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Literature summary extracted from

  • Khromov, A.; Choudhury, N.; Stevenson, A.S.; Somlyo, A.V.; Eto, M.
    Phosphorylation-dependent autoinhibition of myosin light chain phosphatase accounts for Ca2+ sensitization force of smooth muscle contraction (2009), J. Biol. Chem., 284, 21569-21579.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.53 various parts of myosin phosphatase targeting subunit GST-fusions expressed in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.53 T696D part of myosin phosphatase targeting subunit, inhibitory site Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.53 fragment 654-880 of human myosin phosphatase targeting subunit GST-fusion
-
Sus scrofa
3.1.3.53 fragment 697-880 of human myosin phosphatase targeting subunit GST-fusion
-
Sus scrofa
3.1.3.53 additional information phosphorylation of myosin phosphatase targeting subunit on Thr696 and Thr853 (inhibitory sites) results in inhibition Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.53 Homo sapiens
-
-
-
3.1.3.53 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.53 GSH affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.53 aorta
-
Sus scrofa
-
3.1.3.53 HeLa cell
-
Homo sapiens
-
3.1.3.53 smooth muscle
-
Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.53 [myosin regulatory light chain] phosphate + H2O from chicken gizzard Sus scrofa [myosin regulatory light chain] + phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.53 myosin light chain phosphatase
-
Homo sapiens
3.1.3.53 myosin light chain phosphatase
-
Sus scrofa

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.3.53 0.0000011
-
-
Sus scrofa phosphorylated fragment 654-880 of human myosin phosphatase targeting subunit GST-fusion
3.1.3.53 0.00006
-
-
Sus scrofa phosphorylated fragment 697-880 of human myosin phosphatase targeting subunit GST-fusion
3.1.3.53 0.0014
-
-
Sus scrofa unphosphorylated fragment 654-880 of human myosin phosphatase targeting subunit GST-fusion
3.1.3.53 0.0061
-
-
Sus scrofa unphosphorylated fragment 697-880 of human myosin phosphatase targeting subunit GST-fusion