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Literature summary extracted from
- Absence of a catalytic water confers resistance to the neurotoxin gabaculine (2010), FASEB J., 24, 404-414.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.3.8 | expressed in Escherichia coli | Synechococcus sp. |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 5.4.3.8 | wild type enzyme and mutant enzyme M248I are crystallized by the vapor diffusion method, using 11.5% (w/v) PEG 8.000 and 150 mM magnesium acetate, pH 6.8 | Synechococcus sp. |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.4.3.8 | M248I | the M248I point mutation confers about 100fold increased gabaculine resistance to GSAM | Synechococcus sp. |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.3.8 | gabaculine | potent inhibitor | Synechococcus sp. |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.3.8 | Synechococcus sp. | P24630 | strain PCC 6301 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.3.8 | - |
Synechococcus sp. |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.4.3.8 | 0.0022 | - |
mutant enzyme M248I | Synechococcus sp. |
| 5.4.3.8 | 0.0123 | - |
wild type enzyme | Synechococcus sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.3.8 | (S)-4-amino-5-oxopentanoate | - |
Synechococcus sp. | 5-aminolevulinate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.3.8 | glutamate-1-semialdehyde aminomutase | - |
Synechococcus sp. |
| 5.4.3.8 | GSAM | - |
Synechococcus sp. |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.3.8 | pyridoxal 5'-phosphate | dependent on | Synechococcus sp. | |
| 5.4.3.8 | pyridoxamine 5'-phosphate | dependent on | Synechococcus sp. | |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 5.4.3.8 | 0.0028 | - |
wild type enzyme | Synechococcus sp. | gabaculine | |
| 5.4.3.8 | 0.32 | - |
mutant enzyme M248I | Synechococcus sp. | gabaculine | |
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Release 2023.1 (January 2023)
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