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Literature summary extracted from

  • Oh, S.K.; Han, K.H.; Ryu, S.B.; Kang, H.
    Molecular cloning, expression, and functional analysis of a cis-prenyltransferase from Arabidopsis thaliana. Implications in rubber biosynthesis (2000), J. Biol. Chem., 275, 18482-18488.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.87 Triton X-100 not strictly dependent on TRiton X-100, maximum activity at 0.01% Triton X-100 Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.87 overexpression in Escherichia coli Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.87 Mg2+ no activity in absence of Mg2+. Rapid increase of enzyme activity with the addition of Mg2+ up 2 mM. Further addition of Mg2+ inhibits the activity Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.87 0.00013
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 30°C Arabidopsis thaliana
2.5.1.87 0.00362
-
 geranylgeranyl diphosphate pH 7.5, 30°C Arabidopsis thaliana
2.5.1.87 0.023
-
 isopentenyl diphosphate pH 7.5, 30°C Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.87 Mg2+ no activity in absence of Mg2+. Rapid increase of enzyme activity with the addition of Mg2+ up 2 mM. Further addition of Mg2+ inhibits the activity Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.87 33000
-
 x * 33000, calculated from sequence Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.87 (2E,6E)-farnesyl diphosphate + 21 isopentenyl diphosphate Arabidopsis thaliana trans,trans-farnesyl diphosphate is a better substrate than geranylgeranyl diphosphate. The enzyme catalyzes the formation of polyprenyl diphosphates with predominant carbon number C120. In vitro rubber biosynthesis analysis indicates that the Arabidopsis cis-prenyltransferase itself could not catalyze the formation of high molecular weight polyprenyl diphosphate such as natural rubber 21 diphosphate + di-trans,poly-cis tetracosaprenyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.87 Arabidopsis thaliana O80458
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.87
-
 Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.87 (2E,6E)-farnesyl diphosphate + 21 isopentenyl diphosphate trans,trans-farnesyl diphosphate is a better substrate than geranylgeranyl diphosphate. The enzyme catalyzes the formation of polyprenyl diphosphates with predominant carbon number C120. In vitro rubber biosynthesis analysis indicates that the Arabidopsis cis-prenyltransferase itself could not catalyze the formation of high molecular weight polyprenyl diphosphate such as natural rubber Arabidopsis thaliana 21 diphosphate + di-trans,poly-cis tetracosaprenyl diphosphate
-
 ?
2.5.1.87 (2E,6E)-farnesyl diphosphate + 21 isopentenyl diphosphate trans,trans-farnesyl diphosphate is a better substrate than geranylgeranyl diphosphate Arabidopsis thaliana 21 diphosphate + di-trans,poly-cis tetracosaprenyl diphosphate the enzyme synthesizes polyisoprenes with carbon number higher than 90. The peak activity is observed at the point that corresponds to the polyisoprene with carbon number C120 ?
2.5.1.87 geranylgeranyl diphosphate + 20 isopentenyl diphosphate trans,trans-farnesyl diphosphate is a better substrate than geranylgeranyl diphosphate Arabidopsis thaliana 20 diphosphate + di-trans,poly-cis-tetracosaprenyl diphosphate the enzyme synthesizes polyisoprenes with carbon number higher than 90. The peak activity is observed at the point that corresponds to the polyisoprene with carbon number C120 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.87 ? x * 33000, calculated from sequence Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.5.1.87 AcpT
-
 Arabidopsis thaliana
2.5.1.87 dehydrodolichyl diphosphate synthase 1
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.87 30
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.87 7.5
-
 assay at Arabidopsis thaliana

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.5.1.87 Arabidopsis thaliana calculated from sequence
-
 8.05