EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.1.4 | L-proline | Trypanosoma cruzi | the simplest mechanism for ProR isomerization of L-Pro to D-Pro entails the Cys130/Cys300 dyad in the thiolate/thiol forms, respectively, while His132 and Asp296 are in their neutral and carboxylate forms, in this scheme Cys130 is deprotonated either by a water molecule or an initially neutral form of the amine moiety of the substrate, thus, His132 and Asp296 do not serve a catalytic acid-base role in the racemization step but interact tightly with the ammonium moiety of the substrate, the ProR catalytic cycle involves 2 proton-transfer reactions in either direction of the racemization | D-proline | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.1.1.4 | Trypanosoma cruzi | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.1.4 | L-proline | the simplest mechanism for ProR isomerization of L-Pro to D-Pro entails the Cys130/Cys300 dyad in the thiolate/thiol forms, respectively, while His132 and Asp296 are in their neutral and carboxylate forms, in this scheme Cys130 is deprotonated either by a water molecule or an initially neutral form of the amine moiety of the substrate, thus, His132 and Asp296 do not serve a catalytic acid-base role in the racemization step but interact tightly with the ammonium moiety of the substrate, the ProR catalytic cycle involves 2 proton-transfer reactions in either direction of the racemization | Trypanosoma cruzi | D-proline | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.1.1.4 | homodimer | enzyme comprises of 2 alpha/beta units containing 414 amino acids which are separated by a deep cleft. Each monomer possesses an independent active site pocket, which is sequestered from water | Trypanosoma cruzi |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.1.1.4 | proline racemase | - |
Trypanosoma cruzi |
5.1.1.4 | ProR | - |
Trypanosoma cruzi |