EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.12.99.6 | HoxL | the auxiliary protein HoxL participates specifically in the maturation of the large subunit of the enzyme. It is essential for H2-oxidizing activity and enzyme-driven growth on H2. HoxL acts as a specific chaperone assisting the transfer of the Fe(CN-)2CO cofactor intermediate from the Hyp machinery to the enzyme | Cupriavidus necator H16 | |
1.12.99.6 | HoxV | the auxiliary protein HoxL participates specifically in the maturation of the large subunit of the enzyme. It is essential for H2-oxidizing activity and enzyme-driven growth on H2. HoxV functions as a scaffold delivering the Fe(CN-)2CO moiety to the precursor of the large subunit pre-HoxG of the enzyme | Cupriavidus necator H16 | |
1.12.99.6 | HypC | is essential for cofactor assembly | Cupriavidus necator H16 |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.12.99.6 | additional information | is oxygen-tolerant | Cupriavidus necator H16 |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.12.99.6 | membrane | - |
Cupriavidus necator H16 | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.12.99.6 | CN- | the auxiliary proteins HoxL and HoxV assist in assembly of the Fe(CN-)2CO moiety | Cupriavidus necator H16 | |
1.12.99.6 | CO | the auxiliary proteins HoxL and HoxV assist in assembly of the Fe(CN-)2CO moiety | Cupriavidus necator H16 | |
1.12.99.6 | Fe | the auxiliary proteins HoxL and HoxV assist in assembly of the Fe(CN-)2CO moiety | Cupriavidus necator H16 | |
1.12.99.6 | Ni2+ | Ni-Fe active site assembly, nickel is absent in samples of HoxV | Cupriavidus necator H16 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.12.99.6 | Cupriavidus necator H16 | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.12.99.6 | H2 + oxidized methylene blue | - |
Cupriavidus necator H16 | H+ + reduced methylene blue | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.12.99.6 | Mbh | - |
Cupriavidus necator H16 |
1.12.99.6 | [NiFe] hydrogenase | - |
Cupriavidus necator H16 |