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Literature summary extracted from

  • Kasai, D.; Fujinami, T.; Abe, T.; Mase, K.; Katayama, Y.; Fukuda, M.; Masai, E.
    Uncovering the protocatechuate 2,3-cleavage pathway genes (2009), J. Bacteriol., 191, 6758-6768.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.85 expressed in Escherichia coli BL21(DE3) cells Paenibacillus sp.
1.14.13.33 praI gene expressed in Escherichia coli BL21(DE3) cells harboring pETI23 Paenibacillus sp.
5.3.2.6 gene praC, DNA and amino acid sequence determination and analysis, expression in Escherichia coli BL21(DE3) Paenibacillus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.85 53188
-
 x * 53188, calculated from amino acid sequence Paenibacillus sp.
1.2.1.85 58000
-
 x * 58000, SDS-PAGE Paenibacillus sp.
1.14.13.33 41000
-
 x * 41000, SDS-PAGE Paenibacillus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.2.6 2-hydroxymuconate Paenibacillus sp.
-
 4-oxalocrotonate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.85 Paenibacillus sp.
-
-
-
1.14.13.33 Paenibacillus sp.
-
-
-
5.3.2.6 Paenibacillus sp. C4TP07 formerly Bacillus macerans, gene praC
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.3.2.6 cell culture strain JJ-1b is able to grow on 4-hydroxybenzoate as a sole source of carbon and energy Paenibacillus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.33 0.36
-
 for 4-hydroxybenzoate in the presence of NADPH Paenibacillus sp.
1.14.13.33 0.47
-
 for 4-hydroxybenzoate in the presence of NADH Paenibacillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.85 2-hydroxymuconate-6-semialdehyde + NAD+ + H2O the activity of crude enzyme for 2-hydroxymuconate-6-semialdehyde in the presence of NAD+ is about 11 times higher than that achieved with NADP+ Paenibacillus sp. (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + H+
-
 ?
1.14.13.33 4-hydroxybenzoate + NAD(P)H + O2
-
 Paenibacillus sp. protocatechuate + NAD(P)+ + H2O
-
 ?
5.3.2.6 2-hydroxymuconate
-
 Paenibacillus sp. 4-oxalocrotonate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.85 ? x * 58000, SDS-PAGE Paenibacillus sp.
1.2.1.85 ? x * 53188, calculated from amino acid sequence Paenibacillus sp.
1.14.13.33 ? x * 41000, SDS-PAGE Paenibacillus sp.

Synonyms

EC Number Synonyms Comment Organism
1.2.1.85 HMS dehydrogenase
-
 Paenibacillus sp.
1.2.1.85 praB
-
 Paenibacillus sp.
1.14.13.33 4HB 3-hydroxylase
-
 Paenibacillus sp.
5.3.2.6 4-oxalocrotonate tautomerase
-
 Paenibacillus sp.
5.3.2.6 OCA
-
 Paenibacillus sp.
5.3.2.6 PRAC
-
 Paenibacillus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.2.6 35
-
 assay at Paenibacillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.2.6 7.3
-
 assay at Paenibacillus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.85 NAD+ highly specific for NAD+ Paenibacillus sp.
1.14.13.33 NADH
-
 Paenibacillus sp.
1.14.13.33 NADPH
-
 Paenibacillus sp.

General Information

EC Number General Information Comment Organism
5.3.2.6 metabolism the enzyme is involved in the degradation of 4-hydroxybenzoate via the protocatechuate 2,3-cleavage pathway, overview Paenibacillus sp.