BRENDA - Enzyme Database

Identification of two active functional domains of human adenylate kinase 5

Solaroli, N.; Panayiotou, C.; Johansson, M.; Karlsson, A.; FEBS Lett. 583, 2872-2876 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.4.3
expressed in Escherichia coli Rosetta (DE3) cells
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.3
0.172
-
AMP
adenylate kinase 5 domain AK5p1, with ATP as phosphate donor
Homo sapiens
2.7.4.3
2
-
dAMP
Km above 2.0 mM, adenylate kinase 5 domain AK5p1, with ATP as phosphate donor
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.4.3
cytosol
-
Homo sapiens
5829
-
2.7.4.3
nucleus
-
Homo sapiens
5634
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.4.3
Homo sapiens
Q9Y6K8
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.4.3
TALON metal affinity resin column chromatography
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.3
ATP + AMP
adenylate kinase 5 domain AK5p1, at the assay conditions used, and at lower concentrations of substrate, AK5p1 shows generally a higher affinity for AMP compared to dAMP
703721
Homo sapiens
ADP + ADP
-
-
-
?
2.7.4.3
ATP + CMP
adenylate kinase 5 domain AK5p1, the relative efficiency of CMP is about 15% compared to AMP
703721
Homo sapiens
ADP + CDP
-
-
-
?
2.7.4.3
ATP + dAMP
adenylate kinase 5 domain AK5p1
703721
Homo sapiens
ADP + dADP
-
-
-
?
2.7.4.3
ATP + dCMP
adenylate kinase 5 domain AK5p1, the relative efficiency of dCMP is about 15% compared to AMP
703721
Homo sapiens
ADP + dCDP
-
-
-
?
2.7.4.3
additional information
AK5p1 phosphorylates AMP, CMP, dAMP and dCMP with ATP or GTP as phosphate donors, AK5p2 phosphorylates AMP, CMP and dAMP when ATP is used as phosphate donor and AMP, CMP and dCMP with GTP as phosphate donor, AK5p2 cannot phosphorylate dAMP in the presence of GTP
703721
Homo sapiens
?
-
-
-
-
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.3
expressed in Escherichia coli Rosetta (DE3) cells
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.3
0.172
-
AMP
adenylate kinase 5 domain AK5p1, with ATP as phosphate donor
Homo sapiens
2.7.4.3
2
-
dAMP
Km above 2.0 mM, adenylate kinase 5 domain AK5p1, with ATP as phosphate donor
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.4.3
cytosol
-
Homo sapiens
5829
-
2.7.4.3
nucleus
-
Homo sapiens
5634
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.3
TALON metal affinity resin column chromatography
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.3
ATP + AMP
adenylate kinase 5 domain AK5p1, at the assay conditions used, and at lower concentrations of substrate, AK5p1 shows generally a higher affinity for AMP compared to dAMP
703721
Homo sapiens
ADP + ADP
-
-
-
?
2.7.4.3
ATP + CMP
adenylate kinase 5 domain AK5p1, the relative efficiency of CMP is about 15% compared to AMP
703721
Homo sapiens
ADP + CDP
-
-
-
?
2.7.4.3
ATP + dAMP
adenylate kinase 5 domain AK5p1
703721
Homo sapiens
ADP + dADP
-
-
-
?
2.7.4.3
ATP + dCMP
adenylate kinase 5 domain AK5p1, the relative efficiency of dCMP is about 15% compared to AMP
703721
Homo sapiens
ADP + dCDP
-
-
-
?
2.7.4.3
additional information
AK5p1 phosphorylates AMP, CMP, dAMP and dCMP with ATP or GTP as phosphate donors, AK5p2 phosphorylates AMP, CMP and dAMP when ATP is used as phosphate donor and AMP, CMP and dCMP with GTP as phosphate donor, AK5p2 cannot phosphorylate dAMP in the presence of GTP
703721
Homo sapiens
?
-
-
-
-