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Literature summary extracted from

  • Gimmestad, M.; Ertesvag, H.; Heggeset, T.M.; Aarstad, O.; Svanem, B.I.; Valla, S.
    Characterization of three new Azotobacter vinelandii alginate lyases, one of which is involved in cyst germination (2009), J. Bacteriol., 191, 4845-4853.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.3 expressed in Escherichia coli DH5alpha cells Azotobacter vinelandii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.3 Ca2+ the activity of isoform AlyA3 is stimulated by Ca2+, an increase by 5fold is observed upon increasing the concentration of Ca2+ from 0.02 mM to 6.3 mM Azotobacter vinelandii
4.2.2.3 additional information the activities of isoforms AlyA1 and AlyA2 are not affected by addition of similar concentrations of Ca2+ Azotobacter vinelandii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.3 25900
-
isoform AlyA1, calculated from amino acid sequence Azotobacter vinelandii
4.2.2.3 26600
-
isoform AlyA2, calculated from amino acid sequence Azotobacter vinelandii
4.2.2.3 49400
-
isoform AlyA3, calculated from amino acid sequence Azotobacter vinelandii

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Azotobacter vinelandii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.3 HiTrap SP column chromatography (isoform AlyA1), HiTrap Q column chromatography (isoform AlyA2 and AlyA3), and butyl Sepharose column chromatography (isoform AlyA2) Azotobacter vinelandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 alginate isoforms AlyA1, AlyA2, and AlyA3 preferably cleave the bond between guluronic acid and mannuronic acid, resulting in a guluronic acid residue at the new reducing end, but isoform AlyA3 also degrades the other three possible bonds in alginate Azotobacter vinelandii unsaturated algino-oligosaccharides
-
?
4.2.2.3 G-alginate purified alpha-L-guluronic acid blocks containing more than 90% alpha-L-guluronic acid Azotobacter vinelandii ?
-
?
4.2.2.3 M-alginate pure 1-4-linked beta-D-mannuronic acid Azotobacter vinelandii ?
-
?
4.2.2.3 MG-alginate alginate epimerized by AlgE4 and containing 47% alpha-L-guluronic acid with no alpha-L-guluronic acid blocks, 100% activity Azotobacter vinelandii ? isoforms AlyA1, AlyA2, and AlyA3 produce mainly DELTAG and DELTAGMG as their end products, isoform AlyA3 produces as many trimers as it does dimers and tetramers ?

Synonyms

EC Number Synonyms Comment Organism
4.2.2.3 alginate lyase
-
Azotobacter vinelandii
4.2.2.3 AlyA1 isoform Azotobacter vinelandii
4.2.2.3 AlyA2 isoform Azotobacter vinelandii
4.2.2.3 AlyA3 isoform Azotobacter vinelandii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.3 6.8
-
isoform AlyA2 Azotobacter vinelandii
4.2.2.3 7.5
-
isoform AlyA3 Azotobacter vinelandii
4.2.2.3 7.8
-
isoform AlyA1 Azotobacter vinelandii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.2.3 Azotobacter vinelandii isoform AlyA3, calculated from amino acid sequence
-
4.2
4.2.2.3 Azotobacter vinelandii isoform AlyA2, calculated from amino acid sequence
-
5.6
4.2.2.3 Azotobacter vinelandii isoform AlyA1, calculated from amino acid sequence
-
9.7

General Information

EC Number General Information Comment Organism
4.2.2.3 malfunction strains lacking alyA3 germinate poorly compared to wild type cells Azotobacter vinelandii