus musculus | |
| 5.3.99.2 | 0.0008 | - |
prostaglandin H2 | mutant DELTA1-24_L-PGDS+W54A/H111A | Mus musculus | |
| 5.3.99.2 | 0.0012 | - |
prostaglandin H2 | mutant DELTA1-24_L-PGDS+S45A/S81A | Mus musculus | |
| 5.3.99.2 | 0.0012 | - |
prostaglandin H2 | mutant DELTA1-24_L-PGDS+S45A/T67A/S81A | Mus musculus | |
| 5.3.99.2 | 0.0013 | - |
prostaglandin H2 | mutant DELTA1-24_L-PGDS+W45A | Mus musculus | |
| 5.3.99.2 | 0.0014 | - |
prostaglandin H2 | mutant DELTA1-24_L-PGDS+H111A | Mus musculus | |
| 5.3.99.2 | 0.0014 | - |
prostaglandin H2 | mutant DELTA1-24_L-PGDS+H116A | Mus musculus | |
| 5.3.99.2 | 0.0015 | - |
prostaglandin H2 | mutant DELTA1-24_L-PGDS+S81A | Mus musculus | |
| 5.3.99.2 | 0.0016 | - |
prostaglandin H2 | mutant DELTA1-24_L-PGDS+P110A | Mus musculus | |
| 5.3.99.2 | 0.0021 | - |
prostaglandin H2 | mutant DELTA1-24_L-PGDS+S45A/T67A | Mus musculus | |
| 5.3.99.2 | 0.0021 | - |
prostaglandin H2 | mutant DELTA1-24_L-PGDS+T67A/S81A | Mus musculus | |
| 5.3.99.2 | 0.0028 | - |
prostaglandin H2 | mutant DELTA1-24_L-PGDS+T67A | Mus musculus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.99.2 | Prostaglandin H2 | Mus musculus | - |
Prostaglandin D2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.99.2 | Mus musculus | O09114 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.99.2 | - |
Mus musculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.99.2 | Prostaglandin H2 | - |
Mus musculus | Prostaglandin D2 | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.3.99.2 | L-PGDS | - |
Mus musculus |
| 5.3.99.2 | lipocalin type prostaglandin D synthase | - |
Mus musculus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.99.2 | 25 | - |
activity assay | Mus musculus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.99.2 | 8 | - |
activity assay | Mus musculus |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 5.3.99.2 | physiological function | lipocalin type prostaglandin D synthase is a multi-functional protein acting as a somnogen (PGD2)-producing enzyme, an extracellular transporter of various lipophilic ligands, and an amyloid-beta chaperone in human cerebrospinal fluid | Mus musculus |
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