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Literature summary extracted from

  • Yoshida, A.; Tomita, T.; Kono, H.; Fushinobu, S.; Kuzuyama, T.; Nishiyama, M.
    Crystal structures of the regulatory subunit of Thr-sensitive aspartate kinase from Thermus thermophilus (2009), FEBS J., 276, 3124-3136.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.4
-
 Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.2.4 enzyme free or in complex with L-threonine, hanging drop vapor diffusion method, using 0.1 M sodium acetate (pH 5.0) and 1.2-2.0 M NaCl Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.4 L-threonine
-
 Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.4 17700
-
 aspartate kinase beta, calculated from amino acid sequence Thermus thermophilus
2.7.2.4 21700
-
 aspartate kinase beta, gel filtration Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.4 ATP + L-aspartate Thermus thermophilus
-
 ADP + 4-phospho-L-aspartate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.4 Thermus thermophilus P61489
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.4
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.4 ATP + L-aspartate
-
 Thermus thermophilus ADP + 4-phospho-L-aspartate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.2.4 homodimer aspartate kinase beta in complex with L-threonine, X-ray crystallography Thermus thermophilus
2.7.2.4 monomer or dimer aspartate kinase beta is present in equilibrium between a monomer and dimer, ultracentrifugation Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
2.7.2.4 AKbeta
-
 Thermus thermophilus
2.7.2.4 Thr-sensitive aspartate kinase
-
 Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.2.4 91.7
-
 the melting temperature in the absence of any additive is at 91.7°C Thermus thermophilus