EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.86 | dithiothreitol | stimulates activity | Mycobacterium tuberculosis | |
2.5.1.86 | Triton X-100 | detergent stimulates activity, maximally active in presence of 0.1% Triton X-100 | Mycobacterium tuberculosis | |
2.5.1.91 | dithiothreitol | - |
Mycobacterium tuberculosis | |
2.5.1.91 | additional information | stimulation by detergent | Mycobacterium tuberculosis | |
2.5.1.91 | Triton X-100 | maximum activity at 0.1% | Mycobacterium tuberculosis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.86 | expression in Escherichia coli | Mycobacterium tuberculosis |
2.5.1.91 | expression in Escherichia coli | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.86 | 0.029 | - |
neryl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.86 | 0.04 | - |
geranylgeranyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.86 | 0.084 | - |
(2E,6E)-farnesyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.86 | 0.089 | - |
isopentenyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.86 | 0.29 | - |
(2Z,6E)-farnesyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.86 | 0.49 | - |
geranyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.91 | 0.029 | - |
neryl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.91 | 0.04 | - |
(2E,6E,10E)-geranylgeranyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.91 | 0.084 | - |
(2E,6E)-farnesyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.91 | 0.089 | - |
isopentenyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.91 | 0.29 | - |
(2E,6Z)-farnesyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.91 | 0.49 | - |
geranyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.86 | Ca2+ | absolute requirement for divalent cations. Enzyme activity is optimal in the presence of 1 mM Mg2+. MnCl2 at 0.1 mM also supports the activity. CaCl2 and ZnCl2 are much less effective | Mycobacterium tuberculosis | |
2.5.1.86 | Mg2+ | absolute requirement for divalent cations. Enzyme activity is optimal in the presence of 1 mM Mg2+. MnCl2 at 0.1 mM also supports the activity. CaCl2 and ZnCl2 are much less effective | Mycobacterium tuberculosis | |
2.5.1.86 | Mn2+ | absolute requirement for divalent cations. Enzyme activity is optimal in the presence of 1 mM Mg2+. MnCl2 at 0.1 mM also supports the activity. CaCl2 and ZnCl2 are much less effective | Mycobacterium tuberculosis | |
2.5.1.86 | Zn2+ | absolute requirement for divalent cations. Enzyme activity is optimal in the presence of 1 mM Mg2+. MnCl2 at 0.1 mM also supports the activity. CaCl2 and ZnCl2 are much less effective | Mycobacterium tuberculosis | |
2.5.1.91 | Ca2+ | much less effective than Mg2+ | Mycobacterium tuberculosis | |
2.5.1.91 | Mg2+ | maximum activity at 1 mM | Mycobacterium tuberculosis | |
2.5.1.91 | Mn2+ | maximum activity at 0.1 mM, about 35% of the activity with Mg2+ | Mycobacterium tuberculosis | |
2.5.1.91 | additional information | absolute requirement for divalent kations | Mycobacterium tuberculosis | |
2.5.1.91 | Zn2+ | much less effective than Mg2+ | Mycobacterium tuberculosis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.86 | 36000 | - |
x * 36000, SDS-PAGE | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.86 | (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate | Mycobacterium tuberculosis | the catalytic efficiency is greatest when (2Z,6E)-farnesyl diphosphate is used as the allylic acceptor, suggesting that this is the natural substrate in vivo | 7 diphosphate + (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E)-decaprenyl diphosphate | - |
? | |
2.5.1.86 | (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate | Mycobacterium tuberculosis H37Rv | the catalytic efficiency is greatest when (2Z,6E)-farnesyl diphosphate is used as the allylic acceptor, suggesting that this is the natural substrate in vivo | 7 diphosphate + (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E)-decaprenyl diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.86 | Mycobacterium tuberculosis | P9WFF7 | - |
- |
2.5.1.86 | Mycobacterium tuberculosis H37Rv | P9WFF7 | - |
- |
2.5.1.91 | Mycobacterium tuberculosis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.86 | - |
Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.86 | (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate | - |
Mycobacterium tuberculosis | diphosphate + decaprenyl diphosphate + nonaprenyl diphosphate + octaprenyl diphosphate | the relative amounts of both decaprenyl diphosphate and octaprenyl diphosphate are greater than that of nonaprenyl diphosphate. Shorter chain length also produced. As the concentration of (2Z,6E)-farnesyl diphosphate is increased in the assays, there is an increase in the amount of the shorter products synthesized relative to decaprenyl diphosphate | ? | |
2.5.1.86 | (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate | - |
Mycobacterium tuberculosis H37Rv | diphosphate + decaprenyl diphosphate + nonaprenyl diphosphate + octaprenyl diphosphate | the relative amounts of both decaprenyl diphosphate and octaprenyl diphosphate are greater than that of nonaprenyl diphosphate. Shorter chain length also produced. As the concentration of (2Z,6E)-farnesyl diphosphate is increased in the assays, there is an increase in the amount of the shorter products synthesized relative to decaprenyl diphosphate | ? | |
2.5.1.86 | (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate | the catalytic efficiency is greatest when (2Z,6E)-farnesyl diphosphate is used as the allylic acceptor, suggesting that this is the natural substrate in vivo | Mycobacterium tuberculosis | 7 diphosphate + (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E)-decaprenyl diphosphate | - |
? | |
2.5.1.86 | (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate | (2Z,6E)-farnesyl diphosphate i.e. omega, E,Z-farnesyl diphosphate, the catalytic efficiency is greatest when (2Z,6E)-farnesyl diphosphate is used as the allylic acceptor | Mycobacterium tuberculosis | 7 diphosphate + (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E)-decaprenyl diphosphate | decaprenyl diphosphate is the predominant product synthesized, smaller amounts of intermediates of intermediates of shorter chain lengths also being produced. The amount of 40-carbon and 45-carbon products relative to 50-carbon products increases as the (2Z,6E)-farnesyl diphosphate concentration is increased, suggesting that the enzyme loses specificity as the substrate concentration increases | ? | |
2.5.1.86 | (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate | the catalytic efficiency is greatest when (2Z,6E)-farnesyl diphosphate is used as the allylic acceptor, suggesting that this is the natural substrate in vivo | Mycobacterium tuberculosis H37Rv | 7 diphosphate + (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E)-decaprenyl diphosphate | - |
? | |
2.5.1.86 | (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate | (2Z,6E)-farnesyl diphosphate i.e. omega, E,Z-farnesyl diphosphate, the catalytic efficiency is greatest when (2Z,6E)-farnesyl diphosphate is used as the allylic acceptor | Mycobacterium tuberculosis H37Rv | 7 diphosphate + (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E)-decaprenyl diphosphate | decaprenyl diphosphate is the predominant product synthesized, smaller amounts of intermediates of intermediates of shorter chain lengths also being produced. The amount of 40-carbon and 45-carbon products relative to 50-carbon products increases as the (2Z,6E)-farnesyl diphosphate concentration is increased, suggesting that the enzyme loses specificity as the substrate concentration increases | ? | |
2.5.1.86 | geranyl diphosphate + isopentenyl diphosphate | - |
Mycobacterium tuberculosis | diphosphate + decaprenyl diphosphate + nonaprenyl diphosphate + octaprenyl diphosphate | decaprenyl diphosphate, nonaprenyl diphosphate, and octaprenyl diphosphate are synthesized. The relative amounts of both decaprenyl diphosphate and octaprenyl diphosphate are greater than that of nonaprenyl diphosphate | ? | |
2.5.1.86 | geranyl diphosphate + isopentenyl diphosphate | - |
Mycobacterium tuberculosis H37Rv | diphosphate + decaprenyl diphosphate + nonaprenyl diphosphate + octaprenyl diphosphate | decaprenyl diphosphate, nonaprenyl diphosphate, and octaprenyl diphosphate are synthesized. The relative amounts of both decaprenyl diphosphate and octaprenyl diphosphate are greater than that of nonaprenyl diphosphate | ? | |
2.5.1.86 | geranylgeranyl diphosphate + isopentenyl diphosphate | - |
Mycobacterium tuberculosis | diphosphate + decaprenyl diphosphate + octaprenyl diphosphate + nonaprenyl diphosphate | the major product synthesized is octaprenyl diphosphate, followed by nonaprenyl diphosphate, decaprenyl diphosphate, and heptaprenyl diphosphate | ? | |
2.5.1.86 | additional information | the enzyme is not strictly specific for either the chain length or stereoconfiguration of the acceptor. No activity with dimethylallyl diphosphate | Mycobacterium tuberculosis | ? | - |
? | |
2.5.1.86 | additional information | the enzyme is not strictly specific for either the chain length or stereoconfiguration of the acceptor. No activity with dimethylallyl diphosphate | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
2.5.1.86 | neryl diphosphate + isopentenyl diphosphate | - |
Mycobacterium tuberculosis | diphosphate + decaprenyl diphosphate + nonaprenyl diphosphate + octaprenyl diphosphate | decaprenyl diphosphate, nonaprenyl diphosphate, and octaprenyl diphosphate are synthesized. The relative amounts of both decaprenyl diphosphate and octaprenyl diphosphate are greater than that of nonaprenyl diphosphate | ? | |
2.5.1.91 | (2E,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate | - |
Mycobacterium tuberculosis | 7 diphosphate + decaprenyl diphosphate | - |
? | |
2.5.1.91 | (2E,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate | - |
Mycobacterium tuberculosis | diphosphate + octaprenyl diphosphate | plus, in decreasing order, nonaprenyl diphosphate, decaprenyl diphosphate, and heptaprenyl diphosphate | ? | |
2.5.1.91 | (2E,6Z)-farnesyl diphosphate + isopentenyl diphosphate | highest catalytic efficiency | Mycobacterium tuberculosis | diphosphate + decaprenyl diphosphate | plus smaller amounts of intermediiates with shorter chain length | ? | |
2.5.1.91 | geranyl diphosphate + isopentenyl diphosphate | - |
Mycobacterium tuberculosis | diphosphate + decaprenyl diphosphate | plus some farnesyl diphosphate | ? | |
2.5.1.91 | neryl diphosphate + isopentenyl diphosphate | - |
Mycobacterium tuberculosis | diphosphate + nonaprenyl diphosphate | plus some farnesyl diphosphate | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.86 | ? | x * 36000, SDS-PAGE | Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.86 | Rv2361c | - |
Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.86 | 0.03 | - |
geranylgeranyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.86 | 0.06 | - |
geranyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.86 | 0.07 | - |
(2E,6E)-farnesyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.86 | 0.08 | - |
isopentenyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.86 | 0.16 | - |
neryl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.86 | 11.5 | - |
(2Z,6E)-farnesyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.91 | 0.03 | - |
(2E,6E,10E)-geranylgeranyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.91 | 0.052 | - |
neryl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.91 | 0.06 | - |
geranyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.91 | 0.072 | - |
(2E,6E)-farnesyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.91 | 0.08 | - |
isopentenyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.91 | 11.5 | - |
(2E,6Z)-farnesyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.86 | 7.5 | 8.5 | - |
Mycobacterium tuberculosis |
2.5.1.91 | 7.5 | 8.5 | - |
Mycobacterium tuberculosis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.86 | 0.122 | - |
geranyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.86 | 0.75 | - |
geranylgeranyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.86 | 0.899 | - |
isopentenyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.86 | 5.5 | - |
neryl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.86 | 39.66 | - |
(2Z,6E)-farnesyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.91 | 0.122 | - |
geranyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.91 | 0.75 | - |
(2E,6E,10E)-geranylgeranyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.91 | 0.86 | - |
(2E,6E)-farnesyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.91 | 0.89 | - |
isopentenyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.91 | 1.79 | - |
neryl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.91 | 39.65 | - |
(2E,6Z)-farnesyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis |