EC Number | Cloned (Comment) | Organism |
---|---|---|
5.4.99.16 | expressed in Escherichia coli Rosetta gami (DE3) cells | Meiothermus ruber |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.4.99.16 | Al3+ | complete inhibition at 2 mM | Meiothermus ruber | |
5.4.99.16 | Ca2+ | about 10% residual activity at 10 mM | Meiothermus ruber | |
5.4.99.16 | Co2+ | about 5% residual activity at 10 mM | Meiothermus ruber | |
5.4.99.16 | Cu2+ | complete inhibition at 2 mM | Meiothermus ruber | |
5.4.99.16 | D-glucose | in the presence of 10 mM D-glucose, TreS shows a 3.6fold increase in Km and a nearly unchanged Vmax for maltose, implying that D-glucose is a competitive inhibitor of TreS | Meiothermus ruber | |
5.4.99.16 | Fe2+ | complete inhibition at 2 mM | Meiothermus ruber | |
5.4.99.16 | Mn2+ | about 10% residual activity at 10 mM | Meiothermus ruber | |
5.4.99.16 | Ni2+ | about 10% residual activity at 10 mM | Meiothermus ruber | |
5.4.99.16 | Zn2+ | complete inhibition at 2 mM | Meiothermus ruber |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.4.99.16 | 98.9 | - |
alpha,alpha-trehalose | - |
Meiothermus ruber | |
5.4.99.16 | 126.2 | - |
maltose | - |
Meiothermus ruber |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.4.99.16 | 110000 | - |
SDS-PAGE | Meiothermus ruber |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.4.99.16 | Meiothermus ruber | B1PK99 | - |
- |
5.4.99.16 | Meiothermus ruber CBS-01 | B1PK99 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.4.99.16 | Ni-NTA column chromatography | Meiothermus ruber |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.16 | maltose | the enzyme has a 2fold higher catalytic efficiency (kcat/Km) for maltose than for trehalose indicating maltose as the preferred substrate | Meiothermus ruber | alpha,alpha-trehalose | TreS also has a weak hydrolytic property with D-glucose as the byproduct | r | |
5.4.99.16 | maltose | the enzyme has a 2fold higher catalytic efficiency (kcat/Km) for maltose than for trehalose indicating maltose as the preferred substrate | Meiothermus ruber CBS-01 | alpha,alpha-trehalose | TreS also has a weak hydrolytic property with D-glucose as the byproduct | r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.4.99.16 | Trehalose synthase | - |
Meiothermus ruber |
5.4.99.16 | TreS | - |
Meiothermus ruber |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.4.99.16 | 50 | - |
- |
Meiothermus ruber |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.4.99.16 | 60 | - |
the enzyme maintains very high activity (above 90%) at 60°C for 5 h | Meiothermus ruber |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.4.99.16 | 68.9 | - |
alpha,alpha-trehalose | - |
Meiothermus ruber | |
5.4.99.16 | 147 | - |
maltose | - |
Meiothermus ruber |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.4.99.16 | 6.5 | - |
- |
Meiothermus ruber |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
5.4.99.16 | 6 | 7 | - |
Meiothermus ruber |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
5.4.99.16 | 4 | 8 | the enzyme can maintain very high activity (above 90%) at pH 4.0-8.0 for 5 h, below pH 5.0 the enzyme shows no activity | Meiothermus ruber |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.4.99.16 | 3.76 | - |
D-glucose | - |
Meiothermus ruber |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.4.99.16 | 0.7 | - |
alpha,alpha-trehalose | - |
Meiothermus ruber | |
5.4.99.16 | 1.17 | - |
maltose | - |
Meiothermus ruber |