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Literature summary extracted from

  • Gao, Y.; Wells, L.; Comer, F.I.; Parker, G.J.; Hart, G.W.
    Dynamic O-glycosylation of nuclear and cytosolic proteins: cloning and characterization of a neutral, cytosolic beta-N-acetylglucosaminidase from human brain (2001), J. Biol. Chem., 276, 9838-9845.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.52
-
 Bos taurus
3.2.1.169
-
 Chlorocebus aethiops

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.255 GlcNAc inhibited by GlcNAc, but not by GalNAc Bos taurus
3.2.1.52 N-acetyl-beta-D-glucosamine inhibited by GlcNAc, but not by GalNAc Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.255 cytosol mostly localized Bos taurus 5829
-
2.4.1.255 nucleus
-
 Bos taurus 5634
-
3.2.1.52 cytosol mostly localized Bos taurus 5829
-
3.2.1.52 nucleus
-
 Bos taurus 5634
-
3.2.1.169 cytoplasm
-
 Chlorocebus aethiops 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.255 103000
-
 SDS-PAGE Bos taurus
3.2.1.52 103000
-
 SDS-PAGE Bos taurus
3.2.1.169 135000
-
-
 Chlorocebus aethiops

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.255 Bos taurus
-
-
-
3.2.1.52 Bos taurus Q0V8R6
-
-
3.2.1.169 Chlorocebus aethiops
-
-
-
3.2.1.169 Homo sapiens P06865
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.255
-
 Bos taurus
3.2.1.52
-
 Bos taurus
3.2.1.169 nickel affinity chromatography Chlorocebus aethiops

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.255 brain
-
 Bos taurus
-
3.2.1.52 brain
-
 Bos taurus
-
3.2.1.169 brain expressed in every human tissue examined but is the highest in the brain, placenta, and pancreas Homo sapiens
-
3.2.1.169 COS-7 cell
-
 Chlorocebus aethiops
-
3.2.1.169 kidney lowest expression in lung, liver, and kidney Homo sapiens
-
3.2.1.169 liver lowest expression in lung, liver, and kidney Homo sapiens
-
3.2.1.169 lung lowest expression in lung, liver, and kidney Homo sapiens
-
3.2.1.169 pancreas expressed in every human tissue examined but is the highest in the brain, placenta, and pancreas Homo sapiens
-
3.2.1.169 placenta expressed in every human tissue examined but is the highest in the brain, placenta, and pancreas Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 1.84
-
 purified protein Bos taurus

Storage Stability

EC Number Storage Stability Organism
3.2.1.52 enzyme can be stored at -20°C Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.255 additional information p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc are no substrates. The cloned enzyme cleaves GlcNAc, but not GalNAc, from glycopeptides Bos taurus ?
-
 ?
3.2.1.52 4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
 Bos taurus 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.52 additional information p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc are no substrates. The cloned enzyme cleaves GlcNAc, but not GalNAc, from glycopeptides Bos taurus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.255 beta-N-acetylglucosaminidase
-
 Bos taurus
2.4.1.255 O-GlcNAc transferase
-
 Bos taurus
2.4.1.255 OGT
-
 Bos taurus
3.2.1.52 beta-N-acetylglucosaminidase
-
 Bos taurus
3.2.1.52 neutral hexosaminidase C
-
 Bos taurus
3.2.1.52 O-GlcNAcase
-
 Bos taurus
3.2.1.169 beta-N-acetylglucosaminidase
-
 Chlorocebus aethiops
3.2.1.169 beta-N-acetylglucosaminidase
-
 Homo sapiens
3.2.1.169 neutral hexosaminidase C
-
 Homo sapiens
3.2.1.169 O-GlcNAcase
-
 Chlorocebus aethiops
3.2.1.169 O-GlcNAcase
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.255 5.5 7
-
 Bos taurus
3.2.1.52 5.5 7
-
 Bos taurus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.255 Bos taurus
-
-
 4.63
3.2.1.52 Bos taurus
-
-
 4.63