Literature summary extracted from

Kim, S.W.; Kim, D.U.; Kim, J.K.; Kang, L.W.; Cho, H.S.
Crystal structure of Pfu, the high fidelity DNA polymerase from Pyrococcus furiosus (2008), Int. J. Biol. Macromol., 42, 356-361.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.7.7.7	expressed in Escherichia coli BL21(DE3) cells	Pyrococcus furiosus

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
2.7.7.7	using 0.2 M ammonium sulfate, 0.1 M Na-cacodylate (pH 6.5), 5 mM dithiothreitol, 50 mM MnCl2, and 15% (w/v) PEG 8000	Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2.7.7.7	deoxynucleoside triphosphate + DNAn	Pyrococcus furiosus	-	diphosphate + DNAn+1	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.7.7	Pyrococcus furiosus	P61875	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.7.7.7	DEAE Sephacel gel filtration and HiTrap heparin column chromatography	Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.7.7.7	deoxynucleoside triphosphate + DNAn	-	Pyrococcus furiosus	diphosphate + DNAn+1	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
2.7.7.7	DNA polymerase	-	Pyrococcus furiosus
2.7.7.7	Pfu	a family B DNA polymerase	Pyrococcus furiosus

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Release 2023.1 (January 2023)