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Literature summary extracted from

  • Ludwig, M.; Schubert, T.; Zebger, I.; Wisitruangsakul, N.; Saggu, M.; Strack, A.; Lenz, O.; Hildebrandt, P.; Friedrich, B.
    Concerted action of two novel auxiliary proteins in assembly of the active site in a membrane-bound [NiFe] hydrogenase (2009), J. Biol. Chem., 284, 2159-2168.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.12.99.6 HoxL the auxiliary protein HoxL participates specifically in the maturation of the large subunit of the enzyme. It is essential for H2-oxidizing activity and enzyme-driven growth on H2. HoxL acts as a specific chaperone assisting the transfer of the Fe(CN-)2CO cofactor intermediate from the Hyp machinery to the enzyme Cupriavidus necator H16
1.12.99.6 HoxV the auxiliary protein HoxL participates specifically in the maturation of the large subunit of the enzyme. It is essential for H2-oxidizing activity and enzyme-driven growth on H2. HoxV functions as a scaffold delivering the Fe(CN-)2CO moiety to the precursor of the large subunit pre-HoxG of the enzyme Cupriavidus necator H16
1.12.99.6 HypC is essential for cofactor assembly Cupriavidus necator H16

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.12.99.6 additional information is oxygen-tolerant Cupriavidus necator H16

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.99.6 membrane
-
Cupriavidus necator H16 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.99.6 CN- the auxiliary proteins HoxL and HoxV assist in assembly of the Fe(CN-)2CO moiety Cupriavidus necator H16
1.12.99.6 CO the auxiliary proteins HoxL and HoxV assist in assembly of the Fe(CN-)2CO moiety Cupriavidus necator H16
1.12.99.6 Fe the auxiliary proteins HoxL and HoxV assist in assembly of the Fe(CN-)2CO moiety Cupriavidus necator H16
1.12.99.6 Ni2+ Ni-Fe active site assembly, nickel is absent in samples of HoxV Cupriavidus necator H16

Organism

EC Number Organism UniProt Comment Textmining
1.12.99.6 Cupriavidus necator H16
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.99.6 H2 + oxidized methylene blue
-
Cupriavidus necator H16 H+ + reduced methylene blue
-
?

Synonyms

EC Number Synonyms Comment Organism
1.12.99.6 Mbh
-
Cupriavidus necator H16
1.12.99.6 [NiFe] hydrogenase
-
Cupriavidus necator H16