BRENDA - Enzyme Database show

Prostaglandin F2alpha synthase activities of aldo-keto reductase 1B1, 1B3 and 1B7

Kabututu, Z.; Manin, M.; Pointud, J.C.; Maruyama, T.; Nagata, N.; Lambert, S.; Lefrancois-Martinez, A.M.; Martinez, A.; Urade, Y.; J. Biochem. 145, 161-168 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.188
into vector pET-28a and expressed in Escherichia coli BL21 (DE3)
Mus musculus
1.1.1.188
into vector pET-28a and expressed in Escherichia coli BL21 (DE3); into vector pET-28a and expressed in Escherichia coli BL21 (DE3)
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.188
additional information
heat treatment of AKR1B1 at 100°C for 5 min completely inactivates the prostaglandin F2alpha synthase activity, which is strictly dependent on the presence of NADPH
Homo sapiens
1.1.1.188
additional information
heat treatment of AKR1B3 and AKR1B7 at 100°C for 5 min completely inactivates the prostaglandin F2alpha synthase activities, which is strictly dependent on the presence of NADPH
Mus musculus
1.1.1.188
sorbinil
;
Homo sapiens
1.1.1.188
sorbinil
significantly inhibits the prostaglandin F2alpha synthase activity of AKR1B3 in a non-competitive fashion, whereas AKR1B7 is not sensitive to the inhibitor
Mus musculus
1.1.1.188
Tolrestat
;
Homo sapiens
1.1.1.188
Tolrestat
significantly inhibits the prostaglandin F2alpha synthase activity of AKR1B3 in a non-competitive fashion, whereas AKR1B7 is not sensitive to the inhibitor
Mus musculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.188
0.0019
-
prostaglandin H2
-
Homo sapiens
1.1.1.188
0.0038
-
prostaglandin H2
AKR1B7
Mus musculus
1.1.1.188
0.0057
-
NADPH
-
Homo sapiens
1.1.1.188
0.0074
-
NADPH
AKR1B3
Mus musculus
1.1.1.188
0.0092
-
NADPH
AKR1B7
Mus musculus
1.1.1.188
0.0093
-
prostaglandin H2
AKR1B3
Mus musculus
1.1.1.188
0.018
-
prostaglandin H2
-
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.188
35000
-
x * 35000, SDS-PAGE; x * 35000, SDS-PAGE
Homo sapiens
1.1.1.188
35000
-
x * 35000, SDS-PAGE
Mus musculus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.188
Homo sapiens
P15121
-
-
1.1.1.188
Homo sapiens
P42330
-
-
1.1.1.188
Mus musculus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.188
to apparent homogeneity, on Ni-NTA resin and by gel filtration
Mus musculus
1.1.1.188
to apparent homogeneity, on Ni-NTA resin and by gel filtration; to apparent homogeneity, on Ni-NTA resin and by gel filtration
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.188
kidney
AKR1B3
Mus musculus
-
1.1.1.188
lung
-
Homo sapiens
-
1.1.1.188
placenta
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
prostaglandin H2 + NADPH
aldo-keto reductases AKR1B3 and AKR1B7 have prostaglandin F2alpha synthase activity
704349
Mus musculus
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
1.1.1.188
prostaglandin H2 + NADPH + H+
aldo-keto reductase has prostaglandin F2alpha synthase activity. AKR1B1 is a more efficient prostaglandin F2alpha synthase than AKR1C3
704349
Homo sapiens
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.188
?
x * 35000, SDS-PAGE; x * 35000, SDS-PAGE
Homo sapiens
1.1.1.188
?
x * 35000, SDS-PAGE
Mus musculus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.188
NADPH
;
Homo sapiens
1.1.1.188
NADPH
-
Mus musculus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.1.188
0.00026
-
Tolrestat
AKR1B3
Mus musculus
1.1.1.188
0.00089
-
sorbinil
AKR1B3
Mus musculus
1.1.1.188
0.0036
-
Tolrestat
-
Homo sapiens
1.1.1.188
0.0217
-
sorbinil
-
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.188
into vector pET-28a and expressed in Escherichia coli BL21 (DE3)
Homo sapiens
1.1.1.188
into vector pET-28a and expressed in Escherichia coli BL21 (DE3)
Mus musculus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.188
NADPH
-
Homo sapiens
1.1.1.188
NADPH
-
Mus musculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.188
additional information
heat treatment of AKR1B1 at 100°C for 5 min completely inactivates the prostaglandin F2alpha synthase activity, which is strictly dependent on the presence of NADPH
Homo sapiens
1.1.1.188
additional information
heat treatment of AKR1B3 and AKR1B7 at 100°C for 5 min completely inactivates the prostaglandin F2alpha synthase activities, which is strictly dependent on the presence of NADPH
Mus musculus
1.1.1.188
sorbinil
-
Homo sapiens
1.1.1.188
sorbinil
significantly inhibits the prostaglandin F2alpha synthase activity of AKR1B3 in a non-competitive fashion, whereas AKR1B7 is not sensitive to the inhibitor
Mus musculus
1.1.1.188
Tolrestat
-
Homo sapiens
1.1.1.188
Tolrestat
significantly inhibits the prostaglandin F2alpha synthase activity of AKR1B3 in a non-competitive fashion, whereas AKR1B7 is not sensitive to the inhibitor
Mus musculus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.1.188
0.00026
-
Tolrestat
AKR1B3
Mus musculus
1.1.1.188
0.00089
-
sorbinil
AKR1B3
Mus musculus
1.1.1.188
0.0036
-
Tolrestat
-
Homo sapiens
1.1.1.188
0.0217
-
sorbinil
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.188
0.0019
-
prostaglandin H2
-
Homo sapiens
1.1.1.188
0.0038
-
prostaglandin H2
AKR1B7
Mus musculus
1.1.1.188
0.0057
-
NADPH
-
Homo sapiens
1.1.1.188
0.0074
-
NADPH
AKR1B3
Mus musculus
1.1.1.188
0.0092
-
NADPH
AKR1B7
Mus musculus
1.1.1.188
0.0093
-
prostaglandin H2
AKR1B3
Mus musculus
1.1.1.188
0.018
-
prostaglandin H2
-
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.188
35000
-
x * 35000, SDS-PAGE
Homo sapiens
1.1.1.188
35000
-
x * 35000, SDS-PAGE
Mus musculus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.188
to apparent homogeneity, on Ni-NTA resin and by gel filtration
Homo sapiens
1.1.1.188
to apparent homogeneity, on Ni-NTA resin and by gel filtration
Mus musculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.188
kidney
AKR1B3
Mus musculus
-
1.1.1.188
lung
-
Homo sapiens
-
1.1.1.188
placenta
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.188
prostaglandin H2 + NADPH
aldo-keto reductases AKR1B3 and AKR1B7 have prostaglandin F2alpha synthase activity
704349
Mus musculus
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
1.1.1.188
prostaglandin H2 + NADPH + H+
aldo-keto reductase has prostaglandin F2alpha synthase activity. AKR1B1 is a more efficient prostaglandin F2alpha synthase than AKR1C3
704349
Homo sapiens
(5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.188
?
x * 35000, SDS-PAGE
Homo sapiens
1.1.1.188
?
x * 35000, SDS-PAGE
Mus musculus