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Literature summary extracted from

  • Morita, R.; Nakagawa, N.; Kuramitsu, S.; Masui, R.
    An O6-methylguanine-DNA methyltransferase-like protein from Thermus thermophilus interacts with a nucleotide excision repair protein (2008), J. Biochem., 144, 267-277.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.63 expressed in Escherichia coli Rosetta(DE3) cells Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.63 Thermus thermophilus
-
-
-
2.1.1.63 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.63 amylose resin column chromatography, Toyopearl-SuperQ column chromatography, ammonium sulfate precipitation, and Toyopearl-ether 650M column chromatography Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.63 additional information TTHA1564 can bind to DNA containing 6-O-methylguanine with higher affinity (9fold) than normal (unmethylated) DNA, but TTHA1564 alone does not possess methyltransferase activity Thermus thermophilus ?
-
 ?
2.1.1.63 additional information TTHA1564 can interact with nucleotide excision repair proteins and RNA polymerase Thermus thermophilus ?
-
 ?
2.1.1.63 additional information TTHA1564 can bind to DNA containing 6-O-methylguanine with higher affinity (9fold) than normal (unmethylated) DNA, but TTHA1564 alone does not possess methyltransferase activity Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
 ?
2.1.1.63 additional information TTHA1564 can interact with nucleotide excision repair proteins and RNA polymerase Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.63 O6-methylguanine-DNA methyltransferase
-
 Thermus thermophilus
2.1.1.63 OGT
-
 Thermus thermophilus
2.1.1.63 TTHA1564
-
 Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.63 additional information the enzyme requires no cofactor Thermus thermophilus