Literature summary extracted from
Posner, M.G.; Upadhyay, A.; Bagby, S.; Hough, D.W.; Danson, M.J.
A unique lipoylation system in the Archaea. Lipoylation in Thermoplasma acidophilum requires two proteins (2009), FEBS J., 276, 4012-4022.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
6.3.1.20 |
ATP |
assay in the presence of |
Thermoplasma acidophilum |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
6.3.1.20 |
expressed in Escherichia coli as a His-tagged fusion protein |
Thermoplasma acidophilum |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
6.3.1.20 |
additional information |
LplA and CTD encoding genes are expressed as fusion proteins in Escherichia coli by omitting the stop codon of lplA and the start codon of ctd. Fusion protein is shown to be catalytically active |
Thermoplasma acidophilum |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
6.3.1.20 |
MgCl2 |
assay in the presence of |
Thermoplasma acidophilum |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
6.3.1.20 |
11000 |
- |
SDS-PAGE, molecular weight of HisCTD fusion protein |
Thermoplasma acidophilum |
6.3.1.20 |
32000 |
- |
SDS-PAGE, molecular weight of HisLplA fusion protein |
Thermoplasma acidophilum |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
6.3.1.20 |
additional information |
Thermoplasma acidophilum |
it is shown that E2 lipoyl domain peptide, LplA and CTD are physically associated. LplA and CTD exist in a ratio 1:1 |
? |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
6.3.1.20 |
Thermoplasma acidophilum |
Q9HKT1 |
lipoylation occurs in vivo in Thermoplasma acidophilum but requires two proteins LplA and CTD |
- |
6.3.1.20 |
Thermoplasma acidophilum |
Q9HKT2 |
lipoylation occurs in vivo in Thermoplasma acidophilum but requires two proteins LplA and CTD |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
6.3.1.20 |
using His-tag affinity purification |
Thermoplasma acidophilum |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
6.3.1.20 |
additional information |
it is shown that E2 lipoyl domain peptide, LplA and CTD are physically associated. LplA and CTD exist in a ratio 1:1 |
Thermoplasma acidophilum |
? |
- |
? |
|
6.3.1.20 |
additional information |
when the Thermoplasma acidophilum E2 lipoyl domain peptide is incubated with either HisLplA or HisCTD in the presence of ATP, MgCl2 and lipoic acid, no lipoylation is detected. Incubation with both HisLplA and HisCTD in the one reaction results in the lipoylation of the E2 lipoyl domain peptide as indicated by nondenaturing PAGE |
Thermoplasma acidophilum |
? |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
6.3.1.20 |
CTD |
- |
Thermoplasma acidophilum |
6.3.1.20 |
LPLA |
- |
Thermoplasma acidophilum |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
6.3.1.20 |
25 |
- |
assay at |
Thermoplasma acidophilum |