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Literature summary extracted from

  • Dyer, J.H.; Maina, A.; Gomez, I.D.; Cadet, M.; Oeljeklaus, S.; Schiedel, A.C.
    Cloning, expression and purification of an acetoacetyl CoA thiolase from sunflower cotyledon (2009), Int. J. Biol. Sci., 5, 736-744.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.9 AACT, DNA and amino acid sequence determination and analysis, sequence comparison with Arabidopsis thaliana, expression of the active His-tagged enzyme in Escherichia coli TOP10 cells Helianthus annuus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.9 glyoxysome
-
 Helianthus annuus 9514
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.9 47000
-
 x * 47000, recombinant enzyme, SDS-PAGE Helianthus annuus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.9 Helianthus annuus D2IH11
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.9 recombinant His-tagged AACT 88fold to homogeneity by nickel affinity chromatography from Escherichia coli TOP10 cells Helianthus annuus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.9 cotyledon
-
 Helianthus annuus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.9 13.2
-
 purified native enzyme Helianthus annuus
2.3.1.9 15.78
-
 purified recombinant enzyme Helianthus annuus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.9 acetoacetyl-CoA + CoA coupled reaction assay in which the product of the thiolase reaction, acetyl-CoA, becomes the substrate for citrate synthase Helianthus annuus 2 acetyl-CoA
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.9 ? x * 47000, recombinant enzyme, SDS-PAGE Helianthus annuus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.9 AACT
-
 Helianthus annuus
2.3.1.9 acetoacetyl CoA thiolase
-
 Helianthus annuus
2.3.1.9 thiolase II
-
 Helianthus annuus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.9 8.5
-
 assay at Helianthus annuus