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Literature summary extracted from

  • Payne, K.A.; Hough, D.W.; Danson, M.J.
    Discovery of a putative acetoin dehydrogenase complex in the hyperthermophilic archaeon Sulfolobus solfataricus (2010), FEBS Lett., 584, 1231-1234.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.190 component E1alpha Saccharolobus solfataricus
2.3.1.190 component E1beta Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.190 0.0028
-
 acetoin E1 complex, 80°C, pH 7.0 Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.190 142000
-
 gel filtration, E1 complex Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.190 Saccharolobus solfataricus Q97YF5 E1beta subunit
-
2.3.1.190 Saccharolobus solfataricus Q97YF6 E1alpha subunit
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.190 acetoin + 2,6-dichlorophenolindophenol E1 complex Saccharolobus solfataricus acetaldehyde + ?
-
 ?
2.3.1.190 diacetyl + 2,6-dichlorophenolindophenol E1 complex, 59% of the activity with acetoin Saccharolobus solfataricus ?
-
 ?
2.3.1.190 methyl acetoin + 2,6-dichlorophenolindophenol E1 complex, 107% of the activity with acetoin Saccharolobus solfataricus ? + ?
-
 ?
2.3.1.190 additional information no detectable activity with 2-oxoglutarate, pyruvate or the branched-chain 2-oxoacids, 4-methyl-2-oxopentanoate, 3-methyl-2-oxopentanoate and 3-methyl-2-oxobutanoate Saccharolobus solfataricus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.190 More component E1, i.e. acetoin:2,6-dichlorophenolindophenol oxidoreductase, exhibits tetrameric alpha2beta2 structure, with alpha, 43000 and beta, 33000 Da, SDS-PAGE Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.190 80 85
-
 Saccharolobus solfataricus