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Literature summary extracted from

  • Lin, C.T.; Tseng, W.C.; Hsiao, N.W.; Chang, H.H.; Ken, C.F.
    Characterization, molecular modelling and developmental expression of zebrafish manganese superoxide dismutase (2009), Fish Shellfish Immunol., 27, 318-324.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.1 DNA and amino acid sequence determination and analysis, real-time RT-PCR expression analysis, sequence comparisons, expression in Escherichia coli strain QC779 Danio rerio

General Stability

EC Number General Stability Organism
1.15.1.1 the enzyme is stable in presence of 4% SDS Danio rerio

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Mn a MnSOD Danio rerio

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.15.1.1 24989
-
 x * 24989, sequence calculation, MnSOD Danio rerio

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.15.1.1 2 O2.- + 2 H+ Danio rerio
-
 O2 + H2O2
-
 ?
1.15.1.1 2 O2.- + 2 H+ Danio rerio AB
-
 O2 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Danio rerio Q2TV65
-
-
1.15.1.1 Danio rerio AB Q2TV65
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 recombinant enzyme from Escherichia coli strain QC779 Danio rerio

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.15.1.1 additional information MnSOD developmental expression, overview Danio rerio
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 2 O2.- + 2 H+
-
 Danio rerio O2 + H2O2
-
 ?
1.15.1.1 2 O2.- + 2 H+
-
 Danio rerio AB O2 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 ? x * 24989, sequence calculation, MnSOD Danio rerio
1.15.1.1 More MnSOD molecular modelling of the highly conserved sequence, structure modelling and comparison with MnSODs from other organisms Danio rerio

Synonyms

EC Number Synonyms Comment Organism
1.15.1.1 manganese superoxide dismutase
-
 Danio rerio
1.15.1.1 Mn-SOD
-
 Danio rerio

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.15.1.1 additional information
-
 thermal inactivation kinetics fit the first-order inactivation rate equation, recombinant enzyme Danio rerio
1.15.1.1 70
-
 purified recombinant enzyme, half-life is 48 min Danio rerio
1.15.1.1 80
-
 purified recombinant enzyme, 48% remaining activity after 10 min Danio rerio
1.15.1.1 90
-
 purified recombinant enzyme, inactivation after 10 min Danio rerio

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.15.1.1 2.2 11.2 high enzyme activity Danio rerio

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.15.1.1 Danio rerio sequence calculation, MnSOD
-
 8.29