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Literature summary extracted from

  • Friedman, J.E.; Watson, J.A.; Lam, D.W.; Rokita, S.E.
    Iodotyrosine deiodinase is the first mammalian member of the NADH oxidase/flavin reductase superfamily (2006), J. Biol. Chem., 281, 2812-2819.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.21.1.1 expression in HEK-293 cell Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.21.1.1 0.00202
-
 3,5-diiodo-L-tyrosine cosubstrate NADPH, pH 7.4, 25°C Mus musculus
1.21.1.1 0.0044
-
 3,5-diiodo-L-tyrosine cosubstrate dithionite, pH 7.4, 25°C Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.21.1.1 membrane
-
 Sus scrofa 16020
-
1.21.1.1 membrane
-
 Mus musculus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.21.1.1 30000
-
 x * 30000, SDS-PAGE Sus scrofa
1.21.1.1 32800
-
 x * 32800, SDS-PAGE and calculated Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
1.21.1.1 Mus musculus Q9DCX8
-
-
1.21.1.1 Sus scrofa Q6TA49
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.21.1.1 solubilization by 0.1% CHAPS Sus scrofa
1.21.1.1 solubilization by 0.1% CHAPS Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.21.1.1 thyroid gland
-
 Sus scrofa
-
1.21.1.1 thyroid gland
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.21.1.1 3100
-
 pH 7.4, 25°C Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.1.1 3,5-diiodo-L-tyrosine + dithionite
-
 Mus musculus 3-iodo-L-tyrosine + ? + I-
-
 ?
1.21.1.1 3,5-diiodo-L-tyrosine + NADPH + H+
-
 Sus scrofa 3-iodo-L-tyrosine + NADP+ + I-
-
 ?
1.21.1.1 3,5-diiodo-L-tyrosine + NADPH + H+
-
 Mus musculus 3-iodo-L-tyrosine + NADP+ + I-
-
 ?

Subunits

EC Number Subunits Comment Organism
1.21.1.1 ? x * 30000, SDS-PAGE Sus scrofa
1.21.1.1 ? x * 32800, SDS-PAGE and calculated Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.21.1.1 0.11
-
 3,5-diiodo-L-tyrosine cosubstrate dithionite, pH 7.4, 25°C Mus musculus