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Literature summary extracted from

  • Wolthers, K.R.; Scrutton, N.S.
    Cobalamin uptake and reactivation occurs through specific protein interactions in the methionine synthase-methionine synthase reductase complex (2009), FEBS J., 276, 1942-1951.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.13 aquacob(III)alamin the addition of 0.05 mM aquacob(III)alamin to the crude extract results in an about 13fold increase in activity which is not greatly affected by the addition of cytochrome P450 reductase or reductase domain of neuronal nitric oxide synthase Homo sapiens
2.1.1.13 methionine synthase reductase restores the activity of human methionine synthase through reductive methylation of methionine synthase-bound cob(II)alamin. Oxidized methionine synthase reductase does not reactivate methionine synthase, the presence of methionine synthase reductase along with AqCbl or MeCbl causes a dramatic increase in methionine synthase activity (7fold for AqCbl and 20fold for MeCbl) as compared to the cofactor alone Homo sapiens
2.1.1.13 methylcob(III)alamin the addition of 0.05 mM methylcob(III)alamin to the crude extract results in an about 6fold increase in activity which is not greatly affected by the addition of cytochrome P450 reductase or reductase domain of neuronal nitric oxide synthase Homo sapiens
2.1.1.13 NADPH reactivation of methionine synthase is dependent on NADPH concentration in a hyperbolic manner with maximum activity at about 0.2 mM Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.13 expressed in Pichia pastoris cells Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.13 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.13 Q-Sepharose column chromatography and cobalamin affinity chromatography Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.13 0.0005
-
 recombinant enzyme from crude extract, in 0.2 M potassium phosphate buffer (pH 7.2), at 37°C Homo sapiens
2.1.1.13 1.54
-
 recombinant enzyme after 3669fold purification, in 0.2 M potassium phosphate buffer (pH 7.2), at 37°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.13 N5-methyltetrahydropteroylmonoglutamate + L-homocysteine
-
 Homo sapiens tetrahydropteroylmonoglutamate + L-methionine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.13 MetS
-
 Homo sapiens
2.1.1.13 N-methyltetrahydrofolate:L-homocysteine methyltransferase
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.13 Cobalamin cobalamin serves as an intermediary in methyl transfer reactions, and it cycles between the methylcob(III)alamin and cob(I)alamin forms Homo sapiens