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Literature summary extracted from

  • Geerlof, A.; Rakels, J.J.L.; Straathof, A.J.J.; Heijnen, J.J.; Jongejan, J.A.; Duine, J.A.
    Description of the kinetic mechanism and the enantioselectivity of quinohaemoprotein ethanol dehydrogenase from Comamonas testosteroni in the oxidation of alcohols and aldehydes (1994), Eur. J. Biochem., 226, 537-546.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.9.1 4
-
 (R)-solketal pH 7.5, presence of CaCl2 Comamonas testosteroni
1.1.9.1 81
-
 (R)-solketal aldehyde pH 7.5, presence of CaCl2 Comamonas testosteroni
1.1.9.1 182
-
 (S)-solketal aldehyde pH 7.5, presence of CaCl2 Comamonas testosteroni
1.1.9.1 197
-
 Ethanal pH 7.5, presence of CaCl2 Comamonas testosteroni
1.1.9.1 211
-
 ethanol pH 7.5, presence of CaCl2 Comamonas testosteroni
1.1.9.1 302
-
 (R)-3-bromo-2-methyl-1-propanol pH 7.5, presence of CaCl2 Comamonas testosteroni

Organism

EC Number Organism UniProt Comment Textmining
1.1.9.1 Comamonas testosteroni
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.9.1 (R)-3-bromo-2-methyl-1-propanol + ferricyanide
-
 Comamonas testosteroni (R)-3-bromo-2-methyl-propanal + ferrocyanide
-
 r
1.1.9.1 (R)-solketal + 2 ferricyanide (R)-enantiomer is prefered, enantiomeric ratio is 30 Comamonas testosteroni (R)-solketal aldehyde + 2 ferrocyanide + 2 H+
-
 r
1.1.9.1 (R)-solketal aldehyde + ferrocyanide
-
 Comamonas testosteroni (R)-solketal + ferricyanide
-
 r
1.1.9.1 (R)-solketal aldehyde + ferrocyanide (R)-enantiomer is prefered, enantiomeric ratio is 2.8 for initial rate measurement Comamonas testosteroni (R)-solketal + ferricyanide
-
 r
1.1.9.1 (S)-solketal + 2 ferricyanide the second step in (S)-solketal conversion is much faster than the first one and that opposite enantiomeric preferences exist for the alcohol and the aldehyde substrates. (R)-enantiomer is prefered, enantiomeric ratio is 30 for initial rate measurement, 117 for progress curve analysis Comamonas testosteroni (S)-solketal aldehyde + 2 ferrocyanide + 2 H+
-
 r
1.1.9.1 ethanal + ferrocyanide
-
 Comamonas testosteroni ethanol + ferricyanide
-
 r
1.1.9.1 ethanol + ferricyanide
-
 Comamonas testosteroni ethanal + ferrocyanide
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.9.1 0.18
-
 (S)-solketal pH 7.5, presence of CaCl2 Comamonas testosteroni
1.1.9.1 2.12
-
 (R)-solketal pH 7.5, presence of CaCl2 Comamonas testosteroni
1.1.9.1 8.3
-
 (R)-solketal aldehyde pH 7.5, presence of CaCl2 Comamonas testosteroni
1.1.9.1 17.1
-
 (S)-solketal aldehyde pH 7.5, presence of CaCl2 Comamonas testosteroni
1.1.9.1 17.9
-
 ethanol pH 7.5, presence of CaCl2 Comamonas testosteroni
1.1.9.1 19.9
-
 Ethanal pH 7.5, presence of CaCl2 Comamonas testosteroni
1.1.9.1 20.3
-
 (R)-3-bromo-2-methyl-1-propanol pH 7.5, presence of CaCl2 Comamonas testosteroni

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.9.1 67
-
 (R)-3-bromo-2-methyl-1-propanol pH 7.5, presence of CaCl2 Comamonas testosteroni
1.1.9.1 85
-
 ethanol pH 7.5, presence of CaCl2 Comamonas testosteroni
1.1.9.1 94
-
 (S)-solketal aldehyde pH 7.5, presence of CaCl2 Comamonas testosteroni
1.1.9.1 101
-
 Ethanal pH 7.5, presence of CaCl2 Comamonas testosteroni
1.1.9.1 102
-
 (R)-solketal aldehyde pH 7.5, presence of CaCl2 Comamonas testosteroni
1.1.9.1 517
-
 (R)-solketal pH 7.5, presence of CaCl2 Comamonas testosteroni