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Literature summary extracted from

  • Middleton, M.K.; Zukas, A.M.; Rubinstein, T.; Kinder, M.; Wilson, E.H.; Zhu, P.; Blair, I.A.; Hunter, C.A.; Pure, E.
    12/15-lipoxygenase-dependent myeloid production of interleukin-12 is essential for resistance to chronic toxoplasmosis (2009), Infect. Immun., 77, 5690-5700.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.31 PD146176 selective inhibitor of 12/15-LOX, macrophages treated with PD146176 elaborate reduced levels of interleukin-12 in response to Toxoplasma gondii antigen Mus musculus
1.13.11.33 PD146176 selective inhibitor of 12/15-LOX, macrophages treated with PD146176 elaborate reduced levels of interleukin-12 in response to Toxoplasma gondii antigen Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.31 Mus musculus
-
C57BL/6 mice
-
1.13.11.31 Mus musculus C57BL/6
-
C57BL/6 mice
-
1.13.11.33 Mus musculus
-
C57BL/6 mice
-
1.13.11.33 Mus musculus C57BL/6
-
C57BL/6 mice
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.31 brain 12/15-LOX is selectively enhanced in the periphery, 12/15-LOX-expressing myeloid cells are enriched in the brain during chronic toxoplasmosis Mus musculus
-
1.13.11.31 macrophage 12/15-LOX is a macrophage-selective regulator of interleukin-12 production Mus musculus
-
1.13.11.33 brain 12/15-LOX is selectively enhanced in the periphery, 12/15-LOX-expressing myeloid cells are enriched in the brain during chronic toxoplasmosis Mus musculus
-
1.13.11.33 macrophage 12/15-LOX is a macrophage-selective regulator of interleukin-12 production Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.31 arachidonate + O2
-
Mus musculus (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate
-
?
1.13.11.31 arachidonate + O2
-
Mus musculus C57BL/6 (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate
-
?
1.13.11.33 arachidonate + O2
-
Mus musculus (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate
-
?
1.13.11.33 arachidonate + O2
-
Mus musculus C57BL/6 (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate
-
?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.31 12/15-lipoxygenase
-
Mus musculus
1.13.11.31 12/15-LOX
-
Mus musculus
1.13.11.33 12/15-lipoxygenase
-
Mus musculus
1.13.11.33 12/15-LOX
-
Mus musculus

Expression

EC Number Organism Comment Expression
1.13.11.31 Mus musculus 12/15-LOX activity is enhanced during chronic, but not acute, toxoplasmosis up
1.13.11.33 Mus musculus 12/15-LOX activity is enhanced during chronic, but not acute, toxoplasmosis up

General Information

EC Number General Information Comment Organism
1.13.11.31 physiological function 12/15-LOX is a critical mediator of the chronic type 1 inflammatory response. Evolution of the immune response to Toxoplasma gondii is accompanied by an increasing requirement for 12/15-LOX mediated signaling. Although 12/15-LOX deficient mice are resistant to acute Toxoplasma gondii infection, 80% of 12/15-LOX-deficient mice die during chronic toxoplasmosis, compared to no deaths in wild-type controls. The enhanced susceptibility of 12/15-LOX-deficient mice to chronic toxoplasmosis is associated with reduced production of IL-12 and gamma interferon (IFN-gamma) that is not evident during acute infection. Ex vivo IFN-gamma production by 12/15-LOX-deficient splenocytes can be rescued by the addition of recombinant IL-12. 12/15-LOX does not play a role in macrophage killing of Toxoplasma gondii in vitro Mus musculus
1.13.11.33 physiological function 12/15-LOX is a critical mediator of the chronic type 1 inflammatory response. Evolution of the immune response to Toxoplasma gondii is accompanied by an increasing requirement for 12/15-LOX mediated signaling. Although 12/15-LOX deficient mice are resistant to acute Toxoplasma gondii infection, 80% of 12/15-LOX-deficient mice die during chronic toxoplasmosis, compared to no deaths in wild-type controls. The enhanced susceptibility of 12/15-LOX-deficient mice to chronic toxoplasmosis is associated with reduced production of IL-12 and gamma interferon (IFN-gamma) that is not evident during acute infection. Ex vivo IFN-gamma production by 12/15-LOX-deficient splenocytes can be rescued by the addition of recombinant IL-12. 12/15-LOX does not play a role in macrophage killing of Toxoplasma gondii in vitro Mus musculus